TITLE

Insulin-Like Growth Factor-Binding Protein-3 Induces Fetalization in Neonatal Rat Cardiomyocytes

AUTHOR(S)
Henson, Margaret; Damm, Deborah; Lam, Andrew; Garrard, Lisa J.; White, Tyler; Abraham, Judith A.; Schreiner, George F.; Stanton, Lawrence W.; Joly, Alison H.
PUB. DATE
December 2000
SOURCE
DNA & Cell Biology;Dec2000, Vol. 19 Issue 12, p757
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We employed cDNA microarrays representing 4000 distinct sequences to profile changes in gene expression in a rodent model of heart disease, namely, progression to heart failure after myocardial infarction. Differential gene expression in the left ventricle was examined at 4-week intervals over a 12-week period after coronary artery ligation in rats. Over this time course, insulin-like growth factor-binding protein-3 (IGFBP-3) was found to have a greater expression than in nondiseased tissues. We then employed quantitative real-time PCR to analyze gene expression in neonatal rat cardiac myocytes that had been treated with recombinantly expressed IGFBP-3 to examine a number of transcriptional responses designed to reflect the heart failure phenotype. The IGFBP-3 protein was shown to induce transcription of atrial natriuretic factor (ANF) and �-myosin heavy chain (B-MHC). Analysis of conditioned media taken from IGFBP-3-treated cardiac myocyte cultures demonstrated an increase in ANF protein as well as in protein synthesis, as determined by metabolic incorporation of a radiolabeled amino acid. However, transcriptional changes of troponin-1, endothelin-1, or angiotensin-II by IGFBP-3 were not observed.
ACCESSION #
5322766

 

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