TITLE

Ex vivo and in vivo diagnosis of C6 glioblastoma development by Raman spectroscopy coupled to a microprobe

AUTHOR(S)
Beljebbar, Abdelilah; Dukic, Sylvain; Amharref, Nadia; Manfait, Michel
PUB. DATE
September 2010
SOURCE
Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 1, p477
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The potential of Raman spectroscopy for ex vivo and in vivo classification of normal and glioblastoma brain tumor development was investigated. High-quality spectra of normal and tumor tissues were obtained using a portable Raman spectrometer coupled to a microprobe with a signal integration time of 5 s. Ex vivo results demonstrated that by using the biochemical information contained in the spectra, we were able to distinguish between normal brain features (white and gray matter), invasion, and tumor tissues with a classification accuracy of 100%. Differences between these features resulted from variations in their lipid signal contributions, which probably reflect differences in the level of myelinization. This finding supports the ability of in vivo Raman spectroscopy to delineate tumor margins during surgery. After implanting C6 cells in rat brain, we monitored, in vivo, the development of glioblastoma tumor from days 0 to 20 post-implantation (PI). The classification exhibited a clear separation of the data into two clusters: one cluster was associated with normal brain tissues (cortex), and the second was related to data measured from tumor evolution. The second cluster could be divided into two subclusters, one associated with tumor tissue from 4 to 13 days PI and the second related to tumor tissue from 15 to 20 days PI. Histological analysis reveals that the differences between these two subclusters are: the presence of a massive infiltration zone in the brain tissue from 4 to 13 days PI, and; a maturation of the tumor characterized by the appearance of edematous and necrotic zones, as well as a diminution in the proliferative and invasive area, from 15 days. This work demonstrates the potential of Raman spectroscopy to provide diagnostic information for the early detection of tumors in vivo.
ACCESSION #
52815225

 

Related Articles

  • Prediction of Glioblastoma Multiform Response to Bevacizumab Treatment Using Multi-Parametric MRI. Najafi, Mohammad; Soltanian-Zadeh, Hamid; Jafari-Khouzani, Kourosh; Scarpace, Lisa; Mikkelsen, Tom // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Glioblastoma multiform (GBM) is a highly malignant brain tumor. Bevacizumab is a recent therapy for stopping tumor growth and even shrinking tumor through inhibition of vascular development (angiogenesis). This paper presents a noninvasive approach based on image analysis of multi-parametric...

  • Vascular gene expression patterns are conserved in primary and metastatic brain tumors. Yang Liu; Carson-Walter, Eleanor B.; Cooper, Anna; Winans, Bethany N.; Johnson, Mahlon D.; Walter, Kevin A. // Journal of Neuro-Oncology;Aug2010, Vol. 99 Issue 1, p13 

    Malignant primary glial and secondary metastatic brain tumors represent distinct pathological entities. Nevertheless, both tumor types induce profound angiogenic responses in the host brain microvasculature that promote tumor growth. We hypothesized that primary and metastatic tumors induce...

  • Prediction of Glioblastoma Multiform Response to Bevacizumab Treatment Using Multi-Parametric MRI. Najafi, Mohammad; Soltanian-Zadeh, Hamid; Jafari-Khouzani, Kourosh; Scarpace, Lisa; Mikkelsen, Tom // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Glioblastoma multiform (GBM) is a highly malignant brain tumor. Bevacizumab is a recent therapy for stopping tumor growth and even shrinking tumor through inhibition of vascular development (angiogenesis). This paper presents a noninvasive approach based on image analysis of multi-parametric...

  • Outcome of conventional treatment and prognostic factor in elderly glioblastoma patients. Oh, Sung; Jee, Tae; Kong, Doo-Sik; Nam, Do-Hyun; Lee, Jung-Il; Seol, Ho // Acta Neurochirurgica;Apr2014, Vol. 156 Issue 4, p641 

    Background: Glioblastoma (GBM) is the most life-threatening primary brain tumour. Especially in elderly patients, a poorer outcome is noticeable. Until now, the effectiveness of the conventional active treatment has been controversial. The purpose of this study is to find the optimal treatment...

  • A Unique Four-Hub Protein Cluster Associates to Glioblastoma Progression. Simeone, Pasquale; Trerotola, Marco; Urbanella, Andrea; Lattanzio, Rossano; Ciavardelli, Domenico; Di Giuseppe, Fabrizio; Eleuterio, Enrica; Sulpizio, Marilisa; Eusebi, Vincenzo; Pession, Annalisa; Piantelli, Mauro; Alberti, Saverio // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    Gliomas are the most frequent brain tumors. Among them, glioblastomas are malignant and largely resistant to available treatments. Histopathology is the gold standard for classification and grading of brain tumors. However, brain tumor heterogeneity is remarkable and histopathology procedures...

  • NANOG regulates glioma stem cells and is essential in vivo acting in a cross-functional network with GLI1 and p53. Zbinden, Marie; Duquet, Arnaud; Lorente-Trigos, Aiala; Ngwabyt, Sandra-Nadia; Borges, Isabel; Altaba, Ariel Ruiz i // EMBO Journal;8/4/2010, Vol. 29 Issue 15, p2659 

    A cohort of genes associated with embryonic stem (ES) cell behaviour, including NANOG, are expressed in a number of human cancers. They form an ES-like signature we first described in glioblastoma multiforme (GBM), a highly invasive and incurable brain tumour. We have also shown that...

  • Chemokine receptor CXCR3 promotes growth of glioma. Liu, Che; Luo, Defang; Reynolds, Brent A.; Meher, Geeta; Katritzky, Alan R.; Lu, Bao; Gerard, Craig J.; Bhadha, Cyrus P.; Harrison, Jeffrey K. // Carcinogenesis;Feb2011, Vol. 32 Issue 2, p129 

    Human glioblastoma multiforme (GBM) is the most common primary brain tumor in adults. The poor prognosis and minimally successful treatments of GBM indicates a need to identify new therapeutic targets. In this study, we examined the role of CXCR3 in glioma progression using the GL261 murine...

  • Invasive Glioblastoma Cells Acquire Stemness and Increased Akt Activation. Molina, Jennifer R.; Hayashi, Yuho; Stephens, Clifton; Georgescu, Maria-Magdalena // Neoplasia;Jun2010, Vol. 12 Issue 6, p453 

    Glioblastoma multiforme (GBM) is the most frequent and most aggressive brain tumor in adults. The dismal prognosis is due to postsurgery recurrences arising from escaped invasive tumor cells. The signaling pathways activated in invasive cells are under investigation, and models are currently...

  • DTI and PWI analysis of peri-enhancing tumoral brain tissue in patients treated for glioblastoma. Stecco, Alessandro; Pisani, Carla; Quarta, Raffaella; Brambilla, Marco; Masini, Laura; Beldì, Debora; Zizzari, Sara; Fossaceca, Rita; Krengli, Marco; Carriero, Alessandro // Journal of Neuro-Oncology;Apr2011, Vol. 102 Issue 2, p261 

    To analyse the role of MR diffusion-tensor imaging (DTI) and perfusion-weighted imaging (PWI) in characterising tumour boundaries in patients with glioblastoma multiforme. Seventeen patients with surgically treated WHO IV grade gliomas who were candidates for adjuvant chemo-radiotherapy were...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics