TITLE

A SNP associated with alternative splicing of RPT5b causes unequal redundancy between RPT5a and RPT5b among Arabidopsis thaliana natural variation

AUTHOR(S)
Guyon-Debast, Anouchka; Lécureuil, Alain; Bonhomme, Sandrine; Guerche, Philippe; Gallois, Jean-Luc
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p158
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: The proteasome subunit RPT5, which is essential for gametophyte development, is encoded by two genes in Arabidopsis thaliana; RPT5a and RPT5b. We showed previously that RPT5a and RPT5b are fully redundant in the Columbia (Col-0) accession, whereas in the Wassilewskia accession (Ws-4), RPT5b does not complement the effect of a strong rpt5a mutation in the male gametophyte, and only partially complements rpt5a mutation in the sporophyte. RPT5bCol-0 and RPT5bWs-4 differ by only two SNPs, one located in the promoter and the other in the seventh intron of the gene. Results: By exploiting natural variation at RPT5b we determined that the SNP located in RPT5b intron seven, rather than the promoter SNP, is the sole basis of this lack of redundancy. In Ws-4 this SNP is predicted to create a new splicing branchpoint sequence that induces a partial mis-splicing of the pre-mRNA, leading to the introduction of a Premature Termination Codon. We characterized 5 accessions carrying this A-to-T substitution in intron seven and observed a complete correlation between this SNP and both a 10 to 20% level of the RPT5b pre-mRNA missplicing and the lack of ability to complement an rpt5a mutant phenotype. Conclusion: The accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing.
ACCESSION #
53456766

 

Related Articles

  • Physiological basis of QTLs for boron efficiency in Arabidopsis thaliana. Changying Zeng; Fangsen Xu; Yunhua Wang; Chengxiao Hu; Jinling Meng // Plant & Soil;Jul2007, Vol. 296 Issue 1/2, p187 

    Boron (B) is an essential micronutrient for higher plants, but the adaptability of plants to B deficiency varies widely both between and within species. On the basis of quantitative trait loci (QTL) analysis of the B efficiency coefficient (BEC) detected in an Arabidopsis thaliana L er × Col...

  • Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit. Wawrzyñska, Anna; Lewandowska, Małgorzata; Hawkesford, Malcolm J.; Sirko, Agnieszka // Journal of Experimental Botany;Jun2005, Vol. 56 Issue 416, p1575 

    Monitoring expression at the transcriptional level is an essential first step for the functional analysis of plant genes. Genes encoding proteins directly involved in sulphur metabolism constitute only a small fraction of all the genes affected by sulphur deficiency stress. Transcriptional...

  • Extending the model of Arabidopsis telomere length and composition across Brassicaceae. Nelson, Andrew; Forsythe, Evan; Gan, Xiangchao; Tsiantis, Miltos; Beilstein, Mark // Chromosome Research;Jun2014, Vol. 22 Issue 2, p153 

    Telomeres are repetitive TG-rich DNA elements essential for maintaining the stability of genomes and replicative capacity of cells in almost all eukaryotes. Most of what is known about telomeres in plants comes from the angiosperm Arabidopsis thaliana, which has become an important comparative...

  • Interaction between the PINOID/ABRUPTUS gene with the AGAMOUS gene negatively regulating stem cells proliferetion in the Arabidopsis thaliana floral meristem. Kavai-ool, U. N.; Karpenko, O. Yu.; Ezhova, T. A. // Russian Journal of Developmental Biology;Mar2011, Vol. 42 Issue 2, p120 

    Complementary interaction between the AGAMOUS and PINOID/ABRUPTUS genes was revealed. In double mutant abr ag-1, there is a significant increasing proliferation of cells of the floral meristem and formation of branching shoot-like flowers. These data indicate the participation of the PID/ABR...

  • Genomic Survey of Gene Expression Diversity in Arabidopsis thaliana. Kliebenstein, Daniel J.; West, Marilyn A. L.; Van Leeuwen, Hans; Kyunga Kim; Doerge, R. W.; Michelmore, Richard W.; Clair, Dina A. St. // Genetics;Feb2006, Vol. 172 Issue 2, p1179 

    Differential gene expression controls variation in numerous plant traits, such as flowering time and plant/pest interactions, but little is known about the genomic distribution of the determinants of transcript levels and their associated variation. Affymetrix ATH1 GeneChip microarrays...

  • Light responses of a plastic plant. Millar, Andrew J. // Nature Genetics;Dec2001, Vol. 29 Issue 4, p357 

    Arabidopsis thaliana isolates from the wild vary enormously in their morphology and physiological responses to standard conditions, indicating that substantial genetic variation is accessible in this species. Natural variant alleles encoding two Arabidopsis photoreceptors, phytochrome A and...

  • Plant genetics: a decade of integration. Pruitt, Robert E.; Bowman, John L.; Grossniklaus, Ueli // Nature Genetics;Mar2003 Supplement, Vol. 33, p294 

    The last decade provided the plant science community with the complete genome sequence of Arabidopsis thaliana and rice, tools to investigate the function of potentially every plant gene, methods to dissect virtually any aspect of the plant life cycle, and a wealth of information on gene...

  • Genetic Architecture of Plastic Methyl Jasmonate Responses in Arabidopsis thaliana. Kliebenstein, Daniel J.; Figuth, Antje; Mitchell-Olds, Thomas // Genetics;Aug2002, Vol. 161 Issue 4, p1685 

    Examines the genetic architecture of plastic methyl jasmonate (MeJa) responses in Arabidopsis thaliana. Analysis of MeJa and salicylic acid cross-talk in glucosinolate regulation; Implications for glucosinolate physiology and functional understanding of Arabidopsis MeJa signal transduction.

  • Genetic variation within and among populations of Arabidopsis thaliana. Bergelson, Joy; Stahl, Eli; Dudek, Scott; Kreitman, Martin // Genetics;Mar98, Vol. 148 Issue 3, p1311 

    Presents a study which examined the genetic variation of Arabidopsis thaliana. Examination of the levels of nucleotide polymorphism within Arabidopsis thaliana; Method employed in this study; Results of this study.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics