TITLE

Identification of Domains Mediating Transcription Activation, Repression, and Inhibition in the Paired-Related Homeobox Protein, Prx2 (S8)

AUTHOR(S)
Norris, Russell A.; Kern, Michael J.
PUB. DATE
February 2001
SOURCE
DNA & Cell Biology;Feb2001, Vol. 20 Issue 2, p89
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Despite the growing information concerning the developmental importance of the Prx2 protein, the structural determinants of Prx2 function are poorly understood. To gain insight into the transcription regulatory regions of the Prx2 protein, we generated a series of truncation mutants. Both the Prx2 response element (PRE) and a portion of the tenascin promoter, a downstream target of Prx2, were used as reporters in transient transfection assays. This analysis showed that a conserved domain (PRX), found in both Prx1 and Prx2, activated transcription in NIH 3T3 cells. This PRX domain, as well as other functional regions of Prx2, demonstrated both cell-specific and promoter-dependent transcriptional regulation. A second important region, the OAR (aristaless) domain, which is conserved among 35 Paired-type homeodomain proteins, was observed to inhibit transcription. Deletion of this element resulted in a 20-fold increase of transcription from the tenascin reporter in NIH 3T3 cells but not in C2C12 cells. The OAR domain did not function as a repressor in chimeric fusions with the Gal4 DNA binding domain in either cell type, characterizing it as an inhibitor instead of a repressor. These results give insight into the function of the Prx2 transcription factor while establishing the framework for comparison with the two isoforms of Prx1.
ACCESSION #
5322781

 

Related Articles

  • Enhancer deletions of the SHOX gene as a frequent cause of short stature: the essential role of a 250 kb downstream regulatory domain.  // Journal of Medical Genetics;Dec2009, Vol. 46 Issue 12, p6 

    Background: Mutations and deletions of the homeobox transcription factor gene SHOX are known to cause short stature. The authors have analysed SHOX enhancer regions in a large cohort of short stature patients to study the importance of regulatory regions in developmentally relevant genes like...

  • HNF1B polymorphism influences the prognosis of endometrial cancer patients: a cohort study. Mandato, Vincenzo Dario; Farnetti, Enrico; Torricelli, Federica; Abrate, Martino; Casali, Bruno; Ciarlini, Gino; Pirillo, Debora; Gelli, Maria Carolina; Nicoli, Davide; Grassi, Mario; Sala, Giovanni Battista L. A.; Palomba, Stefano; LA Sala, Giovanni Battista // BMC Cancer;2015, Vol. 15 Issue 1, p1 

    Background: HNF1B (formerly known as TCF2) gene encodes for a transcription factor that regulates gene expression involved in normal mesodermal and endodermal developments. A close association between rs4430796 polymorphism of HNF1B gene and decreased endometrial cancer (EC) risk...

  • Homeobox Genes and Human Genetic Disorders. Yangu Zhao; Westphal, Heiner // Current Molecular Medicine;Feb2002, Vol. 2 Issue 1, p13 

    Homeobox genes encode transcriptional regulators of embryonic development. Many genetic disorders affecting multiple organ systems have been associated with a diverse array of mutations in one or another of at least 27 different members of this gene family. We briefly describe the affected genes...

  • Mutant alleles of the Drosophila trithorax gene produce common and unusual homeotic and other... Breen, Thomas R. // Genetics;May99, Vol. 152 Issue 1, p319 

    Focuses on the trithorax (trx) gene of the Drosophila which encodes chromosome-binding proteins required throughout embryogenesis and imaginal development for tissue- and cell-specific levels of genetic transcription. Two other homeotic genes encoded by trx; Specific developmental functions of...

  • Molecular genetic analysis of Drosophila ash2, a member of the trithorax group required for... Adamson, Amy L.; Shearn, Allen // Genetics;Oct96, Vol. 144 Issue 2, p621 

    Examines the ash2 gene, a member of the trithorax group of genes which maintains active transcription of homeotic selector genes, in Drosophila. Homeotic transformations from mutations in ash2 gene; Location of the gene; Phenotypes of ash2 mutants; Enhancement or suppression of nasobemia...

  • Aeconserved cluster of three PRD-class homeobox genes (homeobrain, rx and orthopedia) in the Cnidaria and Protostomia. Mazza, Maureen E.; Pang, Kevin; Reitzel, Adam M.; Martindale, Mark Q.; Finnerty, John R. // EvoDevo;2010, Vol. 1 Issue 1, p1 

    Background: Homeobox genes are a superclass of transcription factors with diverse developmental regulatory functions, which are found in plants, fungi and animals. In animals, several Antennapedia (ANTP)-class homeobox genes reside in extremely ancient gene clusters (for example, the Hox,...

  • The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide. Espinás, Maria Lluïsa; Canudas, Sulvia; Fantp, Laura; Pimpinelli, Sergio; Casanova, Jordi; Azorín, Fernando // EMBO Reports;Sep2000, Vol. 1 Issue 3, p253 

    SAP18, a polypeptide associated with the Sin3-HDAC co-repressor complex, was identified in a yeast two-hybrid screen as capable of interacting with the Drosophila GAGA factor. The interaction was confirmed in vitro by glutathione S-transferase pull-down assays using recombinant proteins and...

  • Renal recovery after injury: the role of Pax-2. Lindoso, Rafael S.; Verdoorn, Karine S.; Einicker-Lamas, Marcelo // Nephrology Dialysis Transplantation;Sep2009, Vol. 24 Issue 9, p2628 

    The article discusses research on the association between the transcription factor Pax-2 and renal recovery process following an injury. Particular focus is given on the mechanisms associated with the regulation of its expression. A discussion on the Pax, paired homeobox, gene family is offered....

  • Chromosomal Distribution of PcG Proteins during Drosophila Development. Nègre, Nicolas; Hennetin, Jérôme; Sun, Ling V.; Lavrov, Sergey; Bellis, Michel; White, Kevin P.; Cavalli, Giacomo // PLoS Biology;Jun2006, Vol. 4 Issue 6, pe170 

    Polycomb group (PcG) proteins are able to maintain the memory of silent transcriptional states of homeotic genes throughout development. In Drosophila, they form multimeric complexes that bind to specific DNA regulatory elements named PcG response elements (PREs). To date, few PREs have been...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics