Expression of the Thimet Oligopeptidase Gene is Regulated by Positively and Negatively Acting Elements

McCool, Suzan; Pierotti, Adrian R.
December 2000
DNA & Cell Biology;Dec2000, Vol. 19 Issue 12, p729
Academic Journal
Thimet oligopeptidase (TOP) is a thiol-dependent metallopeptidase, which can cleave and thereby modulate the activity of many neuropeptides. The enzyme is active in many endocrine tissues, including testis, brain, and pituitary. In rat, the richest source of TOP is the testes, with a specific activity fivefold that of brain. The mechanism whereby rat TOP expression is regulated at the transcriptional level has been examined by reporter gene assay and electromobility shift assays after isolation of 1020 bp of upstream sequence. Computer analysis predicts a number of potential transcription factor-binding sites, which were examined by deletion analysis and DNA-binding studies. The promoter or its deletion fragments were fused to luciferase reporter gene vectors and introduced into GH3 pituitary, COS-1 kidney, MAT-Lu prostate, and GC-2spd(ts) spermatid cells. Two regions of the promoter have been identified: a positively acting region (-901/-219) and a strong negatively acting region (-219/-102). Concomitantly, potential transcription factors interacting with the cis-acting elements of the promoter were studied by gel electromobility shift assays. This work has identified a number of transcription factor-binding sites. However, no differences in the binding behavior in the various cell lines was observed.


Related Articles

  • Regulation of the transcription factor GATA-1 at the gene and protein level. Tang, X. -B.; Liu, D. -P.; Liang, C. -C. // Cellular & Molecular Life Sciences;Dec2001, Vol. 58 Issue 14, p2008 

    GATA-1, an important hematopoietic transcription factor, plays a critical role in differentiation and maturation of erythroid and megakaryocytic cell lines. GATA-1 appears to serve as a factor for virtually all characterized erythroid and megakaryocytic-expressed genes. Thus, defining the...

  • Comprehensive Analysis of Glucan Elicitor-Regulated Gene Expression in Tobacco BY-2 Cells Reveals a Novel MYB Transcription Factor Involved in the Regulation of Phenylpropanoid Metabolism. Tomonori Shinya; Ivan Gális; Tomoko Narisawa; Mami Sasaki; Hiroo Fukuda; Hideaki Matsuoka; Mikako Saito; Ken Matsuoka // Plant & Cell Physiology;Oct2007, Vol. 48 Issue 10, p1404 

    We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A....

  • Overexpression of Kruppel-like factor 4 in the human colon cancer cell line RKO leads to reduced tumorigenecity. Dang, Duyen T; Chen, Xinming; Feng, Jing; Torbenson, Michael; Dang, Long H; Yang, Vincent W // Oncogene;5/29/2003, Vol. 22 Issue 22, p3424 

    Kruppel-like factor 4 (KLF4) is a zinc-finger-containing transcription factor, the expression of which is enriched in the postmitotic cells of the intestinal epithelium. KLF4 is a target gene of the tumor suppressor adenomatous polyposis coli (APC). We sought to determine the role of KLF4 in...

  • Gene regulation: Fine-tuned amplification in cells. Dang, Chi V. // Nature;7/24/2014, Vol. 511 Issue 7510, p417 

    The article focuses on studies on regulation of gene expression by transcription factor Myc by researcher A. Sabò and S. Walz. It mentions that Walz used human cell lines for experimentally manipulating Myc expression and Sabò used mouse cell line for Myc expression. It also mentions that...

  • c-Myc: Linking Transformation and Genomic Instability. Prochownik, Edward V. // Current Molecular Medicine;Sep2008, Vol. 8 Issue 6, p446 

    CMYC has long been known to be among the most frequently de-regulated oncogenes in human cancer. Only recently, however has a clear understanding begun to emerge of how it promotes transformation. Through its role as a transcription factor, c-Myc alters the expression of hundreds of target...

  • G-protein-coupled receptor participates in 20- hydroxyecdysone signaling on the plasma membrane. Mei-Juan Cai; Du-Juan Dong; Yu Wang; Peng-Cheng Liu; Wen Liu; Jin-Xing Wang; Xiao-Fan Zhao // Cell Communication & Signaling;2014, Vol. 12 Issue 1, p1 

    Background Animal steroid hormones are conventionally known to initiate signaling via a genomic pathway by binding to the nuclear receptors. The mechanism by which 20E initiates signaling via a nongenomic pathway is unclear. Results We illustrate that 20E triggered the nongenomic pathway through...

  • Scaling Down SAGE: from miniSAGE to microSAGE. Datson, N. A. // Current Pharmaceutical Biotechnology;Oct2008, Vol. 9 Issue 5, p351 

    Since Serial Analysis of Gene Expression (SAGE) was introduced more than a decade ago, it has been widely applied to characterise gene expression profiles in various tissues, cell types and cell lines of diverse origin including human, mouse, rat, yeast, plant and parasites. Throughout the past...

  • A Functional Enhancer of Keratin14 Is a Direct Transcriptional Target of ΔNp63. Romano, Rose-Anne; Birkaya, Barbara; Sinha, Satrajit // Journal of Investigative Dermatology;May2007, Vol. 127 Issue 5, p1175 

    Keratin14 (K14) is a prototypic marker of dividing basal keratinocytes where its gene is transcribed at high levels. Transcriptional regulation of K14 is governed by an evolutionarily conserved functional enhancer marked by DNase 1 hypersensitive sites present upstream of the gene. This enhancer...

  • Characterization of functional elements in the neurofibromatosis (NF1) proximal promoter region. Min-Xu Zou; Butcher, Darci T.; Sadikovic, Bekim; Groves, Timothy C.; Siu-Pok Yee; Rodenhiser, David I. // Oncogene;1/15/2004, Vol. 23 Issue 2, p330 

    An essential requirement to understand how genes contribute to genetic disease is the thorough knowledge of the transcriptional regulation of gene expression. Here, we have characterized transcription factor binding sites within the type 1 neurofibromatosis (NF1) proximal regulatory region, and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics