Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging

Takahashi, Akira; Hayashi, Nagao; Miyao, Akio; Hirochika, Hirohiko
January 2010
BMC Plant Biology;2010, Vol. 10, p175
Academic Journal
Background: R gene-mediated resistance is one of the most effective mechanisms of immunity against pathogens in plants. To date some components that regulate the primary steps of plant immunity have been isolated, however, the molecular dissection of defense signaling downstream of the R proteins remains to be completed. In addition, R genes are known to be highly variable, however, the molecular mechanisms responsible for this variability remain obscure. Results: To identify novel factors required for R gene-mediated resistance in rice, we used rice insertional mutant lines, induced by the endogenous retrotransposon Tos17, in a genetic screening involving the rice blast fungus Magnaporthe oryzae. We inoculated 41,119 mutant lines with the fungus using a high throughput procedure, and identified 86 mutant lines with diminished resistance. A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site (NBS) and leucine rich repeat (LRR) domain-containing (NBS-LRR) protein. A genetic complementation analysis and a pathogenesis assay demonstrated that this NBS-LRR gene encodes Pish, which confers resistance against races of M. oryzae containing avrPish. The other 14 lines have intact copies of the Pish gene, suggesting that they may contain mutations in the signaling components downstream of Pish. The genome analysis indicated that Pish and its neighboring three NBS-LRR genes are high similar to one another and are tandemly located. An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion. Intriguingly, the insertion sites are not distributed evenly among these four NBS-LRR genes, despite their similarity at the sequence and expression levels. Conclusions: In this work we isolated the R gene Pish, and identified several other mutants involved in the signal transduction required for Pish-mediated resistance. These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice. Furthermore, our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes. Based on this finding, a possible mechanism underlying the high variability of R genes is discussed.


Related Articles

  • Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Berruyer, R.; Adreit, H.; Milazzo, J.; Gaillard, S.; Berger, A.; Dioh, W.; Lebrun, M.-H.; Tharreau, D. // Theoretical & Applied Genetics;Oct2003, Vol. 107 Issue 6, p1139 

    Rice blast disease is a major constraint for rice breeding. Nevertheless, the genetic basis of resistance remains poorly understood for most rice varieties, and new resistance genes remain to be identified. We identified the resistance gene corresponding to the cloned avirulence gene ACE1 using...

  • An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein. Jian-Quan Chen; Yi Dong; Yu-Jun Wang; Qiang Liu; Jin-Song Zhang; Shou-Yi Chen // Theoretical & Applied Genetics;Oct2003, Vol. 107 Issue 6, p972 

    We cloned an AP2/EREBP gene by dot blotting and named it OsDREBL. Analysis of its deduced amino-acid sequence indicated that this protein had a potential nuclear-localization signal, a possible acidic-activation domain and an AP2 DNA binding domain. Northern analysis showed that the transcripts...

  • Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice. Feng Qiao; Qing Yang; Chun-Lian Wang; Ying-Lun Fan; Xue-Feng Wu; Kai-Jun Zhao // Euphytica;Oct2007, Vol. 158 Issue 1/2, p35 

    GA 20-oxidase (GA20ox) is a regulatory enzyme for the syntheses of biologically active GAs in plants. The loss-of-function mutations in OsGA20ox2 of rice ( Oryza sativa L.) generate the well-known Green Revolution gene s d-1, which cause the semi-dwarfism phenotype. In our present investigation,...

  • An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence tagged microsatellite site and resistance gene analog markers. Flandez-Galvez, H.; Ford, R.; Pang, E. C. K.; Taylor, P. W. J. // Theoretical & Applied Genetics;May2003, Vol. 106 Issue 8, p1447 

    An intraspecific linkage map of the chickpea genome based on STMS as anchor markers, was established using an F2 population of chickpea cultivars with contrasting disease reactions to Ascochyta rabiei (Pass.) Lab. At a LOD-score of 2.0 and a maximum recombination distance of 20 cM, 51 out of 54...

  • Genome watch: Crops and robbers. Petty/, Nicola K. // Nature Reviews Microbiology;Oct2008, Vol. 6 Issue 10, p716 

    This month's Genome Watch discusses the genome sequences of pathogenic and commensal bacteria that are associated with plant crops and shows how analysis of such genomes can lead to new approaches to combat plant diseases.

  • Construction of two Lolium perenne BAC libraries and identification of BACs containing candidate genes for disease resistance and forage quality. Farrar, Kerrie; Asp, Torben; Lübberstedt, Thomas; Mingliang Xu; Thomas, Ann M.; Christiansen, Camilla; Humphreys, Mervyn O.; Donnison, Iain S. // Molecular Breeding;Jan2007, Vol. 19 Issue 1, p15 

    Two BAC libraries were constructed for the forage and turf grass species Lolium perenne L. The libraries consisted of 98,304 and 101,376 BAC clones for L. perenne genotypes LTS18 and NV#20F1-30, respectively. The estimated average insert size of both libraries was approximately 100 Kb and L....

  • NLR-parser: rapid annotation of plant NLR complements. Steuernagel, Burkhard; Jupe, Florian; Witek, Kamil; Jones, Jonathan D. G.; Wulff, Brande B. H. // Bioinformatics;5/15/2015, Vol. 31 Issue 10, p1665 

    Motivation: The repetitive nature of plant disease resistance genes encoding for nucleotidebinding leucine-rich repeat (NLR) proteins hampers their prediction with standard gene annotation software. Motif alignment and search tool (MAST) has previously been reported as a tool to support...

  • A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance. Moroldo, Marco; Paillard, Sophie; Marconi, Raffaella; Fabrice, Legeai; Canaguier, Aurelie; Cruaud, Corinne; De Berardinis, Veronique; Guichard, Cecile; Brunaud, Veronique; Le Clainche, Isabelle; Scalabrin, Simone; Testolin, Raffaele; Di Gaspero, Gabriele; Morgante, Michele; Adam-Blondon, Anne-Francoise // BMC Plant Biology;2008, Vol. 8, Special section p1 

    Background: Whole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed...

  • Functional analysis of Xa3/Xa26 family members in rice resistance to Xanthomonas oryzae pv. oryzae. Yinglong Cao; Liu Duan; Hongjing Li; Xinli Sun; Yu Zhao; Caiguo Xu; Xianghua Li; Shiping Wang // Theoretical & Applied Genetics;Nov2007, Vol. 115 Issue 7, p887 

    Plant disease resistant ( R) genes are frequently clustered in the genome. The diversity of members in a complex R-gene family may provide variation in resistance specificity. Rice Xa3/Xa26, conferring resistance to Xanthomonas oryzae pv. oryzae ( Xoo) encodes a leucine-rich repeat (LRR)...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics