TITLE

Simultaneous determination of six alkaloids in blood and urine using a hydrophilic interaction liquid chromatography method coupled with electrospray ionization tandem mass spectrometry

AUTHOR(S)
Ximei Wu; Weixiong Huang; Lin Lu; Li Lin; Xingfen Yang
PUB. DATE
October 2010
SOURCE
Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 3, p1319
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
method was developed for the simultaneous determination of six toxic alkaloids (aconitine, hypaconitine, gelsemine, raceanisodamine, strychnine, brucine) in blood and urine by hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray tandem mass spectrometry. Ephedrine was selected as the internal standard. Samples were extracted and cleaned up by solid-phase extraction (SPE) using Oasis MCX cartridges. Separation parameters such as organic modifier, buffer pH, and concentration of buffer salt were investigated. Gradient separation and analysis were achieved for six alkaloids on a 3-μm Atlantis HILIC column using a mobile phase consisting of 30 mM ammonium formate and acetonitrile at pH 3. Two multiple reaction monitoring (MRM) transitions for each substance were monitored to provide sufficient identification of alkaloid. The retention mechanisms were explored in the method development. Validation included assessment of linearity, limit of quantification, accuracy, and precision. Bias was less than 15.1% and precision was better than 8.3% for both blood and urine samples. A total of 54 clinical samples were examined by this validated method.
ACCESSION #
53476272

 

Related Articles

  • Determination of Ephedra Alkaloid and Caffeine Concentrations in Dietary Supplements and Biological Fluids. Jacob III, Peyton; Haller, Christine A.; Minjiang Duan, Christine A.; Yu, Lisa; Peng, Margaret; Benowitz, Neal L. // Journal of Analytical Toxicology;Apr2004, Vol. 28 Issue 3, p152 

    Reports on the development of a liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry (LC-MS-MS) method for quantitating the various ephedrine-group alkaloids found in dietary supplements with Ephedra, and in plasma and urine of persons consuming these...

  • Simultaneous Determination of Matrine and Berberine in Fruits, Vegetables, and Soil Using Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry. XINGANG LIU; YINGYING TIAN; FENGSHOU DONG; JUN XU; YUANBO LI; XUYANG LIANG; YUNHAO WANG; YONGQUAN ZHENG // Journal of AOAC International;Jan/Feb2014, Vol. 97 Issue 1, p218 

    An ultra-performance LC/tandem MS (UPLC/MS/MS) method for the simultaneous quantification and identification of matrine and berberine, alkaloids widely used in plant fungicides, has been developed and validated. Methanol or 1% ammonia-acetonitrile were selected as extraction solvents, and...

  • Ion Suppression in LC-MS-MS- A Case Study.  // LC-GC North America;Jan2002, Vol. 20 Issue 1, p24 

    Presents a study that dealt with ion suppression in liquid chromatography coupled with mass spectrometry. Identification of ion suppression; Information on a method for analyzing a proprietary pharmaceutical compound and one of its metabolites.

  • ( S )-2-Acetoxy-5-Undecanone, Female Sex Pheromone of the Raspberry Cane Midge, Resseliella theobaldi (Barnes). David Hall; Dudley Farman; Jerry Cross; Tetsu Ando; Masanobu Yamamoto // Journal of Chemical Ecology;Feb2009, Vol. 35 Issue 2, p230 

    Abstract  The raspberry cane midge, Resseliella theobaldi, is a widespread pest of cultivated red raspberry in Europe. Pheromone-baited traps could provide a much-needed, accurate means to monitor the pest. Volatiles collected separately from virgin female and male midges were analyzed by...

  • When Ideal Isn't Practical. Harrington, Melissa M.; Dolan, John W. // LC-GC North America;Sep2001, Vol. 19 Issue 9, p956 

    Deals with the advent of liquid chromatography (LC) systems coupled with mass spectrometry (LC-MS systems). Analytical methods for LC and LC-MS applications; Implications of the liquid extraction technique; Technique to test sample mass overload.

  • Determination of Ephedrine and Pseudoephedrine by Field-Amplified Sample Injection Capillary Electrophoresis. Deng, Dongli; Deng, Hao; Zhang, Lichun; Su, Yingying // Journal of Chromatographic Science;Apr2014, Vol. 52 Issue 4, p357 

    A simple and rapid capillary electrophoresis method was developed for the separation and determination of ephedrine (E) and pseudoephedrine (PE) in a buffer solution containing 80 mM of NaH2PO4 (pH 3.0), 15 mM of β-cyclodextrin and 0.3% of hydroxypropyl methylcellulose. The field-amplified...

  • The Changing Face of LC-MS: From Experts to Users. Plumb, Robert S.; Balogh, Michael P. // Spectroscopy;Nov2008 Current Trends in Mass Spec, p14 

    The article discusses the importance of liquid chromatography (LC) and mass spectrometry (MS) in the pharmaceutical industry. The author claims that pharmaceutical industry workers involved in drug discovery and development rely on the specificity, dynamic range, and sensitivity of MS....

  • Liquid Chromatography Coupled with Tandem Mass Spectrometry for Clinical Applications. Zonderman, Jeffrey; Shushan, Bori // Spectroscopy;Nov2008 Current Trends in Mass Spec, p25 

    The article discusses the importance of liquid chromatography coupled with tandem mass spectrometry in clinical applications. It says that liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS) is emerging as a complementary method to traditional methodology used for clinical...

  • Market Profile: LC--MS Water Analysis.  // Spectroscopy;May2011, Vol. 26 Issue 5, p10 

    The article focuses on the help of using liquid chromatography-mass spectrometry (LC-MS) on water analysis applications. It mentions that LC-MS is beneficial in monitoring and identifying contaminants found in water. It notes that the market for the use of LC-MS in water analysis has grown to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics