TITLE

Significantly Increased Cortisol Secretion in Normal Adrenocortical Cells Transfected with K-ras Mutants Derived from Human Functional Adrenocortical Tumors

AUTHOR(S)
Hsu, Chin-Hsun; Yang, Yuan-Chieh; Lian, Shen-Tsuen; Lee, Su-Chen; Shin, Shyi-Jang; Tsai, Juei-Hsiung; Lin, Shiu-Ru
PUB. DATE
April 2001
SOURCE
DNA & Cell Biology;Apr2001, Vol. 20 Issue 4, p231
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Our previous study showed that the mutation hotspots of the K-ras proto-oncogene in human functional adrenocortical tumors are in codons 15, 16, 18, and 31, thus differing from the sites in other tumors. In addition, analyzing the K-Ras protein by a recombinant DNA technique showed that the activity of endogenic GTPase and the GTPase-activating protein (GAP)-binding ability were significantly decreased in patients with these tumors. The aim of this study was to understand whether those K-ras mutants, which were found only in human adrenocortical tumors, play an important role in these tumors. Thus, the mutant K-ras cDNA was constructed with mammalian expression vectors and transfected into normal adrenocortical cells. The amount of cortisol secreted by the transfected cells was 20 to 30 times that of normal cells. Furthermore, Northern blot analysis revealed that the expression of the three steroidogenesis-related genes P450[sub scc] (cholesterol side-chain cleavage enzyme), P450[sub C17] (17a-hydroxylase/17, 20-lyase), and P450[sub C21] (steroid 21-hydroxylase) gene increased in the transfected cells. The K-ras oncogene significantly increases cortisol secretion by normal adrenocortical cells.
ACCESSION #
5322746

 

Related Articles

  • Spontaneous Rupture of a Functioning Adrenocortical Carcinoma. Jin Ook Chung; Dong Hyeok Cho; Jae Hyuk Lee; Dong Deuk Kwon; Dong Jin Chung; Min Young Chung // Yonsei Medical Journal;11/1/2010, Vol. 51 Issue 6, p974 

    Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis, and it can be classified as either a functional or nonfunctional tumor. Affected patients usually present with abdominal pain or with symptoms related to the mass effect or hormonal activity of the tumor. Several cases of...

  • Adrenocortical carcinoma posing as a pheochromocytoma: a diagnostic dilemma. Jain, Sumita; Agarwal, Lakshman; Nadkarni, Shravan; Ameta, Atul; Goyal, Ashish; Kumar, Ranjan; Rao, Arjun; Gupta, Kamalkant // Journal of Surgical Case Reports;May2014, Vol. 2014 Issue 5, p1 

    The article presents a case report of an adrenocortical carcinoma (ACC) in a 45-year old female patient with clinical symptoms and signs of pheochromocytoma and investigations resulting from a diagnostic dilemma. It explains that ACC tumor is a malignancy arising from the adrenal cortex. It...

  • Virilization of a Female Infant by a Maternal Adrenocortical Carcinoma. Lilah Morris; Steven Park; Timothy Daskivich; Bernard Churchill; C. Rao; Zhenmin Lei; Dorothy Martinez; Michael Yeh // Endocrine Practice;Mar2011, Vol. 17 Issue 2, pe1 

    Objective: To describe a possible mechanism underlying the partial virilization of a 46, XX infant by a functional maternal adrenocortical carcinoma (ACC).Methods: We performed immunocytochemical staining of tumor sections for luteinizing hormone (LH)/human chorionic gonadotropin (hCG)...

  • Molecular Markers and the Pathogenesis of Adrenocortical Cancer. Soon, Patsy S. H.; McDonald, Kerrie L.; Robinson, Bruce G.; Sidhu, Stan B. // Oncologist;May2008, Vol. 13 Issue 5, p548 

    Adrenal tumors are common, with an estimated incidence of 7.3% in autopsy cases, while adrenocortical carcinomas (ACCs) are rare, with an estimated prevalence of 4-12 per million population. Because the prognoses for adrenocortical adenomas (ACAs) and ACCs are vastly different, it is important...

  • Effects of repeated cortisol administration on brain potential correlates of episodic memory retrieval. McAllister-Williams, R. H.; Rugg, M. D. // Psychopharmacology;2002, Vol. 160 Issue 1, p74 

    Abstract Rationale: Neuropsychological impairments in depressive illness may be secondary to hypercortisola-emia. Cortisol administration to healthy subjects impairs episodic memory, though how this is mediated is un-known. Objectives: To examine the effects of 1 week's administration of...

  • Corticosteroid-Induced Lymphopenia, Immunosuppression, and Body Defense. Craddock, Charles G. // Annals of Internal Medicine;Apr78, Vol. 88 Issue 4, p564 

    Suggests that the immunologic response of the body to self-antigens exposed by disease or trauma may be suppressed by corticosteroid to offset the likelihood of autoimmune attack. Relation of the susceptability of lymphocytes to cortisol effects on cell numbers and body distribution; Details on...

  • Adrenal Cortical Adenoma and Adrenal Medullary Hyperplasia of the Right Adrenal Gland -- A Case Report. Borrero, Edgar; Katz, Paul; Upper, Stanley; Chang, John B. // Angiology;Mar1987, Vol. 38 Issue 3, p271 

    The normal adrenal gland contains two types of tissue, the adrenal cortical tissue and the adrenal medullary tissue. The cortex is divided into three portions: the outermost �zona glomerulosa� the central �zona fasciculate� and the innermost �zona recticularis.� The...

  • Effects of dexamethasone on neuroendocrine and psychological responses to L-tryptophan infusion. Porter, Richard J.; McAllister-Williams, R. Hamish; Jones, Samantha; Young, A. H. // Psychopharmacology;1999, Vol. 143 Issue 1, p64 

    Abstract 5-Hydroxytryptamine[sub 1A] (5-HT[sub 1A]) receptors have been shown to be suppressed by corticosteroid hormones in a variety of animal experimental paradigms. This effect may be central to the pathophysiology of severe clinical depressive illness, a condition in which 5-HT[sub 1A]...

  • Over the counter sale of topical corticosteroids: the need for debate. Shuster, Sam // British Medical Journal (Clinical Research Edition);7/6/1985, Vol. 291 Issue 6487, p38 

    Debates on the over the counter (OTC) sale of topical corticosteroids. Case of OTC hydrocortisone; Arguments and counterarguments; Misuse by patients on OTC hydrocortisone.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics