TITLE

Comparison of Helicobacter pylori and Escherichia coli in induction of TNF-α mRNA from human peripheral blood mononuclear cells

AUTHOR(S)
Ahmadzadeh, E.; Zarkesh-Esfahani, H.; Roghanian, R.; Akbar, F. Navab
PUB. DATE
July 2010
SOURCE
Indian Journal of Medical Microbiology;Jul2010, Vol. 28 Issue 3, p233
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Purpose: To investigate the difference between the abilities of Helicobacter pylori and Escherichia coli to induce expression of TNF-α in human peripheral blood mononuclear cells (PBMC). Materials and Methods: H pylori was isolated from gastric biopsy specimens. The mononuclear cells were isolated from human blood, cultured, and treated with either intact or sonicated E coli or H pylori, and mRNA expression for TNF-α was detected using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: TNF-α mRNA expression levels were significantly higher in PBMCs stimulated with E coli compared to those stimulated with H pylori at the same number and identical conditions (P < .001). The results also suggest that sonicated bacteria were significantly (P < .001) less stimulatory for PBMCs than intact bacteria for both E coli and H pylori. Conclusions: The ability of different H pylori strains isolated from biopsy samples to stimulate TNF-α from PBMCs was significantly lower than that of E coli. Sonicated bacteria, as compared to intact bacteria, was a very poor inducer of TNF-α mRNA expression, suggesting that the conformation of lipopolysaccharides (LPS) on the outer leaflet of the outer membrane is not totally conserved in sonicated bacteria.
ACCESSION #
52597057

 

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