Kinetics of chemokine secretion in human macrophages infected with various strains of Mycobacterium tuberculosis

Rajavelu, P.; Das, S. D.
July 2010
Indian Journal of Medical Microbiology;Jul2010, Vol. 28 Issue 3, p201
Academic Journal
Background and Purpose: It has been shown that chemokine secretion upon infection with Mycobacterium tuberculosis is influenced by the virulence of the strain, and it is suggested that virulence-associated differences in chemokine secretion contribute to the failure in containing the infection due to poor granuloma formation. Materials and Methods: In this study, we used prevalent M tuberculosis clinical strains (S7 and S10) to study the chemokine secretion profile in infected THP-1 cells and monocyte-derived macrophages (MDM) and compared this with the chemokine secretion induced by laboratory strains. Results: This study showed that comparatively lower levels of IP-10 were induced by clinical strains than by laboratory strains in both differentiated THP-1 and MDMs. The secretion of MIP-1a was also depressed but only in the THP-1 cells infected with clinical strains. This depressed chemokine secretion may hinder the movement of Th-1 cells from the periphery into the infection foci to control the infection. Correlation between IP-10 and IL-12p40 showed a negative relationship in control MDMs, while there was a positive correlation in all the infected strains, indicating their cooperative role in attracting and activating Th1 cells for a protective immune response at the site. This relationship was strain dependent, with avirulent H37Ra showing higher correlation, followed by the clinical strains and the virulent H37Rv. A positive correlation of IP-10 with IFN-γ (S7 and H37Ra) and with IL-10 (H37Ra and H37Rv) suggested a definitive interplay of these molecules in infection. Conclusions: The chemokines secretion by infected THP-1 cells and MDMs was strain dependant and the lower induction by the clinical strains may indicate that the clinical strains maintain a quiescent nature to mislead the host immune system for their benefit.


Related Articles

  • Interleukin-12 and Interleukin-27 Regulate Macrophage Control of Mycobacterium tuberculosis. Robinson, Cory M.; Nau, Gerard J. // Journal of Infectious Diseases;8/1/2008, Vol. 198 Issue 3, p359 

    Mycobacterium tuberculosis is an intracellular pathogen that persists within macrophages and remains a considerable global threat to human health. The purpose of this study was to investigate how interleukin (IL)-12 and IL-27 regulate human macrophage interactions with M. tuberculosis....

  • IL10 and IL12B polymorphisms each influence IL-12p70 secretion by dendritic cells in response to LPS. Peng, Judy C.; Abu Bakar, Sazaly; Richardson, Michelle M.; Jonsson, Julie J.; Frazer, Ian H.; Nielsen, Lars K; Morahan, Grant; Thomas, Ranjeny // Immunology & Cell Biology;Apr2006, Vol. 84 Issue 2, p227 

    Dendritic cells (DC) are the main producers of the cytokine IL-12p70, through which they play a direct role in the development of IFN-γ-secreting Th1 cells, costimulation of CTL differentiation and NK-cell activation. In contrast, IL-10, which is also produced by DC, negatively regulates...

  • IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils- fibroblasts interaction in psoriasis. Glowacka, E.; Lewkowicz, P.; Rotsztejn, H.; Zalewska, A. // Advances in Medical Sciences (De Gruyter Open);2010, Vol. 55 Issue 2, p254 

    Purpose: Psoriasis is a common skin disease affecting about 1-3% of the world population. Many types of cells, including lymphocytes, dendritics APCs (antigen presenting cells), NKT (natural killer T) cells, neutrophils, keratinocytes and fibroblasts are involved in the pathogenesis of...

  • Regulatory Effect of SOCS on NF-κB Activity in Murine Monocytes/Macrophages. Park, Sun-Hee; Kim, Kyoung-Eun; Hwang, Ha-Young; Kim, Tae Yoon // DNA & Cell Biology;Feb2003, Vol. 22 Issue 2, p131 

    The suppressor of cytokine signaling (SOCS) group of proteins has been implicated in regulation of various cytokine signaling and in a negative crosstalk between distinct signaling pathways. Interleukin-10 (IL-10) and LPS were known to induce expression of SOCS-3 in neutrophils and...

  • Human Macrophage Responses to Clinical Isolates from the Mycobacterium tuberculosis Complex Discriminate between Ancient and Modern Lineages. Portevin, Damien; Gagneux, Sébastien; Comas, Iñaki; Young, Douglas // PLoS Pathogens;Mar2011, Vol. 7 Issue 3, preceding p1 

    No abstract available.

  • Cellular and Humoral Mechanisms Involved in the Control of Tuberculosis. Zuñiga, Joaquin; Torres-García, Diana; Santos-Mendoza, Teresa; Rodriguez-Reyna, Tatiana S.; Granados, Julio; Yunis, Edmond J. // Clinical & Developmental Immunology;2012, p1 

    Mycobacterium tuberculosis (Mtb) infection is a major international public health problem. One-third of the world's population is thought to have latent tuberculosis, a condition where individuals are infected by the intracellular bacteria without active disease but are at risk for reactivation,...

  • Protective Immunity to Mycobacterium tuberculosis Infection by Chemokine and Cytokine Conditioned CFP- 10 Differentiated Dendritic Cells. Salam, Nasir; Gupta, Shashank; Sharma, Sachin; Pahujani, Shweta; Sinha, Aprajita; Saxena, Rajiv K.; Natarajan, Krishnamurthy // PLoS ONE;2008, Vol. 3 Issue 8, p1 

    Background: Dendritic cells (DCs) play major roles in mediating immune responses to mycobacteria. A crucial aspect of this is the priming of T cells via chemokines and cytokines. In this study we investigated the roles of chemokines RANTES and IP- 10 in regulating protective responses from...

  • Modulation of Cytokines and Chemokines, Limited Pulmonary Vascular Bed Permeability, and... Wang, Erjian; Simard, Marie; Ouellet, Nathalie; Bergeron, Yves; Beauchamp, Denis; Bergeron, Michel G. // Journal of Infectious Diseases;10/1/2000, Vol. 182 Issue 4, p1255 

    Explores the effects of ceftriaxone and interleukin-10 on host response in a mouse model of lethal pneumococcal pneumonia. Protective effect of IL-10 on lethal or sublethal endotoxin challenge in mice; Value of IL-10 in attenuating inflammatory damage to human lungs; Beneficial role of IL-10 as...

  • Immune Subversion by Mycobacterium tuberculosis through CCR5 Mediated Signaling: Involvement of IL-10. Das, Shibali; Banerjee, Sayantan; Majumder, Saikat; Paul Chowdhury, Bidisha; Goswami, Avranil; Halder, Kuntal; Chakraborty, Urmita; Pal, Nishith K.; Majumdar, Subrata // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Tuberculosis is characterized by severe immunosuppression of the host macrophages, resulting in the loss of the host protective immune responses. During Mycobacterium tuberculosis infection, the pathogen modulates C-C Chemokine Receptor 5 (CCR5) to enhance IL-10 production, indicating the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics