TITLE

Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase

AUTHOR(S)
Maeshima, Kazuhiro; Iino, Haruki; Hihara, Saera; Funakoshi, Tomoko; Watanabe, Ai; Nishimura, Masaomi; Nakatomi, Reiko; Yahata, Kazuhide; Imamoto, Fumio; Hashikawa, Tsutomu; Yokota, Hideo; Imamoto, Naoko
PUB. DATE
September 2010
SOURCE
Nature Structural & Molecular Biology;Sep2010, Vol. 17 Issue 9, p1065
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation, that cyclin-dependent kinases (Cdks), especially Cdk1 and Cdk2, promote interphase NPC formation in human dividing cells. Cdks seem to drive an early step of NPC formation because Cdk inhibition suppressed generation of 'nascent pores', which we argue are immature NPCs under the formation process. Consistent with this, Cdk inhibition disturbed proper expression and localization of some nucleoporins, including Elys/Mel-28, which triggers postmitotic NPC assembly. Strikingly, Cdk suppression did not notably affect nuclear growth, suggesting that interphase NPC formation and nuclear growth have distinct regulation mechanisms.
ACCESSION #
53473315

 

Related Articles

  • Modulating Cell Cycle: Current Applications and Prospects for Future Drug Development. Hala Gali-Muhtasib; Nadine Bakkar // Current Cancer Drug Targets;Dec2002, Vol. 2 Issue 4, p309 

    The cell cycle is a highly conserved and ordered set of events, culminating in cell growth and division. It is tightly controlled by many regulatory mechanisms that either permit or restrain its progression. The main families of regulatory proteins that play key roles in controlling cell cycle...

  • Kip1 meets SKP2: new links in cell-cycle control. Amati, Bruno; Vlach, Jaromir // Nature Cell Biology;Aug99, Vol. 1 Issue 4, pE91 

    The cyclin-dependent kinase inhibitor p27Kip1 is degraded in a phosphorylation- and ubiquitin-dependent way upon cell-cycle entry. p45SKP2, the adaptor subunit of the ubiquitin?protein ligase SCFSKP2, may mediate recognition and ubiquitination of phosphorylated p27Kip1.

  • Interferon-induces expression of cyclin-dependent kinase-inhibitors p21WAF1 and p27Kip1 that prevent activation of cyclin-dependent kinase by CDK-activating kinase (CAK). Mandal, Mahitosh; Bandyopadhyay, Debdutta; Goepfert, Thea M; Kumar, Rakesh // Oncogene;1/15/98, Vol. 16 Issue 2, p217 

    To understand the mechanism of interferon (IFN)-mediated suppression of cell cycle progression, we have earlier shown that IFN-α enhances the expression of underphosphorylated retinoblastoma protein by inhibiting the cyclin-dependent kinase-2 (CDK-2) activity (Kumar and Atlas, Proc. Natl....

  • PKB/Akt mediates cell-cycle progression by phosphorylation of p27Kip1 at threonine 157 and modulation of its cellular localization. Shin, Incheol; Yakes, F Michael; Rojo, Federico; Shin, Nah-Young; Bakin, Andrei V.; Baselga, Jose; Arteaga, Carlos L. // Nature Medicine;Oct2002, Vol. 8 Issue 10, p1145 

    We have shown a novel mechanism of Akt-mediated regulation of the CDK inhibitor p27[sup kip1]. Blockade of HER2/neu in tumor cells inhibits Akt kinase activity and upregulates nuclear levels of the CDK inhibitor p27[sup Kip1]. Recombinant Akt and Akt precipitated from tumor cells phosphorylated...

  • Differential modulation of paclitaxel-mediated apoptosis by p21Waf1 and p27Kip1. Schmidt, Mathias; Lu, Yang; Liu, Bolin; Fang, Min; Mendelsohn, John; Fan, Zhen // Oncogene;5/11/2000, Vol. 19 Issue 20, p2423 

    The impact of the cyclin dependent kinase (CDK) inhibitors p21Waf1 and p27Kip1 on paclitaxel-mediated cytotoxicity was investigated in RKO human colon adenocarcinoma cells with the ecdysone-inducible expression of p21Waf1 or p27Kip1. Ectopic expression of p27Kip1 arrested cells at G1 phase,...

  • Targets of the cyclin-dependent kinase Cdk1. Ubersax, Jeffrey A.; Woodbury, Erika L.; Quang, Phuong N.; Paraz, Maria; Blethrow, Justin D.; Shah, Kavita; Shokat, Kevan M.; Morgan, David O. // Nature;10/23/2003, Vol. 425 Issue 6960, p859 

    The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have...

  • Ubiquitin/proteasome-mediated degradation of p19INK4d determines its periodic expression during the cell cycle. Thullberg, Minna; Bartek, Jiri; Lukas, Jiri // Oncogene;6/1/2000, Vol. 19 Issue 24, p2870 

    Assembly and activity of the proto-oncogenic cyclin D/CDK4(6) complexes, the major driving force of G1 phase progression, is negatively regulated by a family of INK4 CDK inhibitors p16INK4a, p15INK4b, p18INK4c, and p19INK4d. Expression of the INK4 family members is controlled at the...

  • Cdc28 and Ime2 Possess Redundant Functions in Promoting Entry Into Premeiotic DNA Replication in.... Guttmann-Raviv, Noga; Boger-Nadjar, Elisabeth; Edri, Iris; Kassir, Yona // Genetics;Dec2001, Vol. 159 Issue 4, p1547 

    Determines the initiation of mitotic cell cycle by the sequential activity of the cyclin-dependent kinase Cdc28. Requirements for premeiotic DNA replication; Determination of nuclear divisions and asci formation; Use of structural and regulatory elements in DNA synthesis.

  • Preclinical and Clinical Development of Cyclin-Dependent Kinase Modulators. Senderowicz, Adrian M.; Sausville, Edward A. // JNCI: Journal of the National Cancer Institute;03/01/2000, Vol. 92 Issue 5, p376 

    Focuses on the discovery and cloning of cyclin-dependent kinases (cdks), key regulators of cell cycle progression. How the alteration of cdk activity occurs indirectly; Two direct cdk modulators.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics