TITLE

Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli

AUTHOR(S)
Jurkevica, Agnese; Halsall, H. Brian; Seliskar, Carl J.; Heineman, William R.
PUB. DATE
August 2010
SOURCE
Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 7, p3133
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β- d-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.
ACCESSION #
52299517

 

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