TITLE

Integration of plasma-assisted surface chemical modification, soft lithography, and protein surface activation for single-cell patterning

AUTHOR(S)
Cheng, Q.; Komvopoulos, K.
PUB. DATE
July 2010
SOURCE
Applied Physics Letters;7/26/2010, Vol. 97 Issue 4, p043705
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Surface patterning for single-cell culture was accomplished by combining plasma-assisted surface chemical modification, soft lithography, and protein-induced surface activation. Hydrophilic patterns were produced on Parylene C films deposited on glass substrates by oxygen plasma treatment through the windows of polydimethylsiloxane shadow masks. After incubation first with Pluronic F108 solution and then serum medium overnight, surface seeding with mesenchymal stem cells in serum medium resulted in single-cell patterning. The present method provides a means of surface patterning with direct implications in single-cell culture.
ACCESSION #
52616453

 

Related Articles

  • Biointerface: protein enhanced stem cells binding to implant surface. Chrzanowski, W.; Kondyurin, A.; Lee, Jae; Lord, Megan; Bilek, M.; Kim, Hae-Won // Journal of Materials Science: Materials in Medicine;Sep2012, Vol. 23 Issue 9, p2203 

    The number of metallic implantable devices placed every year is estimated at 3.7 million. This number has been steadily increasing over last decades at a rate of around 8 %. In spite of the many successes of the devices the implantation of biomaterial into tissues almost universally leads to the...

  • Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells. Tchao, Jason; Lu Han; Bo Lin; Lei Yang; Tobita, Kimimasa // Scientific Reports;10/17/2014, p1 

    Cellular cardiomyoplasty has emerged as a novel therapy to restore contractile function of injured failing myocardium. Human multipotentmuscle derived stem cells (MDSC) can be a potential abundant, autologous cell source for cardiac repair. However, robust conditions for cardiomyocyte (CM)...

  • Neural Epidermal Growth Factor-Like Like Protein 2 (NELL2) Promotes Aggregation of Embryonic Carcinoma P19 Cells by Inducing N-Cadherin Expression. Kim, Dong Hee; Kim, Han Rae; Choi, Eun Jung; Kim, Dong Yeol; Kim, Kwang Kon; Kim, Byung Sam; Park, Jeong Woo; Lee, Byung Ju // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    NELL2 was first identified as a mammalian homolog of chick NEL (Neural EGF-like) protein. It is almost exclusively expressed in neurons of the rat brain and has been suggested to play a role in neural differentiation. However, there is still no clear evidence for the detailed function of NELL2...

  • Mesenchymal Stem Cell Responses to Bone-Mimetic Electrospun Matrices Composed of Polycaprolactone, Collagen I and Nanoparticulate Hydroxyapatite. Phipps, Matthew C.; Clem, William C.; Catledge, Shane A.; Yuanyuan Xu; Hennessy, Kristin M.; Thomas, Vinoy; Jablonsky, Michael J.; Chowdhury, Shafiul; Stanishevsky, Andrei V.; Vohra, Yogesh K.; Bellis, Susan L. // PLoS ONE;2011, Vol. 6 Issue 2, p1 

    The performance of biomaterials designed for bone repair depends, in part, on the ability of the material to support the adhesion and survival of mesenchymal stem cells (MSCs). In this study, a nanofibrous bone-mimicking scaffold was electrospun from a mixture of polycaprolactone (PCL), collagen...

  • A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses. Stern, Eric; Mooney, David J.; Fahmy, Tarek M. // International Journal of Biomaterials;2009, Vol. 2009, Special section p1 

    Current efforts in surface functionalization have not produced a robust technique capable of creating specific two-dimensional microscale geometrical arrays composed of multiple proteins. Such a capability is desirable for engineering substrates in sensing and cell patterning applications where...

  • In Vitro Epigenetic Reprogramming of Human Cardiac Mesenchymal Stromal Cells into Functionally Competent Cardiovascular Precursors. Vecellio, Matteo; Meraviglia, Viviana; Nanni, Simona; Barbuti, Andrea; Scavone, Angela; DiFrancesco, Dario; Farsetti, Antonella; Pompilio, Giulio; Colombo, Gualtiero I.; Capogrossi, Maurizio C.; Gaetano, Carlo; Rossini, Alessandra // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    Adult human cardiac mesenchymal-like stromal cells (CStC) represent a relatively accessible cell type useful for therapy. In this light, their conversion into cardiovascular precursors represents a potential successful strategy for cardiac repair. The aim of the present work was to reprogram...

  • CD36 Participates in PrP106-126-Induced Activation of Microglia. Kouadir, Mohammed; Yang, Lifeng; Tan, Rongrong; Shi, Fushan; Lu, Yun; Zhang, Siming; Yin, Xiaomin; Zhou, Xiangmei; Zhao, Deming // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Tissue-specific stem cells are considered to have a limited differentiation potential. Recently, this notion was challenged by reports that showed a broader differentiation potential of neural stem cells, in vitro and in vivo, although the molecular mechanisms that regulate plasticity of neural...

  • CD36 Participates in PrP106-126-Induced Activation of Microglia. Kouadir, Mohammed; Yang, Lifeng; Tan, Rongrong; Shi, Fushan; Lu, Yun; Zhang, Siming; Yin, Xiaomin; Zhou, Xiangmei; Zhao, Deming // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Tissue-specific stem cells are considered to have a limited differentiation potential. Recently, this notion was challenged by reports that showed a broader differentiation potential of neural stem cells, in vitro and in vivo, although the molecular mechanisms that regulate plasticity of neural...

  • Measurement of C-reactive protein in adults with febrile neutropenia after hematopoietic cell transplantation. Rovira, M.; Almela, M.; de la Bellacasa, J. P.; Carreras, E.; Mensa, J.; Ortega, M. // Bone Marrow Transplantation;Apr2004, Vol. 33 Issue 7, p741 

    Summary:The aim of this study was to evaluate the usefulness of C-reactive protein (CRP) monitoring in the differential diagnosis and prognosis of febrile neutropenic episodes in hematopoietic cell transplantation (HCT). In all, 100 patients were enrolled in the study. The CRP was determined in...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics