Optimization of the Nutrient Supplients for Cellulase Production with the Basal Medium Palm Oil Mill Effluent

Rashid, S. S.; Alam, M. Z.; Karim, M. I. A.; Salleh, M. H.
December 2009
World Academy of Science, Engineering & Technology;Dec2009, Issue 36, p809
Academic Journal
A statistical optimization was studied to design a media composition to produce optimum cellulolytic enzyme where palm oil mill effluent (POME) as a basal medium and filamentous fungus, Trichoderma reesei RUT-C30 were used in the liquid state bioconversion(LSB). 2% (w/v) total suspended solid, TSS, of the POME supplemented with 1% (w/v) cellulose, 0.5%(w/v) peptone and 0.02% (v/v) Tween 80 was estimated to produce the optimum CMCase activity of 18.53 U/ml through the statistical analysis followed by the faced centered central composite design(FCCCD). The probability values of cellulose (<0.0011) and peptone (0.0021) indicated the significant effect on the production of cellulase with the determination coefficient (R²) of 0.995.


Related Articles

  • Isolation and Characterisation of Cellobiohydrolase Gene from Aspergillus terreus SUK-1. Sidik, Nik Marzuki; Nasir, Siti Nurmi; Rusiman, Siti Nurbayan; Sharifuddin, Shaiful Adzni // Journal of Applied Sciences Research;Aug2011, Vol. 7 Issue 8, p1562 

    Aspergillus terreus SUK-1 isolated from oil palm waste was reported to produce cellulolytic enzymes for the degradation of cellulosic materials. RNA Ligase Mediated Rapid Amplification of cDNA Ends methodology was used to facilitate the isolation and cloning of a full length cDNA. The cDNA...

  • Ligno-Cellulose Composting: Case Study on Monitoring Oil Palm Residuals. Sal�tes, Sloan; Siregar, Fahri Arief; Caliman, Jean-Pierre; Liwang, Tony // Compost Science & Utilization;Autumn2004, Vol. 12 Issue 4, p372 

    Oil palm empty fruit bunches (EFB) composting is increasingly being considered by tropical farmers as a worthwhile alternative to direct spreading in the field. EFB are ligno-cellulose residues comprising 46% cellulose and 16.5% lignin with a high C:N ratio ranging from 45 to 70. In hot regions,...

  • EFFECTS OF PREHYDROLYSIS ON THE PRODUCTION OF DISSOLVING PULP FROM EMPTY FRUIT BUNCHES. Rosli, W.D. Wan; Leh, C.P.; Zainuddin, Z.; Tanaka, R. // Journal of Tropical Forest Science;July2004, Vol. 16 Issue 3, p343 

    The necessity of expanding and diversifying value-added features of oil palm lignocellulosic waste in Malaysia has prompted an examination of its suitability as a raw material for dissolving pulp production. Introduction of prehydrolysis prior to any alkaline pulping process helps to decrease...

  • Characteristics of cellulose nanofibers isolated from rubberwood and empty fruit bunches of oil palm using chemo-mechanical process. Jonoobi, Mehdi; Khazaeian, Abolghasem; Tahir, Paridah Md.; Azry, Syeed Saiful; Oksman, Kristiina // Cellulose;Aug2011, Vol. 18 Issue 4, p1085 

    In the present study, chemical-physical properties of nanofibers isolated from rubberwood ( Hevea brasiliensis) and empty fruit bunches (EFB) of oil palm ( Elaeis guineensis) were analyzed by microscopic, spectroscopic, thermal and X-ray diffraction methods. The isolation was achieved using...

  • Isolation, preparation, and characterization of nanofibers from oil palm empty-fruit-bunch (OPEFB). Fahma, Farah; Iwamoto, Shinichiro; Hori, Naruhito; Iwata, Tadahisa; Takemura, Akio // Cellulose;Oct2010, Vol. 17 Issue 5, p977 

    Cellulose nanofibers were produced by hydrolyzing oil palm empty-fruit-bunches with sulfuric acid. The effect of hydrolysis time on the structure and properties of the nanofibers was investigated. Fourier transform infrared spectroscopy was employed to evaluate the change of chemical...

  • Effect of Cooking Temperature on the Crystallinity of Acid Hydrolysed-oil Palm Cellulose. Ahmad Kuthi, Fatin Afifah Binti; Badri, Khairiah Haji // AIP Conference Proceedings;2014, Vol. 1614, p456 

    In this research, we studied the effect of acid hydrolysis temperature on the crystallinity of cellulose produced from empty fruit bunch (EFB). The hydrolysis temperature was studied from 120 to 140 °C at a fixed time and sulfuric acid, H2SO4 concentration which were 1 h and 1% (v/v)...

  • Hydrothermal Degradation of Enzymatic Hydrolysis Lignin in Water-Isopropyl Alcohol Co-Solvent. Zhuoming Ma; Shujun Li; Wei Qiao; Shixue Ren // BioResources;2016, Vol. 11 Issue 3, p6867 

    The effect of hydrothermal conditions on enzymatic hydrolysis lignin (EHL) degradation in water-isopropyl alcohol co-solvent and optimal conditions were investigated. The yields and reactivity toward formaldehyde of degraded enzymatic hydrolysis lignin (DEL) were determined. The optimal...

  • Optimal Length Transportation Hypothesis to Model Proteasome Product Size Distribution. Zaikin, Alexey; Kurths, Juergen // Journal of Biological Physics;Jul2006, Vol. 32 Issue 3/4, p231 

    This paper discusses translocation features of the 20S proteasome in order to explain typical proteasome length distributions. We assume that the protein transport depends significantly on the fragment length with some optimal length which is transported most efficiently. By means of a simple...

  • Solution and Level Identification of Sudoku Using Harmony Search. Nath Mandal, Satyendra; Sadhu, Saumi // International Journal of Modern Education & Computer Science;Apr2013, Vol. 5 Issue 3, p49 

    Different optimization techniques have been used to solve Sudoku. Zong Woo Geem have applied harmony search in Sudoku to get better result. He has taken a Sudoku and time complexity has been optimized by different values of parameters. But, he has not given way of solution in details. He has...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics