Molecular Cloning and Preliminary Functional Analysis of Two Novel Human KRAB Zinc Finger Proteins, HKr18 and HKr19
- Molecular cloning and characterization of a novel RING zinc-finger protein gene up-regulated under in vitro salt stress in cassava. Reis, Sávio; Souza Conceição Tavares, Liliane; Nazaré Monteiro Costa, Carinne; Brígida, Aílton; Souza, Cláudia // Molecular Biology Reports;Jun2012, Vol. 39 Issue 6, p6513
Cassava ( Manihot esculenta Crantz) is one of the world's most important food crops. It is cultivated mainly in developing countries of tropics, since its root is a major source of calories for low-income people due to its high productivity and resistance to many abiotic and biotic factors. A...
- Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum. Xiuhong Yang; Chao Sun; Yuanlei Hu; Zhongping Lin // Journal of Biosciences;Mar2008, Vol. 33 Issue 1, p103
A RING zinc finger ankyrin protein gene, designated AdZFP1, was isolated from drought-tolerant Artemisia desertorum Spreng by mRNA differential display and RACE. Its cDNA was 1723 bp and encoded a putative protein of 445 amino acids with a predicted molecular mass of 47.9 kDa and an isoelectric...
- Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning. Fujii, Wataru; Kano, Kiyoshi; Sugiura, Koji; Naito, Kunihiko // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1
Zinc finger nuclease (ZFN) is a useful tool for endogenous site-directed genome modification. The development of an easier, less expensive and repeatedly usable construction method for various sequences of ZFNs should contribute to the further widespread use of this technology. Here, we...
- Cortical and hippocampal expression of the gene encoding a new transcriptional regulating protein. Duguay, Y.; Lapointe, A.; Lavallée, B.; Hum, D.W.; Rivest, S. // Molecular Psychiatry;2002, Vol. 7 Issue 10, p1036
Presents a darkfield photomicrograph taken from a mouse brain slice dipped into NTB-2 emulsion milk. Exhibition of two zinc-fingers of the C[sub 2]H[sub 2] subtype; Description of the nuclear protein.
- Plzf regulates limb and axial skeletal patterning. Barna, Maria; Hawe, Nicola; Niswander, Lee; Pandolfi, Pier Paolo // Nature Genetics;Jun2000, Vol. 25 Issue 2, p166
The promyelocytic leukaemia zinc finger (Plzf) protein (encoded by the gene Zfp145) belongs to the POZ/zinc-finger family of transcription factors. Here we generate Zfp145[sup -/-] mice and show that Plzf is essential for patterning of the limb and axial skeleton. Plzf inactivation results in...
- Fingers reach for the genome. Ansari, Aseem Z. // Nature Biotechnology;Mar2003, Vol. 21 Issue 3, p242
Discusses two studies concerning the application of zinc fingers to functional genomics and drug-target validation. Problems of targeting a unique sequence upstream of a gene; Ability of the stitched zinc finger to discriminate among possible 3 bp sequences in a yeast cell.
- Erratum: Unexpected failure rates for modular assembly of engineered zinc fingers. Ramirez, Cherie L; Foley, Jonathan E; Wright, David A; Müller-Lerch, Felix; Rahman, Shamim H; Cornu, Tatjana I; Winfrey, Ronnie J; Sander, Jeffry D; Fu, Fengli; Townsend, Jeffrey A; Cathomen, Toni; Voytas, Daniel F; Joung, J Keith // Nature Methods;Jun2008, Vol. 5 Issue 6, p575
A correction to the article "Unexpected failure rates for modular assembly of engineered zinc fingers" that was published in the 2008 issue is presented.
- Acetylation inactivates the transcriptional repressor BCL6. Bereshchenko, Oksana R.; Gu, Wei; Dalla-Favera, Riccardo // Nature Genetics;Dec2002, Vol. 32 Issue 4, p606
The proto-oncogene BCL6 encodes a BTB/POZ-zinc finger transcriptional repressor that is necessary for germinal-center formation and has been implicated in the pathogenesis of B-cell lymphomas. Here we show that the co-activator p300 binds and acetylates BCL6 in vivo and inhibits its function....
- Vessel maneuvers: Zinc fingers promote angiogenesis. Pasqualini, Renata; Barbas, Carlos F.; Arap, Wadih // Nature Medicine;Dec2002, Vol. 8 Issue 12, p1353
Reports on research which suggests that zinc-finger transcription factors can be engineered to target the promotion of angiogenesis in mice. Reference to a study in this issue of 'Nature Medicine' by Rebar et al.; Conclusion that the study verifies the value of transcription factors as targets...