TITLE

Photobleaching reduced fluorescence correlation spectroscopy

AUTHOR(S)
Mondal, Partha P.; Gilbert, Richard J.; So, Peter T. C.
PUB. DATE
September 2010
SOURCE
Applied Physics Letters;9/6/2010, Vol. 97 Issue 10, p103704
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A dual beam excitation-depletion pulse technique is proposed for photobleaching reduced fluorescence correlation spectroscopy (FCS). Excitation pulse promote the molecules to the excited singlet state (S1), a fraction of that population goes to energetically favorable metastable triplet state (T1) due to strong intersystem crossing. The depletion pulse followed by excitation pulse instantaneously depletes the triplet states thereby recycling the bleached molecules back to the ground state (S0). FCS study on diffusing Fluorescein and Rh6G molecules show more than 95% reduction in triplet state population and the associated photobleaching.
ACCESSION #
53507800

 

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