Multiplexed identification of different fish species by detection of parvalbumin, a common fish allergen gene: a DNA application of multi-analyte profiling (xMAPâ„¢) technology

Hildebrandt, Sabine
July 2010
Analytical & Bioanalytical Chemistry;Jul2010, Vol. 397 Issue 5, p1787
Academic Journal
Fish are a common cause of allergic reactions associated with food consumption, with parvalbumin being the major allergenic protein. Some fish-hypersensitive patients tolerate some fish species while being allergic to others. Reliable detection methods for allergenic fish species in foods are necessary to ensure compliance with food allergen labeling guidelines to protect fish-allergic consumers. The objective of this project was to develop a multi-analyte detection method for the presence of fish in food. Therefore, conserved parvalbumin exon sequences were utilized for the design of universal PCR primers amplifying intron DNA and small regions of exons flanking the enclosed intron from even very distantly related fish species. An assay for the identification of eight fish species was developed using xMAPâ„¢ technology with probes targeting species-specific parvalbumin intron regions. Additionally, a universal fish probe was designed targeting a highly conserved exon region located between the intron and the reverse primer region. The universal fish assay showed no cross-reactivity with other species, such as beef, pork, lamb, chicken, turkey, and shrimp. Importantly, with the exception of one notable case with fish in the same subfamily, species-specific detection showed no cross-reactivity with other fish species. Limits of detection for these eight species were experimentally estimated to range from 0.01% to 0.04%, with potential to increase the detection sensitivity. This report introduces a newly developed method for the multiplex identification of at least eight allergenic fish species in food, which could conceivably be extended to detect up to 100 species simultaneously in one sample. [Figure not available: see fulltext.]


Related Articles

  • (1 3)- beta -D-Glucan and endotoxin modulate immune response to inhaled allergen. Rylander, R.; Holt, P.G. // Mediators of Inflammation;Apr98, Vol. 7 Issue 2, p105 

    EXPOSURE to dust may involve co-exposure to agents which are allergens, together with those which are pro-inflamm atory. To study the effects of such a coexposure, the humoral and inflammatory responses were studied in guinea pigs inhaling the T-celldependent antigen ovalbumin (OVA) and the...

  • In Vitro Determination of the Allergenic Potential of Egg White in Processed Meat. Hildebrandt, Sabine; Schütte, Larsen; Stoyanov, Stefan; Hammer, Günther; Steinhart, Hans; Paschke, Angelika // Journal of Allergy;2010, p1 

    Hen’s egg white has been reported as a causative agent of allergic reactions, with ovalbumin, conalbumin, ovomucoid, and lysozyme being themajor allergens.However, little is known about the effects of processing with heat and high pressure on the allergenicity of egg white proteins as...

  • Allergy to uncommon pets: new allergies but the same allergens. Díaz-Perales, Araceli; González-de-Olano, David; Pérez-Gordo, Marina; Pastor-Vargas, Carlos // Frontiers in Immunology;Dec2013, Vol. 4, p1 

    The prevalence of exotic pet allergies has been increasing over the last decade. Years ago, the main allergy-causing domestic animals were dogs and cats, although nowadays there is an increasing number of allergic diseases related to insects, rodents, amphibians, fish, and birds, among others....

  • Atheroprotective vaccination with MHC-II restricted peptides from ApoB-100. Tse, Kevin; Gonen, Ayelet; Sidney, John; Hui Ouyang; Witztum, Joseph L.; Sette, Alessandro; Tse, Harley; Ley, Klaus // Frontiers in Immunology;Dec2013, Vol. 4, p1 

    Background: Subsets of CD4+ T-cells have been proposed to serve differential roles in the development of atherosclerosis. Some T-cell types are atherogenic (T-helper type 1), while others are thought to be protective (regulatory T-cells). Lineage commitment toward one type of helper T-cell...

  • Editorial [Hot Topic: New Targets for Prevention and Treatment of Allergies (Guest Editor: Udo Herz)]. Herz, Udo // Current Drug Targets - Inflammation & Allergy;Oct2005, Vol. 4 Issue 5, p521 

    The incidence and prevalence of allergic diseases such as asthma, has increased dramatically over the last decades with major socioeconomic consequences. Although enormous efforts have been undertaken to understand the underlying pathomechanism in more detail, asthma is still one of the diseases...

  • Treatments for Food Allergies Lack Strong Evidence.  // Today's Dietitian;Jul2010, Vol. 12 Issue 7, p55 

    The article presents a study that investigates diagnosis, prevalence and treatments of food allergies in the U.S. Researchers found that food allergies impact more than one to two percent but less than 10% of the population. Moreover, they also discovered that majority of food allergies are...

  • Coping With Seasonal Allergies. Peeke, Pamela // National Women's Health Report;Apr2002, Vol. 24 Issue 2, p8 

    Presents tips in coping with seasonal allergies. Avoidance of pollens and smoking; Reduction of dust particles in home and offices; Importance of cleaning the surroundings of molds, pet hairs and other allergens. INSET: Questions to Ask Your Health Care Professional about Allergies.

  • Could You Be Allergic?  // Current Health 2;Oct2002, Vol. 29 Issue 2, SKIN SMARTS: A TEEN'S... p3 

    Focuses on the factors causing an allergic reaction. Allergens in metal jewelry; Substance found in bath and hygiene products; Causes of natural rubber latex allergies.

  • Patch-test Reactions to Plastic and Glue Allergens. Kanerva, Lasse; Jolanki, Riitta; Alanko, Kristiina; Estlander, Tuula // Acta Dermato-Venereologica;6/23/99, Vol. 79 Issue 4, p296 

    Plastics and glues are common causes of occupational dermatoses, but only few reports have dealt with patch-test reactions caused by plastic and glue allergens. Patients exposed to plastics and remitted to an occupational dermatology clinic, were patch-tested with 50-53 plastic and glue...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics