TITLE

Rapid modification of the insect elicitor N-linolenoyl-glutamate via a lipoxygenase-mediated mechanism on Nicotiana attenuata leaves

AUTHOR(S)
VanDoorn, Arjen; Kallenbach, Mario; Borquez, Alejandro A.; Baldwin, Ian T.; Bonaventure, Gustavo
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p164
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Some plants distinguish mechanical wounding from herbivore attack by recognizing specific constituents of larval oral secretions (OS) which are introduced into plant wounds during feeding. Fatty acid-amino acid conjugates (FACs) are major constituents of Manduca sexta OS and strong elicitors of herbivore-induced defense responses in Nicotiana attenuata plants. Results: The metabolism of one of the major FACs in M. sexta OS, N-linolenoyl-glutamic acid (18:3-Glu), was analyzed on N. attenuata wounded leaf surfaces. Between 50 to 70% of the 18:3-Glu in the OS or of synthetic 18:3- Glu were metabolized within 30 seconds of application to leaf wounds. This heat-labile process did not result in free α-linolenic acid (18:3) and glutamate but in the biogenesis of metabolites both more and less polar than 18:3- Glu. Identification of the major modified forms of this FAC showed that they corresponded to 13-hydroxy-18:3-Glu, 13-hydroperoxy-18:3-Glu and 13-oxo-13:2-Glu. The formation of these metabolites occurred on the wounded leaf surface and it was dependent on lipoxygenase (LOX) activity; plants silenced in the expression of NaLOX2 and NaLOX3 genes showed more than 50% reduced rates of 18:3-Glu conversion and accumulated smaller amounts of the oxygenated derivatives compared to wild-type plants. Similar to 18:3-Glu, 13-oxo-13:2-Glu activated the enhanced accumulation of jasmonic acid (JA) in N. attenuata leaves whereas 13-hydroxy-18:3-Glu did not. Moreover, compared to 18:3-Glu elicitation, 13-oxo-13:2-Glu induced the differential emission of two monoterpene volatiles (b-pinene and an unidentified monoterpene) in irlox2 plants. Conclusions: The metabolism of one of the major elicitors of herbivore-specific responses in N. attenuata plants, 18:3-Glu, results in the formation of oxidized forms of this FAC by a LOX-dependent mechanism. One of these derivatives, 13-oxo-13:2-Glu, is an active elicitor of JA biosynthesis and differential monoterpene emission.
ACCESSION #
53459089

 

Related Articles

  • In vitro Protoplast Isolation and Regeneration of Solanum tuberosum cv. Binella and Burren. Al-Maarri, K.; Al-Qabbani, N.; Al-Biski, F. // Jordan Journal of Agricultural Sciences;2014, Vol. 10 Issue 1, p32 

    The present study aims to develop simple reliable method for protoplast isolation, culture and regeneration from the leaves of potato cvs. Binella and Burren. Optimal conditions for protoplast culture, callus initiation and regeneration were determined. Moreover, genetic diversity between...

  • Investigations into plant biochemical wound-response pathways involved in the production of aphid-induced plant volatiles. Girling, Robbie D.; Madison, Rachael; Hassall, Mark; Poppy, Guy M.; Turner, John G. // Journal of Experimental Botany;Aug2008, Vol. 59 Issue 11, p3077 

    Feeding damage to plants by insect herbivores induces the production of plant volatiles, which are attractive to the herbivores natural enemies. Little is understood about the plant biochemical pathways involved in aphid-induced plant volatile production. The aphid parasitoid Diaeretiella rapae...

  • Unbiased Transcriptional Comparisons of Generalist and Specialist Herbivores Feeding on Progressively Defenseless Nicotiana attenuata Plants. Govind, Geetha; Mittapalli, Omprakash; Griebel, Thasso; Allmann, Silke; Böcker, Sebastian; Baldwin, Ian Thomas // PLoS ONE;2010, Vol. 5 Issue 1, p1 

    Background: Herbivore feeding elicits dramatic increases in defenses, most of which require jasmonate (JA) signaling, and against which specialist herbivores are thought to be better adapted than generalist herbivores. Unbiased transcriptional analyses of how neonate larvae cope with these...

  • Silencing an N-Acyltransferase-Like Involved in Lignin Biosynthesis in Nicotiana attenuata Dramatically Alters Herbivory-Induced Phenolamide Metabolism Gaquerel, Emmanuel; Kotkar, Hemlata; Onkokesung, Nawaporn; Galis, Ivan; Baldwin, Ian T. // PLoS ONE;May2013, Vol. 8 Issue 5, p1 

    In a transcriptomic screen of Manduca sexta-induced N-acyltransferases in leaves of Nicotiana attenuata, we identified an N-acyltransferase gene sharing a high similarity with the tobacco lignin-biosynthetic hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) gene whose...

  • Isolating Fungal Pathogens from a Dynamic Disease Outbreak in a Native Plant Population to Establish Plant-Pathogen Bioassays for the Ecological Model Plant Nicotiana attenuata. Schuck, Stefan; Weinhold, Arne; Luu, Van Thi; Baldwin, Ian T. // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    The wild tobacco species Nicotiana attenuata has been intensively used as a model plant to study its interaction with insect herbivores and pollinators in nature, however very little is known about its native pathogen community. We describe a fungal disease outbreak in a native N. attenuata...

  • RHS help & advice. Hunt, Leigh // Garden;Nov2006, Vol. 131 Issue 11, p782 

    The article offers advice related to gardening and horticulture. It outlines the ways to alleviate the stress posed by shorter days combined with central heating on house and citrus plants. Tips on regenerating fruit trees are provided. The symptoms and the ways to control fungal turf diseases...

  • Diplodia Shoot Blight and Stem Canker.  // Arbor Age;Dec2005, Vol. 25 Issue 12, p10 

    This article presents information on the fungus Diplodia pinea, which is considered to be a serious threat to pines that are planted in landscapes and plantation settings. In many cases, this fungus deforms the tree and ultimately resulting in its death. The most common symptoms of the fungus...

  • Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts. Farmaki, Theodora; Sanmartín, Maite; Jiménez, Pedro; Paneque, Manuel; Sanz, Carlos; Vancanneyt, Guy; León, José; Sánchez-Serrano, Jose J. // Journal of Experimental Botany;Feb2007, Vol. 58 Issue 3, p555 

    The lipoxygenase pathway is responsible for the production of oxylipins, which are important compounds for plant defence responses. Jasmonic acid, the final product of the allene oxide synthase/allene oxide cyclase branch of the pathway, regulates wound-induced gene expression. In contrast, C6...

  • Genetic structure of European accessions of Solanum dulcamara L. (Solanaceae). Golas, T. M.; Feron, R. M. C.; van den Berg, R. G.; van der Weerden, G. M.; Mariani, C.; Allefs, J. J. H. M. // Plant Systematics & Evolution;Mar2010, Vol. 285 Issue 1/2, p103 

    Solanum dulcamara (bittersweet) is one of the few native species of Solanum present in Europe. It is a common weed that occupies a wide range of habitats and is often found in the direct vicinity of cultivated potatoes ( Solanum tuberosum), where it could transmit diseases. A broad sampling of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics