Near-field recording technique for high-resolution fluorescent imaging

Moriguchi, C.; Inami, W.; Egami, C.; Kawata, Y.; Terakawa, S.; Tsuchimori, M.; Watanabe, O.
June 2010
Applied Physics Letters;6/14/2010, Vol. 96 Issue 24, p243103
Academic Journal
We present near-field fluorescent imaging with high resolution using a near-field recording technique. In this technique, optical intensity distribution localized near specimens is recorded as the surface topographic distribution of a photosensitive film. Because the technique does not require the scanning probes for detecting light, it is possible to observe living biological specimens. Fluorescence imaging is one of important tools in investigations of cell structures and functions in biology. We combined near-field recording technique with fluorescence imaging and demonstrated the observations of fluorescent particles beyond the diffraction limit.


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