Ionic liquid matrices for MALDI-TOF-MS analysis of intact glycoproteins

Giménez, Estela; Benavente, Fernando; Barbosa, José; Sanz-Nebot, Victoria
September 2010
Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 1, p357
Academic Journal
2,5-Dihydroxybenzoic acid (DHB) has been demonstrated to be a more suitable matrix than 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid, SA) to obtain reliable molecular mass values of intact glycoproteins because it prevents sugar fragmentation. Lack of spot homogeneity during the crystallization step was prevented by drying the sample-matrix mixture under vacuum conditions. Nevertheless, this sample-matrix preparation procedure requires a specific experimental setup and may be time-consuming. In this work, we investigated the effectiveness of different ionic liquid matrices (ILMs) with SA and DHB on the ionization of a set of intact glycoproteins with several degrees of glycosylation. The obtained results demonstrate that some of the tested ILMs allow detection of the studied intact glycoproteins. Furthermore, the selected optimum conditions solve the reproducibility issue of using the DHB as a solid matrix without the vacuum drying method and, surprisingly, avoid sugar fragmentation when both SA and DHB were used as ILMs.


Related Articles

  • Organophosphorus. Keglevich, Gy�rgy // Current Organic Chemistry;Jul2010, Vol. 14 Issue 12, p1170 

    The article discusses various reports published within the issue including one on the advantages of applying ionic liquids instead of traditional solvents and another on the activating ability of ionic liquids.

  • Enzyme Mediated Reactions in Ionic Liquids. Harjani, J. R.; Naik, P. U.; Nara, S. J.; Salunkhe, M. M. // Current Organic Synthesis;Oct2007, Vol. 4 Issue 4, p354 

    The virtues associated with use of ionic liquids for enzyme-mediated reactions make them promising alternatives to the conventional reaction media. The scope of this review includes the developments witnessed by the area of the biotransformations in ionic liquids over more than a decade,...

  • Editorial [Hot Topic:Synthesis in Ionic Liquids (Guest Editor: Kenneth K. Laali)]. Laali, Kenneth K. // Current Organic Synthesis;Oct2007, Vol. 4 Issue 4, p352 

    Application of ionic liquids (ILs) in synthesis continues to experience astonishing growth. The realization that physical and chemical properties of the ILs can be tailored by judicious selection of the cation and anion structures, has undoubtedly contributed to the enthusiasm by the synthetic...

  • Chemoenzymatic synthesis of feruloylated monoacyl- and diacyl-glycerols in ionic liquids. Shangde Sun; Guolong Yang; Yanlan Bi; Fugang Xiao // Biotechnology Letters;Dec2009, Vol. 31 Issue 12, p1885 

    Feruloylated monoacyl- and diacyl-glycerols (FMAGs and FDAGs) are lipophilic antioxidants and potential UV absorbers. FMAGs and FDAGs were synthesized by a novel chemoenzymatic method: firstly, ferulic acid was esterified with glycerol to synthesize glyceryl ferulate, using p-toluenesulfonic...

  • Computational study of proton and methyl cation affinities of imidazole-based highly energetic ionic liquids. Singh, Hari; Mukherjee, Uttama // Journal of Molecular Modeling;Oct2011, Vol. 17 Issue 10, p2687 

    The present study deals with the evaluation of gas phase proton and methyl cation affinities for alkyl- and nitrosubstituted imidazoles using DFT (B3LYP)/6-31 + G(d) and MP2 methods in the Gaussian 03 software package. The extent of charge delocalization of these cations is correlated with...

  • Importance of the Ionic Nature of Ionic Liquids in Affecting Enzyme Performance. Zhen Yang; Ya-Jun Yue; Wen-Chao Huang; Xiao-Ma Zhuang; Zheng-Tao Chen; Miao Xing // Journal of Biochemistry;Mar2009, Vol. 145 Issue 3, p355 

    The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF6], [BMIm][BF4]), and [BMIm][MeSO4], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO4, KPF6, and NaBF4) on the enzyme...

  • Hierarchical LiFePO4 with a controllable growth of the (010) facet for lithium-ion batteries. Binbin Guo; Hongcheng Ruan; Cheng Zheng; Hailong Fei; Mingdeng Wei // Scientific Reports;9/27/2013, p1 

    Hierarchically structured LiFePO4 was successfully synthesized by ionic liquid solvothermal method. These hierarchically structured LiFePO4 samples were constructed from nanostructured platelets with their (010) facets mainly exposed. To the best of our knowledge, facet control of a hierarchical...

  • A Simple Halide-to-Anion Exchange Method for Heteroaromatic Salts and Ionic Liquids. Alcalde, Ermitas; Dinarès, Immaculada; Ibáñez, Anna; Mesquida, Neus // Molecules;Apr2012, Vol. 17 Issue 4, p4007 

    A broad and simple method permitted halide ions in quaternary heteroaromatic and ammonium salts to be exchanged for a variety of anions using an anion exchange resin (A- form) in non-aqueous media. The anion loading of the AER (OH- form) was examined using two different anion sources, acids or...

  • Ionic Liquid Applications in Peptide Chemistry: Synthesis, Purification and Analytical Characterization Processes. Tietze, Alesia A.; Heimer, Pascal; Stark, Annegret; Imhof, Diana // Molecules;Apr2012, Vol. 17 Issue 4, p4158 

    This review aims to provide a comprehensive overview of the recent advances made in the field of ionic liquids in peptide chemistry and peptide analytics.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics