TITLE

DNA Sequence Requirements for the Activation of 434 P[sub RM] Transcription by 434 Repressor

AUTHOR(S)
Xu, Jian; Koudelka, Gerald B.
PUB. DATE
October 2000
SOURCE
DNA & Cell Biology;Oct2000, Vol. 19 Issue 10, p621
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A dimer of the 434 repressor bound at O[sub R]2 activated transcription initiation from P[sub RM] by contacting RNA polymerase. Although DNA-binding site mutations at either end of O[sub R]2 decreased the ability of the repressor to activate P[sub RM] transcription, mutations proximal to the promoter had a greater effect on transcription activation. Orienting a repressor subunit bearing the altered specificity Gln-28 → Ala mutation to the halfsite of O[sub R]2 proximal to the P[sub RM] promoter decreased the repressor's ability to activate transcription initiation at 434 P[sub RM] to a much greater extent than if this subunit was placed in the O[sub R]2 half-site distal to P[sub RM]. In addition to showing that the downstream (promoter proximal) subunit of the O[sub R]2-bound 434 repressor functions in activating 434 P[sub RM], the results indicated that DNA sequence-dependent conformational changes alter the efficiency with which the repressor activates P[sub RM] transcription. These unexpected findings highlight the importance of the structure of the repressor-DNA interface in activating transcription from P[sub RM].
ACCESSION #
5322853

 

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