Effect of the feeding system on the fatty acid composition, expression of the Δ9-desaturase, Peroxisome Proliferator-Activated Receptor Alpha, Gamma, and Sterol Regulatory Element Binding Protein 1 genes in the semitendinous muscle of light lambs of the Rasa Aragonesa breed

Dervishi, Elda; Serrano, Carmen; Joy, Margalida; Serrano, Malena; Rodellar, Clementina; Calvo, Jorge H.
January 2010
BMC Veterinary Research;2010, Vol. 6, p40
Academic Journal
Background: Conjugated linoleic acids (CLAs) are receiving increasing attention because of their beneficial effects on human health, with milk and meat products derived from ruminants as important sources of CLA in the human diet. SCD gene is responsible for some of the variation in CLA concentration in adipose tissues, and PPARγ, PPARα and SREBP1 genes are regulator of SCD gene. The aim of this work was to evaluate the effect of the feeding system on fatty acid composition, CLA content and relative gene expression of Δ9-desaturase (SCD), Peroxisome Proliferator-Activated Receptor Gamma (PPARγ), Peroxisome Proliferator-Activated Receptor Alpha, (PPARα) and Sterol Regulatory Element Binding Protein (SREBP1) in Rasa Aragonesa light lambs in semitendinous muscle. Forty-four single-born male lambs were used to evaluate the effect of the feeding system, varying on an intensity gradient according to the use of concentrates: 1. grazing alfalfa, 2. grazing alfalfa with a supplement for lambs, 3. indoor lambs with grazing ewes and 4. drylot. Results: Both grazing systems resulted in a higher concentration of vaccenic acid (VA), CLA, CLA/VA acid ratio, and a lower oleic content, oleic acid (C18:1)/stearic acid (C18:0) ratio, PUFA n-6/n-3 ratio and SCD expression compared to other diets. In addition feeding system affected the fatty acid composition and SCD expression, possibly due to CLA concentration or the PUFA n-6/n-3 ratio. Both expression of the SCD gene and the feeding system were important factors affecting CLA concentration in the animal's semitendinous muscle. PPARγ, PPARα and SREBP1 expression seemed to be unaffected by the feeding system. Although no significant results were found, PPARγ, PPARα and SREBP1 showed similar expression pattern as SCD. Moreover, the correlation results between SCD expression and PPARγ (p < 0.01), as well as SREBP1 (p < 0.01) expression, may suggest that these genes were affecting SCD expression in a different way. Conclusions: The data indicated that the feeding system is the main factor affecting the fatty acid composition and SCD gene expression, which is also affected by CLA and possibly by n-6/n-3 PUFAs.


Related Articles

  • Meat Science and Muscle Biology.  // Poultry Science;2004 Annual Meeting Abstracts, Vol. 83, p134 

    Presents abstracts of several studies about meat science and muscle biology. "Conjugated Linoleic Acid (CLA) Concentrations in Beef Tissues from Cattle Finished on Pasture Initially with Limited Grain," by R. N. Sonon, Jr., D. C. Beitz, A. H. Trenkle, J. R. Russell, and R. Rosmann; "Effects of...

  • Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Maia, Margarida R. G.; Chaudhary, Lal C.; Bestwick, Charles S.; Richardson, Anthony J.; McKain, Nest; Larson, Tony R.; Graham, Ian A.; Wallace, Robert J. // BMC Microbiology;2010, Vol. 10, p52 

    Background: Health-promoting polyunsaturated fatty acids (PUFA) are abundant in forages grazed by ruminants and in vegetable and fish oils used as dietary supplements, but only a small proportion of PUFA finds its way into meat and milk, because of biohydrogenation in the rumen. Butyrivibrio...

  • Moderate doses of conjugated linoleic acid reduce fat gain, maintain insulin sensitivity without impairing inflammatory adipose tissue status in mice fed a high-fat diet. Parra, Pilar; Palou, Andreu; Serra, Francisca // Nutrition & Metabolism;2010, Vol. 7, p5 

    Background: The enrichment of diet with nutrients with potential benefits on body composition is a strategy to combat obesity. Conjugated linoleic acid (CLA) due its beneficial effects on body composition and inflammatory processes becomes an interesting candidate, since the promotion and...

  • Feeding modulation of the fatty acid composition in lamb meat. Klir, Ž.; Antunović, Z.; Halas, V.; Domaćinović, M.; Šperanda, M.; Novoselec, J. // MESO;Jan2012, Vol. 14 Issue 1, p70 

    Various science researches have shown that meat of ruminants has more desirable fatty acid composition and a ratio between ω-6 and ω-3 fatty acids (lower than 4.0) because of lower content of linoleic, and a higher content of ω-3 polyunsaturated fatty acids, especially linolenic...

  • The effect of conjugated linoleic acid on the fatty acid composition of different tissues and yolk lipids in pigeons. Aydin, R. // South African Journal of Animal Science;2005, Vol. 35 Issue 4, p253 

    Dietary conjugated linoleic acid (CLA) was reported to increase the levels of saturated fatty acids (SFA), to decrease monounsaturated fatty acids (MUFA) in egg yolk, liver and adipose tissue in chicken and quail. Hence, the objective of this study was to determine the effects of dietary CLA on...

  • Study Finds CIA Helps Trim Body Fat, But Are Supplements Safe?  // Environmental Nutrition;Jul2007, Vol. 30 Issue 7, p3 

    The article assesses the effects of conjugated linoleic acid (CLA) supplements on the human body. It is considered that this fatty acid may help reduce body fat. However, some studies suggest that CLA supplements may worsen insulin resistance and inflammation. It was also found that this...

  • The skinny on a supposed fat-fighter.  // Consumer Reports on Health;Sep2007, Vol. 19 Issue 9, p7 

    The article examines the health benefits of conjugated linoleic acid (CLA). It is a polyunsaturated fat that comes from beef and dairy products. Animal studies consider that CLA supplements reduce body fats and increase muscle mass. Another study also determined that users lost ten pounds of...

  • Synergistic effect of synthetic conjugated linoleic acid & non fat milk on fat deposition & lipid metabolism in mice. Sagwal, R.; Kansal, V. K. // Indian Journal of Medical Research;Mar2010, Vol. 131 Issue 3, p449 

    Background & objectives: Conjugated linoleic acid (CLA) reduces fat deposition in the body, but the mechanism of action is not clear. The present study was conducted to investigate the effects of CLA on body fat metabolism. Since milk fat is the best natural source of dietary CLA, intervention...

  • Influences of the feeding ecology on body mass and possible implications for reproduction in the edible dormouse (Glis glis). Fietz, Joanna; Pflug, M.; Schlund, W.; Tataruch, F. // Journal of Comparative Physiology B: Biochemical, Systemic & Env;Jan2005, Vol. 175 Issue 1, p45 

    The edible dormouse (Glis glis) is a small rodent and an obligate hibernator. Dormice undergo strong fluctuations of reproductive output during years that seem to be timed to coincide with future food supply. This behaviour enables them to avoid producing young that will starve with a high...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics