TITLE

Developmental Dynamics of a Polyhomeotic-EGFP Fusion in Vivo

AUTHOR(S)
Netter, Sophie; Faucheux, Marianne; Th�odore, Laurent
PUB. DATE
August 2001
SOURCE
DNA & Cell Biology;Aug2001, Vol. 20 Issue 8, p483
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
polyhomeotic is a member of the Polycomb group of genes. The products of this group are chromatin-associated proteins that act together as multimeric complexes. These proteins are required for the maintenance of target gene repression in a permanent and heritable manner during development. In order to better understand the dynamics of their action during development, we generated transgenic flies expressing a polyhomeotic protein tagged with the enhanced green fluorescent protein. Here we show that this fusion protein (PH-EGFP) retains both the functional properties of the endogenous protein and its target specificity on polytene chromosomes. The distribution of the PH-EGFP protein is partly dependent on the presence of wildtype Polycomb protein, indicating that PH-EGFP behaves as does the wildtype PH protein. Therefore, the PH-EGFP chimera appears to be an appropriate reporter of PH protein distribution and a suitable tool for the study of Polycomb-group complex assembly in vivo. The subnuclear distribution of PH-EGFP is dynamic throughout development. In the interphase nucleus at the cellular blastoderm, a diffuse granular pattern is observed. From the early gastrula stage onward, a few brighter dots appear. As development progressed from germ band retraction through hatching of the larva, numerous discrete dots accumulate in the nucleus of epidermal cells. The increasing number of dots observed during development may indicate that PH-EGFP is recruited at different stages on different target sites, a result that is in good agreement with functional data previously reported.
ACCESSION #
5322760

 

Related Articles

  • A Founder Mutation of the BRCA1-Gene in Western Sweden. Einbeigi, Zakaria; Kindblom, Jeane; Kindblom, Lars-Gunnar; Martinsson, Tommy; Suurkula, Mart; Wahlström, Jan; Wallgren, Arne; Karlsson, Per // Disease Markers;1999, Vol. 15 Issue 1/3, p99 

    Presents the abstract of the article 'A Founder Mutation of the BRCA1-Gene in Western Sweden,' by Zakaria Einbeigi, Jeane Kindblom, Lars-Gunnar Kindblom, Tommy Martinsson, Mart Suurkula, Jan Wahlstr ö m, Arne Wallgren and Per Karlsson.

  • Human Dkk-1, a gene encoding a Wnt antagonist, responds to DNA damage and its overexpression sensitizes brain tumor cells to apoptosis following alkulation damage of DNA. Jiang Shou; Ali-Osman, Francis; Multani, Asha S.; Pathak, Sen; Fedi, Paolo; Srivenugopal, Kalkunte S. // Oncogene;1/31/2002, Vol. 21 Issue 6, p878 

    Presents information on a study that investigated the p53-related functions of the human Dkk-1 gene. Introduction to the Dkk-1 gene; Research materials and methods; Results and discussion on the study.

  • The Coalescent and Infinite-Site Model of a Small Multigene Family. Innan, Hideki // Genetics;Feb2003, Vol. 163 Issue 2, p803 

    The infinite-site model of a small multigene family with two duplicated genes is studied. The expectations of the amounts of nucleotide variation within and between two genes and linkage disequilibrium are obtained, and a coalescent-based method for simulating patterns of polymorphism in a small...

  • Expanding the clinical phenotype of COG6 deficiency. Haijes, Hanneke; Prinsen, Hubertus C. M. T.; Thiel, Christian; Koerner, Christian; Verhoeven-Duif, Nanda M.; van Hasselt, Peter M. // Journal of Medical Genetics;Jun2014, Vol. 51 Issue 6, p425 

    A letter to the editor is presented in response to a novel syndrome of hypohydrosis and intellectual disability.

  • Erratum to: Inferring interaction type in gene regulatory networks using co-expression data.  // Algorithms for Molecular Biology;Aug2015, Vol. 10 Issue 1, p1 

    A correction to the article "Inferring interaction type in gene regulatory networks using co-expression data," in a 2015 issue is presented.

  • Technology: SMRT move? Koch, Linda // Nature Reviews Genetics;Mar2014, Vol. 15 Issue 3, p146 

    The article presents a research that investigates the challenges associated in the high-throughput sequencing and combination of single-molecule, real-time (SMRT) sequencing.

  • Evolution: Gene age begets constraint. Jones, Bryony // Nature Reviews Genetics;Feb2015, Vol. 16 Issue 2, p68 

    The article highlights a study on gene expression which constraints increase over time, which may be the result of the integration of genes into gene networks and the functional cellular environment.

  • Media Watch: This week's top...  // Pulse;6/11/2008, Vol. 68 Issue 21, p10 

    The article offers information on genetic testing for tardiness of persons in Great Britain. It reveals that a swab taken from a person's check can pick up certain genes which can make a person wake up early or get up late. Such test picks up the genes that control the natural pattern of sleep...

  • Occurrence and mechanisms of amikacin resistance and its association with {beta}-lactamases in Pseudomonas aeruginosa: a Korean nationwide study. Ja-Young Kim; Yeon-Joon Park; Hi Jeong Kwon; Kyungja Han; Moon Won Kang; Gun-Jo Woo // Journal of Antimicrobial Chemotherapy (JAC);Sep2008, Vol. 62 Issue 3, p479 

    : Objectives We investigated the occurrence and mechanism of amikacin resistance and its association with various β-lactamase genes in Pseudomonas aeruginosa isolates. : Methods Of the total 250 consecutive, non-duplicated isolates of P. aeruginosa, 55 isolates showed amikacin resistance....

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics