Effects of Pre-soaking Cucurbita Pepo L. (C. Pepo) Seeds in Two Different Concentrations of Epibrassinolide (Eb) on Seed Germination and Seedling Growth

Sayed, Hala F.; Ibrahim, Hemmat K.; El-Shafey, Azza S.; Hassan, Heba M.
October 2009
Australian Journal of Basic & Applied Sciences;2009, Vol. 3 Issue 4, p4465
Academic Journal
Enhanced germination and growth of C.pepo seedling in response to a relatively low concentration of epibrassinolide (EB) was envisaged in terms of increased metabolic activities which were recorded in a decreased content of soluble sugars and polysaccharides of both the cotyledons and the seedling. Moreover, there was a noticeable migration of the soluble nitrogenous fractions, the amino acids and DNA from the cotyledons towards the sinks of the actively growing seedlings in response to the same treatment, in addition to a significant increase in RNA content of both the cotyledons and the seedlings. Accumulation of high level of P,Ca and Mg in cotyledons as a result of seed soaking in relatively low concentration of EB had contributed in enhancing the mobilization of reserves of cotyledons participating in its accessibility to translocation to the developed seedlings. Application of a relatively high concentration of EB retarded seed germination and seedling growth of C.pepo via accumulating carbohydrate fractions in cotyledons and decreasing their availability for growth requirement of seedling. Also this treatment led to amarked decrease in soluble nitrogenous fractions, amino acids,DNA and RNA in both cotyledons and seedling. Moreover, the accumulation of the element Mg,Ca and P in the cotyledons prevented its participation in the metabolic activities dependent on these elements .The change in protein banding pattern of C.pepo cotyledons and seedlings in response to any of the applied concentrations of EB revealed a unique pattern if compared with the control.


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