TITLE

Preliminary Immunomodulatory Activity of Aqueous and Ethanolic Leaves Extracts of Ocimum basilicum Linn in Mice

AUTHOR(S)
Dashputre, Neelam L.; Naikwade, Nilofer S.
PUB. DATE
April 2010
SOURCE
International Journal of PharmTech Research;Apr2010, Vol. 2 Issue 2, p1342
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
OBJECTIVE: To study the immunomodulatory activity of aqueous and ethanolic extracts of leaves of Ocimum basilicum Linn. (Family: Lamiaceae) in mice. METHODS: The aqueous and ethanolic extract of leaves of Ocimum basilicum was administered orally at the dosage levels of 400 mg/kg/day body weight in mice. The assessment of immunomodulatory activity on specific and nonspecific immunity were studied by haemagglutination antibody (HA) titer, delayed type hypersensitivity (DTH), neutrophil adhesion test and carbon clearance test. In order to induced immunosuppresion in mice by using cyclophosphamide (100 mg/kg/day, p.o.) and levamisole (50 mg/kg/day, p.o.) used as immunostimulating agents. RESULTS: Oral administration of Ocimum basilicum (OB) showed a significant increase in the production of circulating antibody titer in response to sheep red blood cells (SRBCs). A significant (p<0.01) increase in both primary and secondary HA titer was observed while compared to control group, whereas in cyclophosphamide treated group OB showed significant (p<0.01) increase in HA titer. OB showed significantly (p<0.01) potentiated the DTH reaction by facilitating the footpad thickness response to SRBCs in sensitized mice. Also OB evoked a significant (p<0.01) increase in percentage neutrophil adhesion to nylon fibres and phagocytic activity. CONCLUSION: The study demonstrates that OB triggers both specific and non-specific responses to a greater extent. The study comprised the acute toxicity and preliminary phytochemical screening of OB. From the results obtained and phytochemical studies the immunostimulant effect of OB could be attributed to the flavonoid content.
ACCESSION #
51973576

 

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