Biochemical aspects of diapause preparation in ultimate instar of tropical tasar silkworm Antheraea mylitta Drury

Mishra, P. K.; Kumar, D.; Jaiswal, L.; Kumar, Ashutosh; Singh, B. M. K.; Sharan, S. K.; Pandey, J. P.; Prasad, B. C.
December 2009
Journal of Ecophysiology & Occupational Health;Dec2009, Vol. 9 Issue 3/4, p253
The haemolymph biochemical constituents like total protein, free amino acids, trehalose, glycerol and glycogen were estimated in ultimate instar larvae of Daba bivoltine ecorace of tropical tasar silkworm Antheraea mylitta Drury in order to differentiate between non-diapause destined (NDD) and diapause destined (DD) generations and also to relate them with preparation for undergoing diapause. During phagoperiod of ultimate instar, presence of protein (35.119 mg/ml), trehalose (7.530 mg/ml) and glycerol (0.084 mg/ml) was significantly higher in males of DD generation than NDD (protein- 6.384mg/ml; trehalose 4.298 mg/ml; glycerol- 0.029mg/ml). Female larvae of DD generation had significantly higher level of protein (8.739 mg/ml), amino acids (4.976mg/ml), trehalose (7.963mg/ml) and glycerol (0.077 mg/ml) than female larvae of DD generation. A comparison between the sexes indicated that NDD male larvae had higher level of free amino acids than female larvae. Male larvae of DD generation had higher level of protein, free amino acids and trehalose than female larvae, while female larvae had higher level of glycerol than male larvae. It was observed that accumulation of trehalose takes place during mid instar phagoperiod in both NDD and DD generation. The level of protein was higher through out the phagoperiod with fluctuations. Concentration of glycogen was lower at the beginning of phagoperiod which gradually increases till it maturates in both the generations. The concentration of glycerol was higher during the beginning of instar and before gut purging. During the spinning period biochemical constituents, especially, amino acids (1.683 mg/ml), trehalose (34.857 mg/ml) and glycerol (240.833 microgram/ml) and glycogen (34.857mg/ml) were significantly higher in the females of DD generation than NDD (amino acids 0.856mg/ml; trehalose 15,858mg/ml; glycogen 3.913mg/ml; glycerol- 0.029mg/ml). DD female larvae had significantly higher level of amino acids (0.1.718mg/ml), glycogen (5.967mg/ ml) and glycerol (246.835 microgram/ml) than male larvae of DD generation. Significantly higher level of amino acid, proteins, trehalose, glycogen and glycerol were present in the haemolymph of males than females during non-diapause destined generation. Higher level of protein, amino acids and glycerol during ultimate instar male and female larvae of DD generation is indicative of their preparation for undergoing diapause in pupal stage as tasar silkworms build up its energy-reserve to skip unfavourable environmental conditions.


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