The Sp1 Transcription Factor Regulates Cell Type-Specific Transcription of MUC1

Morris, Joanna R.; Taylor-Papadimitriou, Joyce
March 2001
DNA & Cell Biology;Mar2001, Vol. 20 Issue 3, p133
Academic Journal
Because the MUC1 mucin is highly expressed in breast and other carcinomas, interest is focused on the MUC1 promoter, particularly in the context of the delivery of genes to carcinomas. Earlier in vitro studies showed that the region between -152 and -66 of the MUC1 promoter is required for transcriptional activity in MUC1-expressing cells. Experiments reported here showed that sequences -119/-62 within this region are able to modulate transcription of the heterologous constitutively active herpes simplex virus thymidine kinase promoter in a pattern consistent with MUC1 expression. Band-shift experiments showed that although several factors (including Sp1 and Sp3) bind to these sequences, the element important in directing this MUC1 pattern of expression was an Sp1 GC box at -97. The data also show that the positioning or phase of the GC box was crucial for directing expression. The importance of the Sp1 transcription factor was confirmed by demonstrating that overexpression of Sp1 in MUC1-nonexpressing cells increased, not only the expression of a reporter gene driven by the 1.4-kb MUC1 promoter, but also the expression of MUC1 from the endogenous gene. Together, these data define an important role for Sp1 in the cell type-specific transcription of MUC1.


Related Articles

  • Genetic and functional analysis of the von Hippel-Lindau (VHL) tumour suppressor gene producer. Zatyka, M.; Morrissey, C.; Kuzmin, I.; Lerman, M.I.; Latif, F.; Richards, F.M.; Maher, E.R. // Journal of Medical Genetics;Jul2002, Vol. 39 Issue 7, p463 

    The VHL gatekeeper tumour suppressor gene is inactivated in the familial cancer syndrome von Hippel-Lindau disease and in most sporadic clear cell renal cell carcinomas. Recently the VHL gene product has been identified as a specific component of a SCF-like complex, which regulates proteolytic...

  • Profiling epigenetic inactivation of tumor suppressor genes in tumors and plasma from cutaneous melanoma patients. Hoon, Dave SB; Spugnardi, Mia; Kuo, Christine; Huang, Sharon K; Morton, Donald L; Taback, Bret // Oncogene;5/13/2004, Vol. 23 Issue 22, p4014 

    Aberrant methylation of CpG islands in promoter regions of tumor suppressor genes (TSG) has been demonstrated in epithelial origin tumors. However, the methylation profiling of tumor-related gene promoter regions in cutaneous melanoma tumors has not been reported. Seven known or candidate TSGs...

  • Microarray analysis of promoter methylation in lung cancers. Fukasawa, Masayuki; Kimura, Mika; Morita, Sumiyo; Matsubara, Kenichi; Yamanaka, Sumitaka; Endo, Chiaki; Sakurada, Akira; Sato, Masami; Kondo, Takashi; Horii, Akira; Sasaki, Hiroyuki; Hatada, Izuho // Journal of Human Genetics;Apr2006, Vol. 51 Issue 4, p368 

    Aberrant DNA methylation is an important event in carcinogenesis. Of the various regions of a gene that can be methylated in cancers, the promoter is the most important for the regulation of gene expression. Here, we describe a microarray analysis of DNA methylation in the promoter regions of...

  • Methylation of the CDH1 promoter as the second genetic hit in hereditary diffuse gastric cancer. Grady, William M.; Willis, Joseph; Guilford, Parry J.; Dunbier, Anita K.; Toro, Tumi T.; Lynch, Henry; Wiesner, Georgia; Ferguson, Kelly; Eng, Charis; Park, Jae-Gahb; Kim, Seong-Jin; Markowitz, Sanford // Nature Genetics;Sep2000, Vol. 26 Issue 1, p16 

    Aberrant promoter methylation and the associated loss of gene expression is a common accompaniment of human cancers. Nonetheless, it has been challenging to demonstrate in any given tumour that methylation of a specific gene was causal and not consequent to malignant transformation. In this...

  • Methylation of DLEC1 Promoter Is a Predictor for Recurrence in Chinese Patients with Gastric Cancer. Xiaobing Ye; Gang Feng; Nanlin Jiao; Chun Pu; Guohai Zhao; Guoping Sun // Disease Markers;2014, p1 

    Purpose. To investigate promoter methylation in the deleted in lung and esophageal cancer 1 (DLEC1) gene in Chinese patients with gastric cancer. Methods. A total of 227 patients with gastric cancer were enrolled. The methylations of the promoter regions of DLEC1 and ACTB were determined using...

  • Genetic diagnosis strategy of hereditary non-polyposis colorectal cancer. Jian-Qiu Sheng; Hong Zhang; Min Ji; Lei Fu; Hong Mu; Ming-Zhi Zhang; Ji-Sheng Huang; Min Han; Ai-Qin Li; Zhi Wei; Zi-Qin Sun; Zi-Tao Wu; Chang-Hong Xia; Shi-Rong Li; Hiyama, Toru // World Journal of Gastroenterology;2/28/2009, Vol. 15 Issue 8, p983 

    AIM: To study the characteristics of mismatch repair gene mutation of Chinese hereditary non-polyposis colorectal cancer (HNPCC) and hMLH1 gene promoter methylation, and to improve the screening strategy and explore the pertinent test methods. METHODS: A systematic analysis of 30 probands from...

  • Table of Contents.  // Carcinogenesis;Dec2011, Vol. 32 Issue 12, pNP 

    No abstract available.

  • MG624, an α7-nAChR antagonist, inhibits angiogenesis via the Egr-1/FGF2 pathway. Brown, Kathleen; Lau, Jamie; Dom, Aaron; Witte, Theodore; Luo, Haitao; Crabtree, Clayton; Shah, Yashoni; Shiflett, Brandon; Marcelo, Aileen; Proper, Nancy; Hardman, W.; Egleton, Richard; Chen, Yi; Mangiarua, Elsa; Dasgupta, Piyali // Angiogenesis;Mar2012, Vol. 15 Issue 1, p99 

    Small cell lung cancer (SCLC) demonstrates a strong etiological association with smoking. Although cigarette smoke is a mixture of about 4,000 compounds, nicotine is the addictive component of cigarette smoke. Several convergent studies have shown that nicotine promotes angiogenesis in lung...

  • ERG Cooperates with Androgen Receptor in Regulating Trefoil Factor 3 in Prostate Cancer Disease Progression. Rickman, David S.; Ying-bei Chen; Banerjee, Samprit; Yihang Pan; Jindan Yu; Vuong, Terry; Perner, Sven; Lafargue, Christopher J.; Mertz, Kirsten D.; Setlur, Sunita R.; Sircar, Kanishka; Chinnaiyan, Arul M.; Bismar, Tarek A.; Rubin, Mark A.; Demichelis, Francesca // Neoplasia;Dec2010, Vol. 12 Issue 12, p1031 

    To elucidate the role of ETS gene fusions in castration-resistant prostate cancer (CRPC), we characterized the transcriptome of 54 CRPC tumor samples from men with locally advanced or metastatic disease. Trefoil factor 3 (TFF3) emerged as the most highly differentially regulated gene with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics