Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering

Rao, Satish; Raj, Saurabh; Balint, Stefan; Fons, Carlota Bardina; Campoy, Susana; Llagostera, Montserrat; Petrov, Dmitri
May 2010
Applied Physics Letters;5/24/2010, Vol. 96 Issue 21, p213701
Academic Journal
Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silver colloid particles. The nonspecific binding of the metal to the DNA enhances the Raman scattering that is excited by a near-infrared beam. A Raman spectrum is first recorded followed by a force-extension curve that verifies the presence of a single DNA molecule.


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