TITLE

Identification and structural characterization of FYVE domain-containing proteins of Arabidopsis thaliana

AUTHOR(S)
Wywial, Ewa; Singh, Shaneen M.
PUB. DATE
January 2010
SOURCE
BMC Plant Biology;2010, Vol. 10, p157
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: FYVE domains have emerged as membrane-targeting domains highly specific for phosphatidylinositol 3-phosphate (PtdIns(3)P). They are predominantly found in proteins involved in various trafficking pathways. Although FYVE domains may function as individual modules, dimers or in partnership with other proteins, structurally, all FYVE domains share a fold comprising two small characteristic double-stranded bsheets, and a C-terminal α-helix, which houses eight conserved Zn2+ ion-binding cysteines. To date, the structural, biochemical, and biophysical mechanisms for subcellular targeting of FYVE domains for proteins from various model organisms have been worked out but plant FYVE domains remain noticeably under-investigated. Results: We carried out an extensive examination of all Arabidopsis FYVE domains, including their identification, classification, molecular modeling and biophysical characterization using computational approaches. Our classification of fifteen Arabidopsis FYVE proteins at the outset reveals unique domain architectures for FYVE containing proteins, which are not paralleled in other organisms. Detailed sequence analysis and biophysical characterization of the structural models are used to predict membrane interaction mechanisms previously described for other FYVE domains and their subtle variations as well as novel mechanisms that seem to be specific to plants. Conclusions: Our study contributes to the understanding of the molecular basis of FYVE-based membrane targeting in plants on a genomic scale. The results show that FYVE domain containing proteins in plants have evolved to incorporate significant differences from those in other organisms implying that they play a unique role in plant signaling pathways and/or play similar/parallel roles in signaling to other organisms but use different protein players/signaling mechanisms.
ACCESSION #
53456763

 

Related Articles

  • Agrobacterium tumefaciens-mediated transformation of Cleome gynandra L., a C4 dicotyledon that is closely related to Arabidopsis thaliana. Newell, Christine A.; Brown, Naomi J.; Liu, Zheng; Pflug, Alexander; Gowik, Udo; Westhoff, Peter; Hibberd, Julian M. // Journal of Experimental Botany;Apr2010, Vol. 61 Issue 5, p1311 

    In leaves of most C4 plants, the biochemistry of photosynthesis is partitioned between mesophyll and bundle sheath cells. In addition, their cell biology and development also differs from that in C3 plants. We have a poor understanding of the mechanisms that generate the cell-specific...

  • Genes encoding ADP-ribosylation factors in Arabidopsis thaliana L. Heyn.; genome analysis and antisense suppression. Gebbie, Leigh K.; Burn, Joanne E.; Hocart, Charles H.; Williamson, Richard E. // Journal of Experimental Botany;Apr2005, Vol. 56 Issue 414, p1079 

    Vesicle trafficking delivers proteins to intracellular and extracellular compartments, cellulose synthase to the plasma membrane, and non-cellulosic polysaccharides to the cell wall. The Arabidopsis genome potentially encodes 19 proteins with sequence similarities to ARFs (ADP-ribosylation...

  • Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine. tålberg, Kjell; Ståhl, Ulf; Stymne, Sten; Ohlrogge, John // BMC Plant Biology;2009, Vol. 9, Special section p1 

    Background: Two previously uncharacterized Arabidopsis genes that encode proteins with acyltransferase PlsC regions were selected for study based on their sequence similarity to a recently identified lung lysophosphatidylcholine acyltransferase (LPCAT). To identify their substrate specificity...

  • Alternative splicing at NAGNAG acceptors in Arabidopsis thaliana SR and SR-related protein-coding genes. Schindler, Stefanie; Szafranski, Karol; Hiller, Michael; Gul Shad Ali; Palusa, Saiprasad G.; Backofen, Rolf; Platzer, Matthias; Reddy, Anireddy S. N. // BMC Genomics;2008, Vol. 9, Special section p1 

    Background: Several recent studies indicate that alternative splicing in Arabidopsis and other plants is a common mechanism for post-transcriptional modulation of gene expression. However, few analyses have been done so far to elucidate the functional relevance of alternative splicing in higher...

  • Epigenetic regulation of peanut allergen gene Ara h 3 in developing embryos. Guohua Fu; Yujuan Zhong; Chenlong Li; Yin Li; Xiaodong Lin; Bin Liao; Edward Tsang; Keqiang Wu; Shangzhi Huang // Planta;Apr2010, Vol. 231 Issue 5, p1049 

    Peanut ( Arachis hypogaea) allergy is one of the most serious food allergies. Peanut seed protein, Ara h 3, is considered to be one of the most important peanut allergens. Little is known about the temporal and spatial regulation mechanism of Ara h 3 during seed development. In this study,...

  • RESISTANCE TO FUSARIUM OXYSPORUM 1, a Dominant Arabidopsis Disease-Resistance Gene, Is Not Race Specific. Diener, Andrew C.; Ausubel, Frederick M. // Genetics;Sep2005, Vol. 171 Issue 1, p305 

    Arabidopsis thaliana ecotypes differ in their susceptibility to Fusarium wilt diseases. Ecotype Taynuilt-0 (Ty-0) is susceptible to Fusarium oxysporum forma specialis (f.) matthioli whereas Columbia-0 (Col-0) is resistant. Segregation analysis of a cross between Ty-0 and Col-0 revealed six...

  • The ELF4 gene controls circadian rhythms and flowering time in Arabidopsis thaliana. Doyle, Mark R.; Davis, Seth J.; Bastow, Ruth M.; McWatters, Harriet G.; Kozma-Bognár, László; Nagy, Ferenc; Millar, Andrew J.; Amasino, Richard M. // Nature;9/5/2002, Vol. 419 Issue 6902, p74 

    Describes the early flowering 4 (ELF4) gene and its role in the control of the circadian rhythm and flowering time of the Arabidopsis thaliana. Promotion of clock accuracy and sustained rhythms in the absence of daily light/dark cycles; Function of the circadian clock associated 1 gene expressed...

  • Light responses of a plastic plant. Millar, Andrew J. // Nature Genetics;Dec2001, Vol. 29 Issue 4, p357 

    Arabidopsis thaliana isolates from the wild vary enormously in their morphology and physiological responses to standard conditions, indicating that substantial genetic variation is accessible in this species. Natural variant alleles encoding two Arabidopsis photoreceptors, phytochrome A and...

  • Plant genetics: a decade of integration. Pruitt, Robert E.; Bowman, John L.; Grossniklaus, Ueli // Nature Genetics;Mar2003 Supplement, Vol. 33, p294 

    The last decade provided the plant science community with the complete genome sequence of Arabidopsis thaliana and rice, tools to investigate the function of potentially every plant gene, methods to dissect virtually any aspect of the plant life cycle, and a wealth of information on gene...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics