TITLE

Genetic Variability among Four Egyptian Sheep Breeds Using Random Amplified Polymorphic Dna (RAPD) and PCR-RFLP Techniques

AUTHOR(S)
Abdel-rahman, Salah M.; El-nahas, Abeer F.; Hemeda, Shaaban A.; El-fiky, Said A.; Nasr, Sherif M.
PUB. DATE
January 2010
SOURCE
Journal of Applied Sciences Research;Jan2010, Vol. 6 Issue 1, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Genetic variability among four Egyptian sheep breeds (Barki, Rahmani, Ossimi and Romanov) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Sixteen random primers were used to amplify DNA fragments in these four sheep breeds. RAPD patterns with a level of polymorphism were detected among breeds. The results showed that the genetic similarity among these four breeds was as follows: 90% (Barki x Ossimi), 87% (Barki x Rahmani), 87% (Ossimi x Rahmani), 80% (Ossimi x Romanov), 80% (Rahmani x Romanov) and 74% (Barki x Romanov). However, the closer proximity or the highest genetic similarity was observed between Barki and Ossimi (90%), while the lowest was observed between Barki and Romanov (74%). On the other hand, the results of the RFLP for 18S rRNA gene technique showed that, no genetic variation were found among the four sheep breeds under study. Finding in this study confirms the phenotypic classification of Egyptian sheep to three related breeds (Barki, Ossimi and Rahmani) and the other breed (Romanov) is relatively remote.
ACCESSION #
51408817

 

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