Primary Structure and Developmental Expression of Zebrafish Sodium Channel Na[sub v]1.6 during Neurogenesis

Tsai, Chi-Wei; Tseng, Jih-Jen; Lin, Su-Chun; Chang, Chia-Yao; Wu, Jen-Leih; Horng, Jun-Fun; Tsay, Huey-Jen
May 2001
DNA & Cell Biology;May2001, Vol. 20 Issue 5, p249
Academic Journal
A zebrafish sodium channel cDNA encoding a 1949-amino acid polypeptide, Na[sub v]1.6, was isolated. Two transcripts were detected in zebrafish adult brain but not in cardiac or skeletal muscle. The RNase protection analysis confirmed the neural specificity of zebrafish Na[sub v]1.6 24 hours postfertilization (hpf) Na[sub v]1.6 was expressed in the trigeminal ganglion, anterior and posterior lateral line ganglia, rhombomeres, and Rohon-Beard neurons. This preferential localization suggests that Na[sub v]1.6 plays an important role in tactile sensitivity. The abundance of zebrafish Na[sub v] 1.6 mRNA in the central and peripheral nervous systems increased markedly between 48 and 72 hpf, during the maturation of the nervous system.


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