Lab-on-a-chip flow cytometer employing color-space-time coding

Cho, Sung Hwan; Qiao, Wen; Tsai, Frank S.; Yamashita, Kenichi; Lo, Yu-Hwa
August 2010
Applied Physics Letters;8/30/2010, Vol. 97 Issue 9, p093704
Academic Journal
We describe a fluorescent detection technique for a lab-on-a-chip flow cytometer. Fluorescent emission is encoded into a time-dependent signal as a fluorescent cell or bead traverses a waveguide array with integrated spatial filters and color filters. Different from conventional colored filters with well-defined transmission spectral window, the integrated color filters are designed to have broad transmission characteristics, similar to the red-green-blue photoreceptors in the retina of human eye. This unique design allows us to detect multiple fluorescent colors with only three color filters based on the technique of color-space-time coding using only one single photomultiplier tube or avalanche photodetector.


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