Subphases of DNA Replication in Drosophila Cells

Rehak, Marian; Csuka, Ildiko; Szepessy, Edit; Banfalvi, Gaspar
October 2000
DNA & Cell Biology;Oct2000, Vol. 19 Issue 10, p607
Academic Journal
Exponentially growing Drosophila S2 cells in suspension culture were synchronized at low- and high-resolution centrifugal elutriation, and DNA synthesis was measured by [[sup 3]H]-thymidine incorporation throughout the S phase. At low resolution, one repair peak at the G[sub 1]/G[sub 0] border and two replication peaks known as early and late S subphases were observed. At high resolution, six chronologic compartments were distinguished. The distribution of these peaks indicated one repair peak at 2.05 C value, one minor replication peak at 2.43C, and four major subphases of replication corresponding to 2.64C, 2.89C, 3.32C, and 3.60C, representing 6.7%, 3.4%, 15.3%, 20.4%, 32.1%, and 22.0% of the synthetic activity, respectively. The five major peaks of cell growth with 2.32C, 2.56C, 2.85C, 3.18C, and 3.58C values consistently preceded those of replication subphases.


Related Articles

  • Role for a Drosophila Myb-containing protein complex in site-specific DNA replication. Beall, Eileen L.; Manak, J. Robert; Zhou, Sharleen; Bell, Maren; Lipsick, Joseph S.; Botchan, Michael R. // Nature;12/19/2002-12/26/2002, Vol. 420 Issue 6917, p833 

    There is considerable interest in the developmental, temporal and tissue-specific patterns of DNA replication in metazoans. Site-specific DNA replication at the chorion loci in Drosophila follicle cells leads to extensive gene amplification, and the organization of the cis-acting DNA elements...

  • DNA replication and repair. Eggleston, Angela K. // Nature;6/21/2007, Vol. 447 Issue 7147, p923 

    The article discusses various reports published within the issue of "Nature Insight," covering aspects of deoxyribonucleic acid (DNA) repair and replication from telomeres to the packaging of cellular DNA in a proteinaceous sheath.

  • Phosphothreonine recognition comes into focus. Zhou, Ming-Ming // Nature Structural Biology;Dec2000, Vol. 7 Issue 12, p1085 

    Deals with a study which discovered a mechanism governing phosphothreonine-dependent protein-protein interactions in nuclear processes including DNA repair and replication in a broad range of organisms. Function of forkhead-associated domains; Sequence preference; Phosphothreonine recognition.

  • Molecular characterization of mutant alleles of the DNA repair/basal transcription factor... Mounkes, Leslie C.; Fuller, Margaret T. // Genetics;May99, Vol. 152 Issue 1, p291 

    Focuses on the molecular characterization of mutations that identify regions of haywire in Drosophila that are important either for viability or DNA repair. Element encoded by the haywire gene of the Drosophila; Sequence analysis of lethal haywire alleles.

  • DNA Ligases: Structure, Function and Mechanism. Weiguo Cao // Current Organic Chemistry;Aug2002, Vol. 6 Issue 9, p827 

    DNA ligases play an essential role in DNA replication, repair, and recombination and are classified as ATP-dependent or NAD-dependent based on their adenylation cofactor requirement. ATP-dependent ligases have been found in bacteriophages, viruses, archaea, eukaryotes and bacteria. NAD-dependent...

  • Disruption of dog-1 in Caenorhabditis elegans triggers deletions upstream of guanine-rich DNA. Cheung, Iris; Schertzer, Michael; Rose, Ann; Lansdorp, Peter M. // Nature Genetics;Aug2002, Vol. 31 Issue 4, p405 

    Genetic integrity is crucial to normal cell function, and mutations in genes required for DNA replication and repair underlie various forms of genetic instability and disease, including cancer¹. One structural feature of intact genomes is runs of homopolymeric dC/dG. Here we describe an...

  • Some assembly required. Kowalczykowski, Stephen C. // Nature Structural Biology;Dec2000, Vol. 7 Issue 12, p1087 

    Deals with a study which demonstrated that the eukaryotic single-stranded DNA-binding protein called replication protein-A (RPA) contributes to the orchestration of sequential events in DNA recombination and repair. Mechanism of RPA; Function of single-stranded DNA binding proteins in DNA...

  • Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells. Tauchi, Hiroshi; Kobayashi, Junya; Morishima, Ken-ichi; van Gent, Dik C.; Shiraishi, Takahiro; Verkaik, Nicole S.; vanHeems, Diana; Ito, Emi; Nakamura, Asako; Sonoda, Eiichiro; Takata, Minoru; Takeda, Shunichi; Matsuura, Shinya; Komatsu, Kenshi // Nature;11/7/2002, Vol. 420 Issue 6911, p93 

    Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. There are at least two pathways which can repair such breaks: non-homologous end joining and homologous recombination (HR). Although these pathways are essentially independent of one another, it is...

  • Accessibility of the Drosophila genome discriminates PcG repression, H4K16 acetylation and replication timing. Bell, Oliver; Schwaiger, Michaela; Oakeley, Edward J; Lienert, Florian; Beisel, Christian; Stadler, Michael B.; Schübeler, Dirk // Nature Structural & Molecular Biology;Jul2010, Vol. 17 Issue 7, p894 

    Histone modifications are thought to regulate gene expression in part by modulating DNA accessibility. Here, we measured genome-wide DNA accessibility in Drosophila melanogaster by combining M.SssI methylation footprinting with methylated DNA immunoprecipitation. We show that methylase...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics