TITLE

Tail-Suspended Mice Lacking Calponin H1 Experience Decreased Bone Loss

AUTHOR(S)
Yotsumoto, Naoki; Takeoka, Michiko; Yokoyama, Minesuke
PUB. DATE
July 2010
SOURCE
Tohoku Journal of Experimental Medicine;Jul2010, Vol. 221 Issue 3, p221
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Calponin h1 (CNh1) is an actin-binding protein originally isolated from vascular smooth muscle and has been reported to suppress bone formation. We are therefore curious how CNh1 is involved in bone loss that is caused by space flight in microgravity. We assessed the effects of tail suspension (TS) in C57BL/6J wild (CN+/+) and CNh1-deleted (CN-/-) mice to elucidate the role of CNh1 in bone loss under weightless conditions. Bone mineral density (BMD) of tibiae was measured by single energy X-ray absorptiometry, and bone volume fraction (BV/TV), mineral apposition rate (MAR), and bone formation rate (BFR/BS) were measured by bone histomorphometry. BMD, BV/TV, MAR, and BFR/BS were lower in CN+/+ mice with TS than in those without. In the CN-/- group, however, the decrease in each of these parameters by TS was ameliorated. Decreases in serum osteocalcin levels by TS in CN+/+ mice were attenuated in CN-/- mice. Furthermore, urinary deoxypyridinolin (DPD), an indicator of bone resorption, was increased in CN+/+ mice following TS, but not in CN-/- mice. In transfection experiments, the degree of induction of bone formation markers, alkaline phosphatase (ALP) activity and bone morphogenetic protein (BMP)-4 mRNA expression, under stimulation with BMP-2, was lower in MC3T3-E1 mouse osteoblast-like cells expressing CNh1 than that in mock transfected cells. Notably, the BMP-2-induced ALP activity was decreased by CNh1 expression, which was partially rescued by treatment with the Rho kinase inhibitor Y27632. Taken together, these results indicate that CNh1 is responsible for weightlessness-induced bone loss in part through Rho signaling pathway.
ACCESSION #
52918018

 

Related Articles

  • Effect of Water-Soluble Silicon Supplementation on Bone Status and Balance of Calcium and Magnesium in Male Mice. Kim, Mi-Hyun; Kim, Eun-Jin; Jung, Ji-Youn; Choi, Mi-Kyeong // Biological Trace Element Research;May2014, Vol. 158 Issue 2, p238 

    Silicon (Si) is important for the growth and development of bone and connective tissues. Several studies have reported that Si supplementation improved bone mineral density (BMD) in female ovarectomized rats. However, few studies have investigated the effects of Si supplementation on bone status...

  • The Establishment of a Primary Culture System of Proximal Tubule Segments Using Specific Markers from Normal Mouse Kidneys. Kamiyama, Masumi; Garner, Michelle K.; Farragut, Kristina M.; Kobori, Hiroyuki // International Journal of Molecular Sciences;Apr2012, Vol. 13 Issue 4, p5098 

    The proximal tubule contains the highest expression of angiotensinogen mRNA and protein within the kidney and plays a vital role in the renal renin-angiotensin system. To study the regulation of angiotensinogen expression in the kidney in more detail, the proximal tubule needs to be accurately...

  • Computer-Automated Static, Dynamic and Cellular Bone Histomorphometry. Seung-Hyun Hong; Xi Jiang; Li Chen; Josh, Pujan; Dong-Guk Shin; Rowe, David // Journal of Tissue Science & Engineering;Special2012, Vol. 3 Issue S, Special section p1 

    Dynamic and cellular histomorphometry of trabeculae is the most biologically relevant way of assessing steady state bone health. Traditional measurement involves manual visual feature identification by a trained and qualified professional. Inherent with this methodology is the time and cost...

  • High phosphate feeding promotes mineral and bone abnormalities in mice with chronic kidney disease. Lau, Wei Ling; Linnes, Michael; Chu, Emily Y.; Foster, Brian L.; Bartley, Bryan A.; Somerman, Martha J.; Giachelli, Cecilia M. // Nephrology Dialysis Transplantation;Jan2013, Vol. 28 Issue 1, p62 

    Background Chronic kidney disease-mineral bone disorder (CKD-MBD) is a systemic syndrome characterized by imbalances in mineral homeostasis, renal osteodystrophy (ROD) and ectopic calcification. The mechanisms underlying this syndrome in individuals with chronic kidney disease (CKD) are not yet...

  • Stability of two β-tropomyosin isoforms: effects of mutation Arg91Gly. Charles Redwood; Dmitrii Levitsky // Journal of Muscle Research & Cell Motility;Dec2008, Vol. 29 Issue 6-8, p173 

    Abstract  In order to comprehend the domain structure of two β-tropomyosin (β-Tm) isoforms (skeletal muscle and smooth muscle β-Tm) and the influence of the disease-causing mutation Arg91Gly on it, we studied the thermal unfolding of these tropomyosin species by means of...

  • New DNA enzyme targeting Egr-1 mRNA inhibits vascular smooth muscle proliferation and regrowth after injury. Santiago, F.S; Lowe, H.C.; Kavurma, M.M.; Chesterman, C.N.; Baker, A.; Atkins, D.G.; Khachigian, L.M. // Nature Medicine;Dec99, Vol. 5 Issue 12, p1438 

    Presents a corrected reprint of the article 'New DNA enzyme targeting Egr-1 mRNA inhibits vascular smooth muscle proliferation and regrowth after injury,' by F.S. Santiago et.al. Errors; Corrections.

  • Ecto-5′-nucleotidase/CD73 knockdown increases cell migration and mRNA level of collagen I in a hepatic stellate cell line. Andrade, Cláudia; Lopez, Patrícia; Noronha, Bruno; Wink, Márcia; Borojevic, Radovan; Margis, Rogério; Lenz, Guido; Battastini, Ana; Guma, Fátima // Cell & Tissue Research;May2011, Vol. 344 Issue 2, p279 

    Ecto-5′-nucleotidase (eNT/CD73, E.C.3.1.3.5) is a glycosyl phosphatidylinositol (GPI)-linked cell-surface protein with several functions, including the local generation of adenosine from AMP, with the consequent activation of adenosine receptors and the salvaging of extracellular...

  • Constitutive Stimulation of Vascular Smooth Muscle Cells by Angiotensin II Derived From an Adenovirus Encoding a Furin-Cleavable Fusion Protein. Takayanagi, Takehiko; Bourne, Allison M.; Kimura, Keita; Takaguri, Akira; Elliott, Katherine J.; Eguchi, Kunie; Eguchi, Satoru // American Journal of Hypertension;Mar2012, Vol. 25 Issue 3, p280 

    BackgroundTo fill the gap between acute and chronic stimulation methods of angiotensin II (Ang II) and obtain relevant signaling information, we have made an adenovirus vector encoding a furin-cleavable Ang II fusion protein.MethodsVascular smooth muscle cells (VSMCs) were infected with...

  • Changes in the Expression of Smooth Muscle Contractile Proteins in TNBS- and DSS-Induced Colitis in Mice. Alkahtani, Reem; Mahavadi, Sunila; Al-Shboul, Othman; Alsharari, Shakir; Grider, John; Murthy, Karnam // Inflammation;Dec2013, Vol. 36 Issue 6, p1304 

    Thin filament-associated proteins such as calponin, caldesmon, tropomyosin, and smoothelin are thought to regulate acto-myosin interaction and thus, muscle contraction. However, the effect of inflammation on the expression of thin filament-associated proteins is not known. The aim of the present...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics