TITLE

Reversibly sealed multilayer microfluidic device for integrated cell perfusion and on-line chemical analysis of cultured adipocyte secretions

AUTHOR(S)
Clark, Anna M.; Sousa, Kyle M.; Chisolm, Claire N.; MacDougald, Ormond A.; Kennedy, Robert T.
PUB. DATE
August 2010
SOURCE
Analytical & Bioanalytical Chemistry;Aug2010, Vol. 397 Issue 7, p2939
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A three-layer microfluidic device was developed that combined perfusion of cultured cells with on-line chemical analysis for near real-time monitoring of cellular secretions. Two layers were reversibly sealed to form a cell chamber that allowed cells grown on coverslips to be loaded directly into the chip. The outlet of the chamber was in fluidic contact with a third layer that was permanently bonded. Perfusate from the cell chamber flowed into this third layer where a fluorescence enzyme assay for non-esterified fatty acid (NEFA) was performed on-line. The device was used to monitor efflux of NEFAs from ∼6,200 cultured adipocytes with 83 s temporal resolution. Perfusion of murine 3T3-L1 cultured adipocytes resulted in an average basal concentration of 24.2 ± 2.4 μM NEFA (SEM, n = 6) detected in the effluent corresponding to 3.31 × 10−5 nmol cell−1 min−1. Upon pharmacological treatment with a β-adrenergic agonist to stimulate lipolysis, a 6.9 ± 0.7-fold (SEM, n = 6) sustained increase in NEFA secretion was observed. This multilayer device provides a versatile platform that could be adapted for use with other cell types to study corresponding cellular secretions in near real-time.
ACCESSION #
52299506

 

Related Articles

  • Culturing and investigation of stress-induced lipid accumulation in microalgae using a microfluidic device. Holcomb, Ryan E.; Mason, Lucas J.; Reardon, Kenneth F.; Cropek, Donald M.; Henry, Charles S. // Analytical & Bioanalytical Chemistry;Jun2011, Vol. 400 Issue 1, p245 

    There is increasing interest in using microalgae as a lipid feedstock for the production of biofuels. Lipids used for these purposes are triacylglycerols that can be converted to fatty acid methyl esters (biodiesel) or decarboxylated to 'green diesel.' Lipid accumulation in most microalgal...

  • Beyond Counting: New Way To Use Circulating Tumor Cells. Azvolinsky, Anna // JNCI: Journal of the National Cancer Institute;Oct2014, Vol. 106 Issue 10, p1 

    The article discusses a research study published in the 2013 issue of the journal "Science Translational Medicine," in which researchers demonstrated the use of the CTC-iChip microfluidic device to separate and count and profile individual circulating tumor cells (CTC) at the molecular level....

  • Precision trapping on a microfluidic chip. R. M. W. // Physics Today;Jun2014, Vol. 67 Issue 6, p21 

    The article discusses research on precision trapping on a microfluid chip and references a study by Cornell University researchers led by Michelle Wang who have developed an optoelectrical fluidic platform that requires less power and can capture and manipulate hundreds of molecules simultaneously.

  • Fat: the origins of dividing cells? Marshall, Michael // New Scientist;1/12/2012, Vol. 217 Issue 2899, p6 

    The article discusses the role of fat in the rise of the ability of living things to make copies of itself. Topics include a study by Jack Szostak of the Howard Hughes Medical Institute in Boston, Massachusetts, demonstrating simplified cell division that does not require gene activation, the...

  • Rheonix closes $12.6M financing round. Tampone, Kevin // Business Journal (Central New York);5/28/2010, Vol. 24 Issue 22, p5B 

    The article reports that Rheonix Inc. will use 12.6 million dollars of venture financing to fund the development of applications for testing technology in the health-care through its chip-sized microfluidic device.

  • Microfluidic Device with Artificial Arteries Measures Drugs' Influence on Blood Clotting.  // Bioscience Technology;2014, p1 

    The article evaluates a microfluidic device with artificial arteries, developed by researchers to measure the impact of drugs on blood clotting.

  • Guest Editor Introduction: Special Issue on Nano/Bio-Inspired Applications and Architectures. Mishra, Prabhat // International Journal of Parallel Programming;Aug2009, Vol. 37 Issue 4, p343 

    The article discusses various articles published within the issue, including one by Nageswaran and colleagues on the brain derived vision algorithm and the physiological operating principles of thalamo-cortical brain circuits, one by Zhao and Chakrabarty on the online testing of microfluidic...

  • Inside Lab Invest.  // Laboratory Investigation (00236837);Aug2013, Vol. 93 Issue 8, p856 

    An introduction is presented in which the editor discusses various reports within the issue on topics including evaluation of antifibrotic compounds in scarring, and microfluidic device for gene expression studies.

  • Cancer detection: Tracking roving cancer cells.  // Nature;4/8/2010, Vol. 464 Issue 7290, p817 

    The article reports that Daniel Haber and his co-workers at the Massachusetts General Hospital have developed a microfluidic system which can diagnose tumour cells circulating in the bloodstream of prostate cancer patients.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics