TITLE

A confirmatory method for the determination of phenolic endocrine disruptors in honey using restricted-access material—liquid chromatography—tandem mass spectrometry

AUTHOR(S)
Rodríguez-Gonzalo, E.; García-Gómez, D.; Carabias-Martínez, R.
PUB. DATE
October 2010
SOURCE
Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 3, p1239
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The present work describes the development and validation of an analytical method based on liquid chromatography (LC), coupled with tandem mass spectrometry (MS/MS) that allows the determination and confirmation of several endocrine-disrupting chemicals (EDCs) in honey. The EDCs studied were nine phenols of different nature: chlorophenols (2,4-dichlorophenol, 2,4,5-trichlorophenol, and pentachlorophenol), alkylphenols (4- tert-butylphenol, 4- tert-octylphenol, and 4- n-octylphenol) bisphenols (bisphenol-A and bisphenol-F), and 4- tert-butylbenzoic acid. The method incorporates a restricted-access material (RAM), coupled on-line to the LC-MS/MS system, which allows direct injection of the matrix into the RAM-LC-MS/MS system. The optimized method developed, RAM-LC-MS/MS, was applied to fortified honey samples, affording detection limits in the 0.6–7.2 ng g range, calculated for a signal-to-noise ratio of 3. In addition, the method was validated as a quantitative confirmatory method according to European Union Decision 2002/657/EC. The validation criteria evaluated were linearity, repeatability, reproducibility, recovery, decision limits, detection capabilities, specificity, and ruggedness. Repeatability and within-laboratory reproducibility were evaluated at two concentration levels, being ±11% or below at 20 ng g. The decision limits (CC) and detection capabilities (CC) were in the 1.7–12.6 and 2.8–21.6 ng g range, respectively.
ACCESSION #
53476310

 

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