TITLE

An UV-Visible Spectrophotometric Method for the Estimation of Plumbagin

AUTHOR(S)
Israni, Shalini A.; Kapadia, Nayana S.; Lahiri, Suman. K.; Yadav, Gunvat K.; Shah, Mamta B.
PUB. DATE
April 2010
SOURCE
International Journal of ChemTech Research;Apr2010, Vol. 2 Issue 2, p856
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A simple, rapid and sensitive spectrophotometric method for estimation of plumbagin in the roots of P. rosea is developed. The present investigation is further aimed at determination of the plumbagin content in the roots of different Plumbaginales namely, Plumbago zeylanica, P. capensis, P. capensis var. alba and Vogelia indica employing the same method. V. indica is widely used as an alternative drug to replace P. rosea. Studies in the past have reported the presence of plumbagin in P. rosea, P. zeylanica and P. capensis. Determination of plumbagin content in P. capensis var. alba and V. indica is reported in this work for the first time. Although, literature review details the application of HPTLC for estimation of plumbagin, the current work is an effort to develop an alternative method to determine the plumbagin content. The proposed spectrophotometric method is based on formation of magenta pink colored phenolate ion by reaction of naphthoquinones with alkalies. A graph of absorbance versus concentration shows that Beer's law is obeyed in the concentration range of 40μg/ml-240μg/ml. The pink colored chromogen is measured at maximum wavelength of 520nm. The results of analysis have been validated statistically and by recovery studies. The study revealed that the bioactive marker was found to be maximum in P. rosea (1.68%) followed by P. zeylanica (0.413%), P. capensis var. alba (0.33%), P. capensis (0.297%), and lastly V. indica (0.13%).
ACCESSION #
51924372

 

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