TITLE

Preparation and characterization of collagen microspheres for sustained release of VEGF

AUTHOR(S)
Nagai, Nobuhiro; Kumasaka, Norihiro; Kawashima, Takeaki; Kaji, Hirokazu; Nishizawa, Matsuhiko; Abe, Toshiaki
PUB. DATE
June 2010
SOURCE
Journal of Materials Science: Materials in Medicine;Jun2010, Vol. 21 Issue 6, p1891
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this study, we prepared injectable collagen microspheres for the sustained delivery of recombinant human vascular endothelial growth factor (rhVEGF) for tissue engineering. Collagen solution was formed into microspheres under a water-in-oil emulsion condition, followed by crosslinking with water-soluble carbodiimide. Various sizes of collagen microspheres in the range of 1–30 μm diameters could be obtained by controlling the surfactant concentration and rotating speed of the emulsified mixture. Particle size proportionally decreased with increasing the rotating speed (1.8 μm per 100 rpm increase in the range of 300–1,200 rpm) and surfactant concentration (3.1 μm per 0.1% increase in the range of 0.1–0.5%). The collagen microspheres showed a slight positive charge of 8.86 and 3.15 mV in phosphate-buffered saline and culture medium, respectively. Release study showed the sustained release of rhVEGF for 4 weeks. Released rhVEGF was able to induce capillary formation of human umbilical vein endothelial cells, indicating the maintenance of rhVEGF bioactivity after release. In conclusion, the results suggest that the collagen microspheres have potential for sustained release of rhVEGF.
ACCESSION #
51242275

 

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