TITLE

Increase in Resistance Rates of H.pylori Isolates to Metronidazole and Tetracycline-Comparison of Three 3-Year Studies

AUTHOR(S)
Siavoshi, Farideh; Saniee, Parastoo; Lati-Navid, Saeid; Massarrat, Sadegh; Sheykholeslami, Arghavan
PUB. DATE
May 2010
SOURCE
Archives of Iranian Medicine (AIM);May2010, Vol. 13 Issue 3, p177
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Antimicrobials have been useful in the treatment of H.pylori-related dyspeptic diseases. However, emergence of resistant strains often decreases the eradication rates of H.pylori infections. Large-scale use of antimicrobials will lead to the diminishment of susceptible strains while allowing resistant survivors to outgrow and spread resistance genes. The aim of this study was to assess the change in antimicrobial resistance rate of H.pylori isolates from 2005 to 2008 and indicate the consequences of indiscriminate and widespread use of antimicrobials against H. pylori- and non-H.pylori-related infections. Methods: A total of 110 H. pylori strains were isolated from dyspeptic patients during 2005 to 2008 and tested for their susceptibility to antimicrobials using the disk diffusion method. MICs were determined for metronidazole (8 μg/mL), tetracycline (0.5 μg/mL), clarithromycin (2 μg/mL), amoxicillin (1 μg/mL) and furazolidone (0.5 μg/mL). Since the rates of resistance to metronidazole and tetracycline were remarkably high, another 50 isolates were tested for their susceptibility to metronidazole at the same MIC (8 μg/mL) and tetracycline at MICs of 0.5,1 and 2 μg/mL. Resistance rates were compared to those obtained in our two previous studies between 1997 - 2000 and 2001 - 2004. Results: The resistance rates of 110 H.pylori isolates to clarithromycin, amoxicillin and furazolidone were 7.3%, 7.3%, and 4.5%, respectively. Among 160 H.pylori isolates, 55.6% exhibited resistance to metronidazole and 38.1% to tetracycline. Discussion: Compared to our two previous studies, the resistance rates of H.pylori isolates to current antimicrobials has changed over time. The change in resistance rates of clarithromycin, amoxicillin and furazolidone was not statistically significant. However, resistance to metronidazole and tetracycline showed a considerable increase from 33 - 36.3% to 55.6% and 0 - 0.7% to 38.1%, respectively. Emergence of resistance due to the intensive use of antibiotics has become a global public health problem. It appears that plasmid-carried genes are involved in the spread of resistance traits among bacteria. Results obtained in this study indicate that the increase in resistance rates of H.pylori isolates to metronidazole and tetracycline could be the indication of indiscriminate and frequent use of antibiotics in Iran.
ACCESSION #
52046073

 

Related Articles

  • Failure of first-line eradication treatment significantly increases prevalence of antimicrobial-resistant Helicobacter pylori clinical isolates. Romano, M.; Lovene, M. R.; Russo, M. I.; Rocco, A.; Salerno, R.; Cozzolino, D.; Pilloni, A. P.; Tufano, M. A.; Vaira, D.; Nardone, D. // Journal of Clinical Pathology;Oct2008, Vol. 61 Issue 10, p1112 

    Objectives: Helicobacter pylori infection is a major health problem worldwide, and effective eradication of the infection is mandatory. The efficacy of recommended eradication regimens is approximately 70%. To avoid treatment failure and the consequent development of secondary resistance(s), it...

  • Emerging antimicrobial resistance pattern of Helicobacter pylori in central Gujarat. Pandya, H. B.; Agravat, Harihar Har; Patel, J. S.; Sodagar, NRK // Indian Journal of Medical Microbiology;Oct-Dec214, Vol. 32 Issue 4, p408 

    Background: Antimicrobial resistance is a growing problem in H. pylori treatment. The study was intended to evaluate the prevalence of resistance amongst 80 H.pylori isolates cultured from biopsy taken during routine endoscopies in 2008-2011. Materials and Methods: 855 gastro duodenal biopsies...

  • Phenotypic and Genetic Characterization of Antimicrobial Profiles of Helicobacter pylori Strains in Cuba. Llanes, Rafael; Soria, Claudia; Nagashima, Shigeo; Kobayashi, Nobumichi; Gala, Angela; Guzmán, Daymi; Feliciano, Onelkis; Valdés, Lidunka; Gutiérrez, Oderay; Fernández, Heriberto; Llop, Alina; Wada, Akihiro // Journal of Health, Population & Nutrition;Apr2010, Vol. 28 Issue 2, p124 

    The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and...

  • Relevance of antibiotic sensitivities in predicting failure of omeprazole, clarithromycin, and tinidazole to eradicate Helicobacter pylori. Moayyedi, Paul; Ragunathan, P. L.; Mapstone, Nic; Axon, Anthony T. R.; Tompkins, David S.; Moayyedi, P; Mapstone, N; Axon, A T; Tompkins, D S // Journal of Gastroenterology;1998, Vol. 33 Issue 2, p160 

    Omeprazole 20 mg once (o.d.) or twice daily (b.d.), clarithromycin 250 mg b.d., and tinidazole 500 mg b.d. for 7 days (OCT) is an effective regimen against Helicobacter pylori, but the effect of 5-nitroimidazole resistance is unclear. We aimed to evaluate this using the disc diffusion technique...

  • The Relationship among Previous Antimicrobial Use, Antimicrobial Resistance, and Treatment Outcomes for Helicobacter pylori Infections. Mcmahon, Brian J.; Hennessy, Thomas W.; Michael Bensler, J.; Bruden, Dana L.; Parkinson, Alan J.; Morris, Julie M.; Reasonover, Alisa L.; Huriburt, Debby A.; Bruce, Michael G.; Sacco, Frank; Butler, Jay C. // Annals of Internal Medicine;9/16/2003, Vol. 139 Issue 6, p463 

    Background: The relationship between previous antimicrobial treatments and infection with drug-resistant Helicobacter pylori is unknown. Objectives: To determine whether previous use of antimicrobial agents predicts subsequent antibiotic resistance of H. pylori and whether resistance affects...

  • "Rescue" regimens after Helicobacter pylori treatment failure. Gisbert, Javier P.; Yamaoka, Yoshio; Shimoyama, Tadashi // World Journal of Gastroenterology;9/21/2009, Vol. 14 Issue 35, p5385 

    Helicobacter pylori (H pylori) infection is the main cause of gastritis, gastroduodenal ulcer disease, and gastric cancer. After more than 20 years of experience in Hpylori treatment, in my opinion, the ideal regimen to treat this infection is still to be found. Currently, apart from having to...

  • Efficacy of metronidazole for the treatment of clarithromycin-resistantHelicobacter pyloriinfection in a Japanese population. Shimoyama, Tadashi; Fukuda, Shinsaku; Mikami, Tatsuya; Fukushi, Michio; Munakata, Akihiro // Journal of Gastroenterology;2004, Vol. 39 Issue 10, p927 

    Background. Eradication of Helicobacter pylori has become a common treatment for several diseases. There is an increase in antibiotic-resistant strains, which causes the failure of eradication. The aim of this study was to investigate the usefulness of metronidazole for the treatment of H....

  • High primary resistance to metronidazole and clarithromycin Helicobacter pylori-strains in adults as compared to children. Kalach, N.; Bergeret, M.; Parlier, D.; Roche, B.; Dupont, C.; Benhamou, P.; Chaussade, S.; Raymond, J. // Gut;Sep2002 Supplement 2, Vol. 51, pA83 

    The purpose of our study was to evaluate the prevalence of primary amoxicillin, metronidazole and clarithromycin resistant Helicobacter pylori (H. pylori) strains in adults and children and to assess their evolution with time. Patients & methods: a prospective study was carried out between 1996...

  • Helicobacter pylori Treatment: A Practical Approach. Vakil, Nimish // American Journal of Gastroenterology;Mar2006, Vol. 101 Issue 3, p497 

    The treatment of Helicobacter pylori infection has become complicated by the lack of new treatment regimens and the emergence of drug resistant strains of H. pylori. Metronidazole and clarithromycin resistance are common in developed countries. Eradication rates have been falling in trials and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics