TITLE

Kinetics of chemokine secretion in human macrophages infected with various strains of Mycobacterium tuberculosis

AUTHOR(S)
Rajavelu, P.; Das, S. D.
PUB. DATE
July 2010
SOURCE
Indian Journal of Medical Microbiology;Jul2010, Vol. 28 Issue 3, p201
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and Purpose: It has been shown that chemokine secretion upon infection with Mycobacterium tuberculosis is influenced by the virulence of the strain, and it is suggested that virulence-associated differences in chemokine secretion contribute to the failure in containing the infection due to poor granuloma formation. Materials and Methods: In this study, we used prevalent M tuberculosis clinical strains (S7 and S10) to study the chemokine secretion profile in infected THP-1 cells and monocyte-derived macrophages (MDM) and compared this with the chemokine secretion induced by laboratory strains. Results: This study showed that comparatively lower levels of IP-10 were induced by clinical strains than by laboratory strains in both differentiated THP-1 and MDMs. The secretion of MIP-1a was also depressed but only in the THP-1 cells infected with clinical strains. This depressed chemokine secretion may hinder the movement of Th-1 cells from the periphery into the infection foci to control the infection. Correlation between IP-10 and IL-12p40 showed a negative relationship in control MDMs, while there was a positive correlation in all the infected strains, indicating their cooperative role in attracting and activating Th1 cells for a protective immune response at the site. This relationship was strain dependent, with avirulent H37Ra showing higher correlation, followed by the clinical strains and the virulent H37Rv. A positive correlation of IP-10 with IFN-γ (S7 and H37Ra) and with IL-10 (H37Ra and H37Rv) suggested a definitive interplay of these molecules in infection. Conclusions: The chemokines secretion by infected THP-1 cells and MDMs was strain dependant and the lower induction by the clinical strains may indicate that the clinical strains maintain a quiescent nature to mislead the host immune system for their benefit.
ACCESSION #
52597051

 

Related Articles

  • Interleukin-12 and Interleukin-27 Regulate Macrophage Control of Mycobacterium tuberculosis. Robinson, Cory M.; Nau, Gerard J. // Journal of Infectious Diseases;8/1/2008, Vol. 198 Issue 3, p359 

    Mycobacterium tuberculosis is an intracellular pathogen that persists within macrophages and remains a considerable global threat to human health. The purpose of this study was to investigate how interleukin (IL)-12 and IL-27 regulate human macrophage interactions with M. tuberculosis....

  • IL10 and IL12B polymorphisms each influence IL-12p70 secretion by dendritic cells in response to LPS. Peng, Judy C.; Abu Bakar, Sazaly; Richardson, Michelle M.; Jonsson, Julie J.; Frazer, Ian H.; Nielsen, Lars K; Morahan, Grant; Thomas, Ranjeny // Immunology & Cell Biology;Apr2006, Vol. 84 Issue 2, p227 

    Dendritic cells (DC) are the main producers of the cytokine IL-12p70, through which they play a direct role in the development of IFN-γ-secreting Th1 cells, costimulation of CTL differentiation and NK-cell activation. In contrast, IL-10, which is also produced by DC, negatively regulates...

  • IL-8, IL-12 and IL-10 cytokines generation by neutrophils, fibroblasts and neutrophils- fibroblasts interaction in psoriasis. Glowacka, E.; Lewkowicz, P.; Rotsztejn, H.; Zalewska, A. // Advances in Medical Sciences (De Gruyter Open);2010, Vol. 55 Issue 2, p254 

    Purpose: Psoriasis is a common skin disease affecting about 1-3% of the world population. Many types of cells, including lymphocytes, dendritics APCs (antigen presenting cells), NKT (natural killer T) cells, neutrophils, keratinocytes and fibroblasts are involved in the pathogenesis of...

  • Regulatory Effect of SOCS on NF-κB Activity in Murine Monocytes/Macrophages. Park, Sun-Hee; Kim, Kyoung-Eun; Hwang, Ha-Young; Kim, Tae Yoon // DNA & Cell Biology;Feb2003, Vol. 22 Issue 2, p131 

    The suppressor of cytokine signaling (SOCS) group of proteins has been implicated in regulation of various cytokine signaling and in a negative crosstalk between distinct signaling pathways. Interleukin-10 (IL-10) and LPS were known to induce expression of SOCS-3 in neutrophils and...

  • Human Macrophage Responses to Clinical Isolates from the Mycobacterium tuberculosis Complex Discriminate between Ancient and Modern Lineages. Portevin, Damien; Gagneux, Sébastien; Comas, Iñaki; Young, Douglas // PLoS Pathogens;Mar2011, Vol. 7 Issue 3, preceding p1 

    No abstract available.

  • Cellular and Humoral Mechanisms Involved in the Control of Tuberculosis. Zuñiga, Joaquin; Torres-García, Diana; Santos-Mendoza, Teresa; Rodriguez-Reyna, Tatiana S.; Granados, Julio; Yunis, Edmond J. // Clinical & Developmental Immunology;2012, p1 

    Mycobacterium tuberculosis (Mtb) infection is a major international public health problem. One-third of the world's population is thought to have latent tuberculosis, a condition where individuals are infected by the intracellular bacteria without active disease but are at risk for reactivation,...

  • Protective Immunity to Mycobacterium tuberculosis Infection by Chemokine and Cytokine Conditioned CFP- 10 Differentiated Dendritic Cells. Salam, Nasir; Gupta, Shashank; Sharma, Sachin; Pahujani, Shweta; Sinha, Aprajita; Saxena, Rajiv K.; Natarajan, Krishnamurthy // PLoS ONE;2008, Vol. 3 Issue 8, p1 

    Background: Dendritic cells (DCs) play major roles in mediating immune responses to mycobacteria. A crucial aspect of this is the priming of T cells via chemokines and cytokines. In this study we investigated the roles of chemokines RANTES and IP- 10 in regulating protective responses from...

  • Immune Subversion by Mycobacterium tuberculosis through CCR5 Mediated Signaling: Involvement of IL-10. Das, Shibali; Banerjee, Sayantan; Majumder, Saikat; Paul Chowdhury, Bidisha; Goswami, Avranil; Halder, Kuntal; Chakraborty, Urmita; Pal, Nishith K.; Majumdar, Subrata // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Tuberculosis is characterized by severe immunosuppression of the host macrophages, resulting in the loss of the host protective immune responses. During Mycobacterium tuberculosis infection, the pathogen modulates C-C Chemokine Receptor 5 (CCR5) to enhance IL-10 production, indicating the...

  • Modulation of Cytokines and Chemokines, Limited Pulmonary Vascular Bed Permeability, and... Wang, Erjian; Simard, Marie; Ouellet, Nathalie; Bergeron, Yves; Beauchamp, Denis; Bergeron, Michel G. // Journal of Infectious Diseases;10/1/2000, Vol. 182 Issue 4, p1255 

    Explores the effects of ceftriaxone and interleukin-10 on host response in a mouse model of lethal pneumococcal pneumonia. Protective effect of IL-10 on lethal or sublethal endotoxin challenge in mice; Value of IL-10 in attenuating inflammatory damage to human lungs; Beneficial role of IL-10 as...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics