TITLE

A “paper bridge” system to improve in-vitro propagation of arbuscular mycorrhizal fungi

AUTHOR(S)
Dalpé, Yolande; Seguin, Sylvie
PUB. DATE
June 2010
SOURCE
Botany;Jun2010, Vol. 88 Issue 6, p617
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The in-vitro culture of arbuscular mycorrhizal fungi on excised roots, especially when performed on bi-compartmented Petri dishes, has proven to be an efficient system for the production of root-free fungal material. However, even after the contact between fungal hyphae and the excised roots in the proximal root compartment has occurred, up to several weeks may be required for the fungal runner hyphae to cross the median Petri dish wall and reach the distal fungal compartment. This delay is particularly long for the cultivation of slow-growing strains that usually colonize the substrate less aggressively. The delay is due to the difficulty the runner hyphae have in crossing the median Petri dish wall that separates compartments. To facilitate the passage of the fungus across the median wall, a “paper bridge” system has been devised and tested with a number of arbuscular mycorrhizal fungal strains. This method substantially accelerated fungal propagation and simplified the manipulations necessary. The proposed paper-bridge system is described and its advantages discussed. La culture in vitro des champignons mycorhiziens arbusculaires sur racines excisées, notamment en boîtes de Petri bi-compartimentées, constitue un système efficace de production axénique de champignon. Bien que le contact hyphe-racines s’effectue rapidement dans le compartiment racine, plusieurs semaines peuvent être requises par les hyphes pour traverser dans la partie distale du système. Ce délai, particulièrement long pour les souches à croissance lente et peu agressives, est dûprincipalement à la difficulté pour les hyphes de traverser la cloison centrale qui sépare les compartiments. Afin de remédier à cette difficulté un « pont de papier » testé sur une diversité de souches mycorhiziennes s’est avéré efficace pour accélérer la propagation fongique tout en simplifiant substantiellement les manipulations. Le système est décrit et ses avantages discutés.
ACCESSION #
51991067

 

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