TITLE

Optimization of the Nutrient Supplients for Cellulase Production with the Basal Medium Palm Oil Mill Effluent

AUTHOR(S)
Rashid, S. S.; Alam, M. Z.; Karim, M. I. A.; Salleh, M. H.
PUB. DATE
December 2009
SOURCE
World Academy of Science, Engineering & Technology;Dec2009, Issue 36, p809
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A statistical optimization was studied to design a media composition to produce optimum cellulolytic enzyme where palm oil mill effluent (POME) as a basal medium and filamentous fungus, Trichoderma reesei RUT-C30 were used in the liquid state bioconversion(LSB). 2% (w/v) total suspended solid, TSS, of the POME supplemented with 1% (w/v) cellulose, 0.5%(w/v) peptone and 0.02% (v/v) Tween 80 was estimated to produce the optimum CMCase activity of 18.53 U/ml through the statistical analysis followed by the faced centered central composite design(FCCCD). The probability values of cellulose (<0.0011) and peptone (0.0021) indicated the significant effect on the production of cellulase with the determination coefficient (R²) of 0.995.
ACCESSION #
52941376

 

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