Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?

Das, Sujata; Sharma, Savitri; Kar, Sarita; Sahu, Srikant K.; Samal, Bikash; Mallick, Aparajita
June 2010
Indian Journal of Ophthalmology;Jun2010, Vol. 58 Issue 4, p281
Academic Journal
Purpose: To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. Materials and Methods: Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with Escherichia coli overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. Results: Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culturepositive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, P = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in in vitro experiment although the characteristic spores and color were seen earlier on SDA. Conclusion: Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.


Related Articles

  • Chronic hepatitis C virus infection is not associated with Mooren's ulcer. Wang, Q.-S.; Yuan, J.; Zhou, S.-Y.; Chen, J.-Q. // Eye;May2008, Vol. 22 Issue 5, p697 

    AimTo investigate the association between chronic hepatitis C virus (HCV) infection and Mooren's ulcer.MethodsEight patients from different parts of China who were diagnosed with Mooren's ulcer at the Zhongshan Ophthalmic Center, Guangzhou (China) were screened for chronic HCV infection....

  • Haemolytic Fungi Isolated from Sago Starch in Papua New Guinea. Barry Blaney; Mary Fletcher; Jeffrey Warner // Mycopathologia;Feb2010, Vol. 169 Issue 2, p107 

    Abstract  Sago haemolytic disease (SHD) is a rare but often fatal illness linked to consumption of stale sago starch in Papua New Guinea. Although the aetiology of SHD remains unknown, mycotoxins are suspected. This study investigated whether fungi isolated from Papua New Guinean sago...

  • Dendrites threaten vision. Chynn, Emil W. // Ophthalmology Times;3/20/95, Vol. 20 Issue 12, p33 

    Presents a diagnosis for bilateral acanthamoeba keratitis. Foreign body sensation in both eyes; Rinsing with tap water without removing contact lenses; Previous treatment.

  • Rare case of fungal keratitis caused by Plectosporium tabacinum. Kamada, Rika; Monden, Yu; Uehara, Koji; Yamakawa, Ryoji; Nishimura, Kazuko // Clinical Ophthalmology;2012 Part 2, Vol. 6, p1623 

    A rare case of fungal keratitis caused by Plectosporium tabacinum is reported. A 78-year-old female gardener presented with conjunctivitis and an oval infiltrate with irregular margins in the nasal half of the cornea in the right eye. Light microscopy of corneal scrapings revealed a filamentous...

  • IDENTIFICATION OF FUNGAL KERATITIS AGENTS IN THE EAST OF MAZANDARAN AREA BY PCR AND DETERMINATION OF THEIR DRUG SENSITIVITY. Nikpour, Shaghayegh; Omran, Ayatollah Nasrollahi; Omran, Saeid Mahdavi; Karmi, Azar // Jundishapur Journal of Microbiology;2013 Special Edition, p77 

    Introduction and Objectives: Fungal infection causes extensive infective ocular ulcer worldwide and can due to morbidity and blindness. Keratitis is prevalent in tropical and developing countries. For diagnosis and treatment fungal infection is one of the most challenging causes of corneal...

  • MORPHOLOGICAL ASPECTS OF FRUITING BODIES IN MICROSPORUM GYPSEUM ON SABOUREAUD`S DEXTROSE AGAR MEDIUM. Mihali, C. V.; Buruiana, A.; Turcus, Violeta; Covaci, Aurelia; Ardelean, A. // Annals of the Romanian Society for Cell Biology;2011, Vol. 16 Issue 2, p85 

    Microsporum gypseum belongs to Arthrodermataceae family, order Onygenales of Ascomycota division from Fungi. The species teleomorphic phase is represented by Arthroderma gypseum and Arthroderma incurvatum. Soil is the natural environment of the species being which behaves as a dermatophyte, but...

  • Efecto de Fungicidas Sistémicos en el Control de la Micobiota Parasítica del Grano de Sorgo [Sorghum bicolor (L.) Moench]. Montes-García, Noé; Prom, Louis K.; Montes-Rodríguez, Noé; García-Gracia, Miguel Ángel; Pecina-Quintero, Victor; Díaz-Franco, Arturo // Revista Mexicana de Fitopatología;2010, Vol. 28 Issue 2, p156 

    Triazolic fungicides were applied to sorghum genotypes at several rates during the grain filling period to determine the control of grain mold. After harvest, grain samples were taken and put in potato dextrose agar. Fungicide application in the anthesis stage promoted more healthy seeds...

  • Effect of Alcohol on Bacterial Hemolysis. Shirron, Natali; Korem, Moshe; Shuster, Amir; Leikin-Frenkel, Alicia; Rosenberg, Mel // Current Microbiology;Oct2008, Vol. 57 Issue 4, p318 

    Hemolysis of blood agar is broadly used as a diagnostic tool for identifying and studying pathogenic microorganisms. We have recently shown that alcohol vapors can confer hemolytic properties on otherwise nonhemolytic fungi (microbial alcohol-conferred hemolysis; MACH). Until now, this...

  • Antibiotic Resistance in Enterococcus faecalis Isolated from Hospitalized Patients. Gajan, Esrafil Balaei; Shirmohammadi, Adileh; Aghazadeh, Mohammad; Alizadeh, Mohammad; Deljavan, Alireza Sighari; Ahmadpour, Farzin // Journal of Dental Research, Dental Clinics, Dental Prospects;Spring2013, Vol. 7 Issue 2, p102 

    Background and aims. Enterococci are Gram-positive cocci that often occur in pairs (diplococci) or short chains. Beside developing high level of antibiotic resistance, these bacteria can cause wide range of disease in human, thus to help provide an effective treatment for infections caused by...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics