TITLE

Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra L. Hypocotyl Explants

AUTHOR(S)
Hussein, E. A.; Tai-Eldeen, A. M.; Al-Zubairi, A. S.; Elhakimi, A. S.; Al-Dubaie, A. R.
PUB. DATE
July 2010
SOURCE
International Journal of Pharmacology;2010, Vol. 6 Issue 4, p446
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In the present study, callus was successfully obtained from hypocotyl explants of Brassica nigra (L.) in vitro under both dark and light incubation conditions separately using Murashige and Skoog's basal salts enriched with sucrose, benzyladenine (BA), Naphthalene Acetic Acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4 D). Callus growth curve was plotted on the basis of single callus dry weight in grams versus time in weeks. The obtained calli were subjected to phytochemical screening for some secondary plant metabolites using thin layer chromatography, determination of total phenohcs using Folm Ciocalteu reagent, estimation of the antioxidant activity by the scavenging activity of DPPH free radicals and screening calli extracts for antibacterial activity against 4 pathogenic bacterial strains. Except for phytochemical screening which was carried out at the end of the growth period only, other analyses were carried out when calli were 2, 4 and 6 weeks old. Results of the present study have revealed that calli were rich in secondary metabolites as they gave positive results for testes of volatile oils, anthraquinones, flavonoids and tannins. Although there were some exceptions, it was generally observed that total phenolic, antioxidant activity and antibacterial activity were higher in calli obtained under light incubation conditions than calli obtained under dark incubation conditions or the mother plant parts from which calli were induced. It is also observed that older calli accumulated more amounts of total phenohcs, exhibited higher antioxidant activity and stronger antibacterial activity.
ACCESSION #
51910980

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics