Fiber scaffolds of polysialic acid via electrospinning for peripheral nerve regeneration

Assmann, Ulrike; Szentivanyi, Andreas; Stark, Yvonne; Scheper, Thomas; Berski, Silke; Dräger, Gerald; Schuster, Robert H.
July 2010
Journal of Materials Science: Materials in Medicine;Jul2010, Vol. 21 Issue 7, p2115
Academic Journal
Fiber scaffolds of bioactive polysialic acid have been prepared via electrospinning for peripheral nerve regeneration. The diameter, morphology and alignment of fibers in scaffolds were adjusted by variation of electrospinning parameters, which are decisive for the cell-scaffold interaction. Due to the high water solubility of polysialic acid (poly-α-2,8- N-acetylneuraminic acid) a photoactive derivative (poly-α-2,8- N-pentenoylneuraminic acid) was used to obtain stable fiber scaffolds in water by photochemical crosslinking. At the optimized fiber scaffolds good cell viability and directed cell proliferation along the fibers was achieved by cell tests with immortalized Schwann cells.


Related Articles

  • A mechanism for morphogen-controlled domain growth. P. Maini // Journal of Mathematical Biology;May2007, Vol. 54 Issue 5, p597 

    Abstract  Many developmental systems are organised via the action of graded distributions of morphogens. In theDrosophilawing disc, for example, recent experimental evidence has shown that graded expression of the morphogen Dpp controls cell proliferation and hence disc growth....

  • Ruffling membrane, stress fiber, cell spreading and proliferation abnormalities in human Schwannoma cells. Pelton, Patricia D; Sherman, Larry S; Rizvi, Tilat A; Marchionni, Mark A; Wood, Patrick; Friedman, Rick A; Ratner, Nancy // Oncogene;10/29/98, Vol. 17 Issue 17, p2195 

    Schwannomas are peripheral nerve tumors that typically have mutations in the NF2 tumor suppressor gene. We compared cultured schwannoma cells with Schwann cells from normal human peripheral nerves (NHSC). Both cell types expressed specific antigenic markers, interacted with neurons, and...

  • Adenovirus Carrying Gene Encoding Haliotis discus discus Sialic Acid Binding Lectin Induces Cancer Cell Apoptosis. Xinyan Yang; Liqin Wu; Xuemei Duan; Lianzhen Cui; Jingjing Luo; Gongchu Li // Marine Drugs;Jul2014, Vol. 12 Issue 7, p3994 

    Lectins exist widely in marine bioresources such as bacteria, algae, invertebrate animals and fishes. Some purified marine lectins have been found to elicit cytotoxicity to cancer cells. However, there are few reports describing the cytotoxic effect of marine lectins on cancer cells through...

  • cytostatic.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p574 

    A definition of the term "cytostatic" is presented, which refers to a state that prevents cell growth and cell proliferation.

  • Effects of Bone Marrow Stromal Cell-conditioned Medium on Primary Cultures of Peripheral Nerve Tissues and Cells. Jiajiong Yang; Hong Wu; Nan Hu; Xiaosong Gu; Fei Ding // Neurochemical Research;Sep2009, Vol. 34 Issue 9, p1685 

    Implantation of bone marrow stromal cells (MSCs) produces an improved functional outcome of peripheral nerve repair. In this study, rat dorsal root ganglion (DRG) explants, rat DRG neurons, and rat Schwann cells (SCs) were treated with monkey MSC-conditioned medium, respectively, and then...

  • Bone marrow-derived fibroblast growth factor-2 induces glial cell proliferation in the regenerating peripheral nervous system. Ribeiro-Resende, Victor Tulio; Carrier-Ruiz, Alvaro; Lemes, Robertha M R; Reis, Ricardo A M; Mendez-Otero, Rosalia // Molecular Neurodegeneration;2012, Vol. 7 Issue 1, p34 

    Background: Among the essential biological roles of bone marrow-derived cells, secretion of many soluble factors is included and these small molecules can act upon specific receptors present in many tissues including the nervous system. Some of the released molecules can induce proliferation of...

  • Podoplanin and CD34 in peripheral nerve sheath tumours: focus on neurofibromatosis 1-associated atypical neurofibroma. Naber, Urs; Friedrich, Reinhard; Glatzel, Markus; Mautner, Victor; Hagel, Christian // Journal of Neuro-Oncology;Jun2011, Vol. 103 Issue 2, p239 

    Patients suffering from neurofibromatosis type 1 and 2 (NF1 and NF2) are the main risk groups to develop peripheral nerve sheath tumours (PNSTs). In the present study, adhesion molecules CD34 and podoplanin were assessed in regard to their value for tumour classification and as indicators for...

  • Loss of SOX10 function contributes to the phenotype of human Merlin-null schwannoma cells. Doddrell, Robin D. S.; Dun, Xin-Peng; Shivane, Aditya; Feltri, M. Laura; Wrabetz, Lawrence; Wegner, Michael; Sock, Elisabeth; Hanemann, C. Oliver; Parkinson, David B. // Brain: A Journal of Neurology;Feb2013, Vol. 136 Issue 2, p549 

    Loss of the Merlin tumour suppressor causes abnormal de-differentiation and proliferation of Schwann cells and formation of schwannoma tumours in patients with neurofibromatosis type 2. Within the mature peripheral nerve the normal development, differentiation and maintenance of myelinating and...

  • Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems. Hua, Zhong L.; Emiliani, Francesco E.; Nathans, Jeremy // Neural Development;10/23/2015, Vol. 10, p1 

    Background: Rac1 is a critical regulator of cytoskeletal dynamics in multiple cell types. In the nervous system, it has been implicated in the control of cell proliferation, neuronal migration, and axon development. Results: To systematically investigate the role of Rac1 in axon growth and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics