TITLE

Utilización de semen bovino sexado en inseminación artificial, transferencia embrionaria y fertilización in vitro

AUTHOR(S)
Oses, M. V.; Teruel, M. T.; Cabodevila, J. A.
PUB. DATE
November 2009
SOURCE
Revista Veterinaria;2009, Vol. 20 Issue 2, p138
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Control of offspring's sex has always been one of the principal objectives in animal production. Since 1992, flow cytometry is used to differentiate between X and Y spermatozoids according to DNA content. Sex determination can be achieved with an accuracy rate higher than 90% and the frequency of calves delivered alive is similar to that observed for conventional semen. The objective of the present work was to analyze the results obtained in artificial insemination (AI), embryo transfer (ET) and in vitro fertilization (IVF) programs when using sexed semen. In AI sexed semen was used mainly with heat detection and the pregnancy rates were between 5-35% lower than those obtained with conventional semen. As to fixed-time artificial insemination (FTAI), use of sexed semen is quite recent and preliminary results are promising; however, comparison to conventional semen results has not been done yet. In ET, sexed semen was used in inseminations with heat observation and FTAI, obtaining results lower than those with conventional semen. Determining of an optimal protocol has not been possible, since reported works used different numbers of inseminations and sexed semen doses. In IVF, sexed semen was used to fertilizing oocytes collected from both slaughter houses and live animals. Percentage of blastocytes was lower than that obtained with unsexed semen, also a higher variability was observed with sexed semen (0-25.8%) compared to unsexed semen (26.3-33.6%). In conclusion, successful utilization of sexed semen in AI, ET and IVF programs is feasible; however, results are generally lower than those achieved when using conventional semen and also influenced by different factors. Further knowledge and control of variables, along with improvement of semen sexing technologies, will determine better and more predictable results.
ACCESSION #
51153051

 

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