Analysis of intact ladderane phospholipids, originating from viable anammox bacteria, using RP-LC-ESI-MS

Lanekoff, Ingela; Karlsson, Roger
August 2010
Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 8, p3543
Academic Journal
Since the discovery of the anaerobic ammonium oxidizing (anammox) bacteria, many attempts have been made in order to identify these environmentally important bacteria in natural environments. Anammox bacteria contain a unique class of lipids, called ladderane lipids and here we present a novel method to detect viable anammox bacteria in sediments and waste water treatment plants based on the use of a ladderane lipid biomarker. Intact ladderane phosphatidylcholine (PC) lipids are analyzed using reversed-phase liquid chromatography–electrospray ionization–mass spectrometry. Following extraction from the complex sediment matrix, reversed-phase LC is used to separate ladderane PC lipids based on their tail group hydrophobicity as well as their ether or ester link to the glycerol backbone in the sn-2 position. We investigate the presence of intact ladderane lipids in natural sediments displaying anammox activity and illustrate the use of a specific intact membrane forming PC lipid as a biomarker for viable anammox bacterial cells. The presented method can be used to elucidate the whereabouts of viable anammox bacteria, subsequently enabling an estimation of anammox activity. This will greatly increase the knowledge of anammox bacteria and their importance in the global nitrogen cycle.


Related Articles

  • The influence of citrate, EDTA, and heparin anticoagulants to human plasma LC-MS lipidomic profiling. Gonzalez-Covarrubias, Vanessa; Dane, Adrie; Hankemeier, Thomas; Vreeken, Rob // Metabolomics;Apr2013, Vol. 9 Issue 2, p337 

    Lipid profiling of human plasma by liquid chromatography-electrospray ionization coupled to mass spectrometry (LC-ESI-MS) is being used to identify biomarkers of health, disease, and treatment efficacy. However, there is no consensus on the choice of anticoagulant to perform and compare...

  • Instability of the cellular lipidome with age. Hughes, Jessica; Deeley, Jane; Blanksby, Stephen; Leisch, Friedrich; Ellis, Shane; Truscott, Roger; Mitchell, Todd // Age;Aug2012, Vol. 34 Issue 4, p935 

    The human lens nucleus is formed in utero, and from birth onwards, there appears to be no significant turnover of intracellular proteins or membrane components. Since, in adults, this region also lacks active enzymes, it offers the opportunity to examine the intrinsic stability of macromolecules...

  • Surfactant Lipidomics in Healthy Children and Childhood Interstitial Lung Disease. Griese, Matthias; Kirmeier, Hannah G.; Liebisch, Gerhard; Rauch, Daniela; Stückler, Ferdinand; Schmitz, Gerd; Zarbock, Ralf; null, null // PLoS ONE;Feb2015, Vol. 10 Issue 2, p1 

    Background: Lipids account for the majority of pulmonary surfactant, which is essential for normal breathing. We asked if interstitial lung diseases (ILD) in children may disrupt alveolar surfactant and give clues for disease categorization. Methods: Comprehensive lipidomics profiles of...

  • Using high-performance liquid chromatography/mass spectrometry for the quantification of plasma phospholipids in children with type 1 diabetes. Akmurzina, V.; Petryaikina, E.; Saveliev, S.; Selishcheva, A. // Journal of Analytical Chemistry;Dec2013, Vol. 68 Issue 14, p1170 

    Quantification of the plasma phospholipids composition in children with different stages of type 1 diabetes was performed using HPLC/MS with electrospray ionization. Reliable ( p < 0.05) changes in the concentrations of both the general phospholipid classes and individual molecular species were...

  • Determination of azide in gastric fluid and urine by flow-injection electrospray ionization tandem mass spectrometry. Minakata, Kayoko; Nozawa, Hideki; Yamagishi, Itaru; Gonmori, Kunio; Hasegawa, Koutaro; Suzuki, Masako; Watanabe, Kanako; Suzuki, Osamu // Analytical & Bioanalytical Chemistry;Oct2012, Vol. 403 Issue 7, p1793 

    A rapid method was developed to identify and quantify the azide ion (N) in gastric fluid and urine. N in diluted biological fluids was reacted with NaAuCl to produce Au(N), which was extracted with octanol. Five microliters of the extract were flow-injected into an electrospray ionization tandem...

  • Deuterium-exchange metabolomics identifies N-methyl lyso phosphatidylethanolamines as abundant lipids in acidophilic mixed microbial communities. Fischer, Curt; Wilmes, Paul; Bowen, Benjamin; Northen, Trent; Banfield, Jillian // Metabolomics;Aug2012, Vol. 8 Issue 4, p566 

    Natural microbial communities are extremely diverse and contain uncharacterized but functionally important small molecules. By coupling a deuterium (D) labeling technique to high mass accuracy untargeted liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) metabolomic...

  • The Effect of Exercise on the Skeletal Muscle Phospholipidome of Rats Fed a High-Fat Diet. Mitchell, Todd W.; Turner, Nigel; Else, Paul L.; Hulbert, Anthony J.; Hawley, John A.; Jong Sam Lee; Bruce, Clinton R.; Blanksby, Stephen J. // International Journal of Molecular Sciences;Oct2010, Vol. 11 Issue 10, p3954 

    The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague- Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group...

  • Sophorolipids: improvement of the selective production by Starmerella bombicola through the design of nutritional requirements. Ribeiro, Isabel; Bronze, M.; Castro, Matilde; Ribeiro, Maria // Applied Microbiology & Biotechnology;Mar2013, Vol. 97 Issue 5, p1875 

    Microtiter plates were used as minireactors to study Starmerella bombicola growth and sophorolipid (SL) production. Compositional analysis of SL mixtures by liquid chromatography with electrospray ionization tandem mass spectrometry showed similar results on SLs produced using the laboratory...

  • Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry. Alberici, Luciane; Oliveira, Helena; Catharino, Rodrigo; Vercesi, Anibal; Eberlin, Marcos; Alberici, Rosana // Analytical & Bioanalytical Chemistry;Dec2011, Vol. 401 Issue 5, p1655 

    Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics