TITLE

In-Silico Identification of Putative p53 Transcription Factor DNA-Binding Sites and Detection of hnRNP Genes Regulated by p53

AUTHOR(S)
Dutta, Avirup; Chatterjee, Raghunath; Roychoudhury, Paromita; Roy, Patanjal; Chaudhuri, Keya
PUB. DATE
June 2010
SOURCE
IUP Journal of Biotechnology;Jun2010, Vol. 4 Issue 2, p7
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An evolutionary ancient transcription factor p53 coordinates diverse cellular responses to stress and damage and plays an emerging role in various physiological processes by regulating the expression of a number of genes of diverse families and pathways. The p53 DNA-binding motif consists of a consensus region of around 34 bases comprising two inverted palindromic repeats separated by a variable spacer region. An algorithm was developed to identify putative binding elements on 503 selected human genes in the promoter regions and subsequently scored using a reference weight matrix. The genes chosen were loosely classified into seven different biological pathways or families related to various cellular and metabolic pathways. About 124 genes were identified as potential p53 targets which had scores above a theoretical cut-off value. A group of hnRNP genes were tested by semiquantative reverse transcription (RT) PCR analysis for p53 responsiveness. Semiquantitative RT-PCR experiments were performed in p53 null cell line H1299 and in p53 transfected H1299 cells. Four out of six genes responded to the presence of p53.
ACCESSION #
51265557

 

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