TITLE

Single sharp spot in fluorescence microscopy of two opposing lenses

AUTHOR(S)
Blanca, C. M.; Bewersdorf, J.; Hell, S. W.
PUB. DATE
October 2001
SOURCE
Applied Physics Letters;10/8/2001, Vol. 79 Issue 15, p2321
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We demonstrate theoretically, experimentally, and in an imaging application the possibility to generate a single predominant sharp diffraction maximum in the effective point-spread function of a fluorescence microscope that coherently uses two opposing lenses. This is achieved through binary pupil filters that preclude the origination of the unfavorable strong interference side maxima that are otherwise present in these systems. Mathematical postprocessing, which has so far been a prerequisite to gain artifact-free images, is now optional or obsolete. © 2001 American Institute of Physics.
ACCESSION #
5306387

 

Related Articles

  • High collection efficiency in flourescence microscopy with a solid immersion lens. Koyama, Kazuko; Yoshita, Masahiro // Applied Physics Letters;9/20/1999, Vol. 75 Issue 12, p1667 

    Analyzes the high efficiency of light collection in fluorescence microscopy using solid immersion lenses (SIL). Efficiency measurements using theories on radiation of dipoles near the surface of the dielectric medium; Kinds of SIL used; Determination of fluorescence intensity.

  • The merits of keeping protective filters on lenses. Younghein, Gaylord // PSA Journal;Mar95, Vol. 61 Issue 3, p16 

    Gives advice on the use of protective filters on camera lenses. Image quality; Necessity of using a lens hood in conjunction with protective filters; Care of lens.

  • THE EYES HAVE IT.  // Sea Magazine;Jul2011, Vol. 103 Issue 7, p38 

    The article discusses the significant advances made for lens designs and treatment on the sunglasses, and its effectiveness on the bearer. Sunglasses with adjustable gaskets create a seal around the eyes, suitable for sports such as waterskiing and wakeboarding. Sunglasses could be polarized to...

  • Focus on fringes. Dadwal, Namrata // Money Today;1/22/2009, p25 

    The article offers tips on choosing accessories for a camera. A wide-angle lens is recommended for shooting panoramas, while a telephoto lens may be used for shooting portraits. Using a natural density filter for avoiding the harsh glare of sunlight is suggested, while a polarising one will...

  • LETTERS. Holusha, G.; Henchy, Tom; Canup, Robert; Vargas, Bill; Wright, Ed // Popular Photography;Aug2010, Vol. 74 Issue 8, p26 

    Several letters to the editor are presented in response to articles in previous issues including "Invisible World," "Time Machine," both in the June 2010 issue, and another on the replacement of star lens designs in digital devices.

  • Camera Filters Part I. Tiffen, Ira // Image Technology;Oct1992, Vol. 74 Issue 8, p154 

    The article discusses the optical characteristics and applications of various types of camera filters. Camera filters are translucent optical element that changes the properties of light when entering the camera lens for enhancing the image. Filters have the ability to affect contrast,...

  • Refining Imagery: A Filter Buyer's Guide. MONTGOMERY, MARK // Videomaker;Mar2009, Vol. 23 Issue 9, p28 

    The article discusses the features of a good camera lens filter. The entry notes that glass filters are more superior than plastic in allowing more light to pass through the lens, while plastic filters are less expensive and more durable. In terms of shape, square filters are more preferred by...

  • Photo Travel. Gorrill, Robert B. // PSA Journal;Jun2005, Vol. 71 Issue 6, p41 

    Focuses on the factors that need to be considered when participating in photo travelling competitions. Importance of ensuring that there is no fading of filter colors; Use of lens hood to prevent extraneous light from striking the front surface of the lens that could cause loss of quality in...

  • Lenses in Focus. Bailey, Pat // Videomaker;Nov2005, Vol. 20 Issue 5, p36 

    The article presents information on camcorder lenses. Lenses are an important part of recording videos. They can help in ensuring that clear shots can be taken while out on the field. Lenses can alter colors, brightness, contrast or create various visual effects. There are many accessories which...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics