Impacts of recent cultivation on genetic diversity pattern of a medicinal plant, Scutellaria baicalensis (Lamiaceae)

Qing-Jun Yuan; Zhi-Yong Zhang; Juan Hu; Lan-Ping Guo; Ai-Juan Shao; Lu-Qi Huang
January 2010
BMC Genetics;2010, Vol. 11, p29
Academic Journal
Background: Cultivation of medicinal plants is not only a means for meeting current and future demands for large volume production of plant-based drug and herbal remedies, but also a means of relieving harvest pressure on wild populations. Scutellaria baicalensis Georgi (Huang-qin or Chinese skullcap) is a very important medicinal plant in China. Over the past several decades, wild resource of this species has suffered rapid declines and large-scale cultivation was initiated to meet the increasing demand for its root. However, the genetic impacts of recent cultivation on S. baicalensis have never been evaluated. In this study, the genetic diversity and genetic structure of 28 wild and 22 cultivated populations were estimated using three polymorphic chloroplast fragments. The objectives of this study are to provide baseline data for preserving genetic resource of S. baicalensis and to evaluate the genetic impacts of recent cultivation on medicinal plants, which may be instructive to future cultivation projects of traditional Chinese medicinal plants. Results: Thirty-two haplotypes of S. baicalensis (HapA-Y and Hap1-7) were identified when three chloroplast spacers were combined. These haplotypes constituted a shallow gene tree without obvious clusters for cultivated populations, suggesting multiple origins of cultivated S. baicalensis. Cultivated populations (hT = 0.832) maintained comparable genetic variation with wild populations (hT = 0.888), indicating a slight genetic bottleneck due to multiple origins of cultivation. However, a substantial amount of rare alleles (10 out of 25 haplotypes within wild populations) lost during the course of S. baicalensis cultivation. The genetic differentiation for cultivated group (GST = 0.220) was significantly lower than that of wild group (GST = 0.701). Isolation by distance analysis showed that the effect of geographical isolation on genetic structure was significant in wild populations (r = 0.4346, P < 0.0010), but not in cultivated populations (r = 0.0599, P = 0.2710). These genetic distribution patterns suggest that a transient cultivation history and the extensive seed change among different geographical areas during the course of S. baicalensis cultivation. Conclusions: Although cultivated S. baicalensis maintains comparable genetic diversity relative to wild populations, recent cultivation has still imposed profound impacts on genetic diversity patterns of the cultivated S. baicalensis populations, i.e., the loss of rare alleles and homogenization of cultivated populations. This study suggests that conservation-by-cultivation is an effective means for protecting genetic resources of S. baicalensis, however, the wild resources still need to be protected in situ and the evolutionary consequences of extensive seed exchange mediated by human being should be monitored carefully.


Related Articles

  • Bailcal Skullcap.  // Art of Healing;Mar-May2009, Vol. 1 Issue 26, p40 

    The article provides information on the healing properties of Baical skullcap. It mentions that this plant has been used in some of the countries in Asia, particularly in China, and the Americas in treating several diseases and it contains flavonoids that can alleviate illnesses. It highlights...

  • Baicalein protects mice against radiation-induced DNA damages and genotoxicity. Gandhi, Nitin // Molecular & Cellular Biochemistry;Jul2013, Vol. 379 Issue 1/2, p277 

    Baicalein is the major flavonoid extracted from the root of Scutellaria baicaleins. This flavonoid is used extensively in Chinese herbal medicine. In the present study baicalein is evaluated for its radioprotective properties. Human blood cells when exposed to the γ-radiation ex vivo in...

  • Artificial seed preparation as the efficient method for storage and production of healthy cultured roots of medicinal plants. Vdovitchenko, M.; Kuzovkina, I. // Russian Journal of Plant Physiology;May2011, Vol. 58 Issue 3, p524 

    The technique for the refinement of pRi T-DNA-transformed root cultivation by the root fragment encapsulation in the gel coat, i.e., so-called 'artificial seed' (AS) production, was studied. AS were produced from genetically transformed roots of Baikal skullcap ( Scutellaria baicalensis Georgi)...

  • Advances Research in Callus Inducement and Cell Culture of Scutellaria baicalensis Georgi. FANG Qiang; QIAO Yong-jin // Natural Product Research & Development;Feb2008, Vol. 20 Issue 1, p187 

    Scutellaria baicalensis Georgi is a traditional medicine herb plant. It have many important bioactive effects, such as antibacterial, anti-abnormal reaction, anti-carcinogenic, anti-oxidation, anti-HIV ,eliminating free radical etc. This paper summarized the explant selection, culture condition...

  • Organic acids from medicinal plants. 4. Scutellaria baicalensis. Chirikova, N. K.; Olennikov, D. N.; Rokhin, A. V. // Chemistry of Natural Compounds;Jan2008, Vol. 44 Issue 1, p84 

    The article reports on the study which determines the organic acids (OA) from Scutellaria baicalensis. The study isolated the ditricarboxylic acids, tricarboxylic acids and pure compounds and studied the OA accumulation features during plant development. It is indicated that the group of...

  • MOLECULAR CHARACTERIZATION OF CAROTENOID BIOSYNTHETIC GENES AND CAROTENOID ACCUMULATION IN SCUTELLARIA BAICALENSIS GEORGI. Pham Anh Tuan; Yeon Bok Kim; Jae Kwang Kim; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Sang Un Park // EXCLI Journal;2015, Vol. 14, p146 

    Scutellaria baicalensis has a wide range of biological activities and has been considered as an important traditional drug in Asia and North America for centuries. A partial-length cDNA clone encoding phytoene synthase (SbPSY) and full-length cDNA clones encoding phytoene desaturase (SbPDS),...

  • Water Deficit Affected Flavonoid Accumulation by Regulating Hormone Metabolism in Scutellaria baicalensis Georgi Roots. Yuan Yuan; Yunjun Liu; Chong Wu; Shunqin Chen; Zhouyong Wang; Zhaochun Yang; Shuangshuang Qin; Luqi Huang // PLoS ONE;Oct2012, Vol. 7 Issue 10, Special section p1 

    The content of flavonoids especially baicalin and baicalein determined the medical quality of Scutellaria baicalensis which is a Chinese traditional medicinal plant. Here, we investigated the mechanism responsible for the content and composition of flavonoids in S. baicalensis under water...

  • Genetic diversity in Eucommia ulmoides (Eucommiaceae), an endangered traditional Chinese medicinal plant. Yao, Xiaohong; Deng, Jianyun; Huang, Hongwen // Conservation Genetics;Dec2012, Vol. 13 Issue 6, p1499 

    Eucommia ulmoides, the only species of Eucommiaceae, has been used as Chinese medicinal plant for more than 2,000 years, and is endangered as a consequence of long-term overexploitation. In this study, genetic diversity within and among the semi-wild and cultivated populations of E. ulmoides...

  • Anti-pruritic effect of baicalin and its metabolites, baicalein and oroxylin A, in mice. Hien-trung TRINH; Eun-ha JOH; Ho-young KWAK; Nam-in BAEK; Dong-hyun KIM // Acta Pharmacologica Sinica;Jun2010, Vol. 31 Issue 6, p718 

    AbstractAim:To explore whether intestinal microflora plays a role in anti-pruritic activity of baicalin, a main constituent of the rhizome of Scutellaria baicalensis (SB).Methods:Baicalin was anaerobically incubated with human fecal microflora, and its metabolites, baicalein and oroxylin A, were...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics