Fasting Upregulates PPARa Target Genes in Brain and Influences Pituitary Hormone Expression in a PPARa Dependent Manner

K�nig, Bettina; Rauer, Christine; Rosenbaum, Susann; Brandsch, Corinna; Eder, Klaus; Stangl, Gabriele I.
January 2009
PPAR Research;2009, Special section p1
Academic Journal
PPARa is a lipid-activable transcription factor that mediates the adaptive response to fasting. Recent data indicate an important role of brain PPARa in physiological functions. However, it has not yet been shown whether PPARa in brain can be activated in the fasting state. Here we demonstrate that fasting of rats increased mRNA concentrations of typical PPARa target genes implicated in �-oxidation of fatty acids (acyl-CoA oxidase, carnitine palmitoyltransferase-1, medium chain acyl-CoA dehydrogenase) and ketogenesis (mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase) in pituitary gland and partially also in frontal cortex and diencephalon compared to nonfasted animals. These data strongly indicate that fasting activates PPARa in brain and pituitary gland. Furthermore, pituitary prolactin and luteinizing hormone-� mRNA concentrations were increased upon fasting in wild-type mice but not in mice lacking PPAR a. For proopiomelanocortin and thyrotropin-�, genotype-specific differences in pituitary mRNA concentrations were observed. Thus, PPARa seems to be involved in transcriptional regulation of pituitary hormones.


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