Molecular Characterization of Murine Pancreatic Phospholipase A[sub 2]

Mandal, Asim K.; Zhang, Zhongjian; Chou, Janice Y.; Zimonjic, Drazen; Keck, Catherine L.; Popescu, Nicolae; Mukherjee, Anil B.
March 2001
DNA & Cell Biology;Mar2001, Vol. 20 Issue 3, p149
Academic Journal
The pancreatic secretory phospholipase A[sub 2] (sPLA[sub 2]IB) is considered to be a digestive enzyme, although it has several important receptor-mediated functions. In this study, using the newly isolated murine sPLA[sub 2]IB cDNA clone as a probe, we demonstrate that in addition to the pancreas, the sPLA[sub 2]IB mRNA was expressed in extrapancreatic organs such as the liver, spleen, duodenum, colon, and lungs. We also demonstrate that sPLA[sub 2]IB mRNA expression was detectable from the 17[sup th] day of gestation in the developing mouse fetus, coinciding with the time of completion of differentiation of the pancreas. Furthermore, the mRNA expression pattern of sPLA[sub 2]IB was distinct from those of sPLA[sub 2]IIA and cPLA[sub 2] in various tissues examined. The murine sPLA[sub 2]IB gene structure is well conserved, consistent with findings in other mammalian species, and this gene mapped to the region of mouse chromosome 5F1-G1.1. Taken together, our results suggest that sPLA[sub 2]IB plays important roles both in the pancreas and in extrapancreatic tissues and that in the mouse, its expression is developmentally regulated.


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